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Long-term ammonium nutrition of Arabidopsis increases the extrachloroplastic NAD(P)H/NAD(P)(+) ratio and mitochondrial reactive oxygen species level in leaves but does not impair photosynthetic capacity

Podgorska, Anna; Gieczewska, Katarzyna; Lukawska-Kuzma, Katarzyna; Rasmusson, Allan LU ; Gardestrom, Per and Szal, Bozena (2013) In Plant, Cell and Environment 36(11). p.2034-2045
Abstract
Ammonium nutrition has been suggested to be associated with alterations in the oxidation-reduction state of leaf cells. Herein, we show that ammonium nutrition in Arabidopsis thaliana increases leaf NAD(P)H/NAD(P)(+) ratio, reactive oxygen species content and accumulation of biomolecules oxidized by free radicals. We used the method of rapid fractionation of protoplasts to analyse which cellular compartments were over-reduced under ammonium supply and revealed that observed changes in NAD(P)H/NAD(P)(+) ratio involved only the extrachloroplastic fraction. We also showed that ammonium nutrition changes mitochondrial electron transport chain activity, increasing mitochondrial reactive oxygen species production. Our results indicate that the... (More)
Ammonium nutrition has been suggested to be associated with alterations in the oxidation-reduction state of leaf cells. Herein, we show that ammonium nutrition in Arabidopsis thaliana increases leaf NAD(P)H/NAD(P)(+) ratio, reactive oxygen species content and accumulation of biomolecules oxidized by free radicals. We used the method of rapid fractionation of protoplasts to analyse which cellular compartments were over-reduced under ammonium supply and revealed that observed changes in NAD(P)H/NAD(P)(+) ratio involved only the extrachloroplastic fraction. We also showed that ammonium nutrition changes mitochondrial electron transport chain activity, increasing mitochondrial reactive oxygen species production. Our results indicate that the functional impairment associated with ammonium nutrition is mainly associated with redox reactions outside the chloroplast. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
chloroplasts, mitochondria, photosynthesis, redox homeostasis, reductive, stress, ROS metabolism
in
Plant, Cell and Environment
volume
36
issue
11
pages
2034 - 2045
publisher
Wiley-Blackwell
external identifiers
  • wos:000325227400012
  • scopus:84884975650
ISSN
0140-7791
DOI
10.1111/pce.12113
language
English
LU publication?
yes
id
02ab5eb8-c73a-47d1-82d1-ba6fa0cdbd8c (old id 4171795)
date added to LUP
2013-11-25 14:42:38
date last changed
2019-11-13 01:34:50
@article{02ab5eb8-c73a-47d1-82d1-ba6fa0cdbd8c,
  abstract     = {Ammonium nutrition has been suggested to be associated with alterations in the oxidation-reduction state of leaf cells. Herein, we show that ammonium nutrition in Arabidopsis thaliana increases leaf NAD(P)H/NAD(P)(+) ratio, reactive oxygen species content and accumulation of biomolecules oxidized by free radicals. We used the method of rapid fractionation of protoplasts to analyse which cellular compartments were over-reduced under ammonium supply and revealed that observed changes in NAD(P)H/NAD(P)(+) ratio involved only the extrachloroplastic fraction. We also showed that ammonium nutrition changes mitochondrial electron transport chain activity, increasing mitochondrial reactive oxygen species production. Our results indicate that the functional impairment associated with ammonium nutrition is mainly associated with redox reactions outside the chloroplast.},
  author       = {Podgorska, Anna and Gieczewska, Katarzyna and Lukawska-Kuzma, Katarzyna and Rasmusson, Allan and Gardestrom, Per and Szal, Bozena},
  issn         = {0140-7791},
  keyword      = {chloroplasts,mitochondria,photosynthesis,redox homeostasis,reductive,stress,ROS metabolism},
  language     = {eng},
  number       = {11},
  pages        = {2034--2045},
  publisher    = {Wiley-Blackwell},
  series       = {Plant, Cell and Environment},
  title        = {Long-term ammonium nutrition of Arabidopsis increases the extrachloroplastic NAD(P)H/NAD(P)(+) ratio and mitochondrial reactive oxygen species level in leaves but does not impair photosynthetic capacity},
  url          = {http://dx.doi.org/10.1111/pce.12113},
  volume       = {36},
  year         = {2013},
}