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Multiplex profiling of serum proteins in solution using barcoded antibody fragments and next generation sequencing

Brofelth, Mattias LU ; Ekstrand, Anna Isinger LU orcid ; Gour, Shashank ; Jansson, Ronnie ; Hedhammar, My ; Elleby, Björn ; Kvist, Anders LU ; Wingren, Christer LU ; Axelsson, Ulrika LU orcid and Borrebaeck, Carl A.K. LU (2020) In Communications Biology 3(1).
Abstract

The composition of serum proteins is reflecting the current health status and can, with the right tools, be used to detect early signs of disease, such as an emerging cancer. An earlier diagnosis of cancer would greatly increase the chance of an improved outcome for the patients. However, there is still an unmet need for proficient tools to decipher the information in the blood proteome, which calls for further technological development. Here, we present a proof-of-concept study that demonstrates an alternative approach for multiplexed protein profiling of serum samples in solution, using DNA barcoded scFv antibody fragments and next generation sequencing. The outcome shows high accuracy when discriminating samples derived from... (More)

The composition of serum proteins is reflecting the current health status and can, with the right tools, be used to detect early signs of disease, such as an emerging cancer. An earlier diagnosis of cancer would greatly increase the chance of an improved outcome for the patients. However, there is still an unmet need for proficient tools to decipher the information in the blood proteome, which calls for further technological development. Here, we present a proof-of-concept study that demonstrates an alternative approach for multiplexed protein profiling of serum samples in solution, using DNA barcoded scFv antibody fragments and next generation sequencing. The outcome shows high accuracy when discriminating samples derived from pancreatic cancer patients and healthy controls and represents a scalable alternative for serum analysis.

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author
; ; ; ; ; ; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Communications Biology
volume
3
issue
1
article number
339
publisher
Nature Publishing Group
external identifiers
  • scopus:85087390576
  • pmid:32620783
ISSN
2399-3642
DOI
10.1038/s42003-020-1068-0
language
English
LU publication?
yes
id
02bb3d21-4d9c-43f5-8bf3-019ed9659238
date added to LUP
2020-07-14 11:01:09
date last changed
2024-06-13 20:44:32
@article{02bb3d21-4d9c-43f5-8bf3-019ed9659238,
  abstract     = {{<p>The composition of serum proteins is reflecting the current health status and can, with the right tools, be used to detect early signs of disease, such as an emerging cancer. An earlier diagnosis of cancer would greatly increase the chance of an improved outcome for the patients. However, there is still an unmet need for proficient tools to decipher the information in the blood proteome, which calls for further technological development. Here, we present a proof-of-concept study that demonstrates an alternative approach for multiplexed protein profiling of serum samples in solution, using DNA barcoded scFv antibody fragments and next generation sequencing. The outcome shows high accuracy when discriminating samples derived from pancreatic cancer patients and healthy controls and represents a scalable alternative for serum analysis.</p>}},
  author       = {{Brofelth, Mattias and Ekstrand, Anna Isinger and Gour, Shashank and Jansson, Ronnie and Hedhammar, My and Elleby, Björn and Kvist, Anders and Wingren, Christer and Axelsson, Ulrika and Borrebaeck, Carl A.K.}},
  issn         = {{2399-3642}},
  language     = {{eng}},
  number       = {{1}},
  publisher    = {{Nature Publishing Group}},
  series       = {{Communications Biology}},
  title        = {{Multiplex profiling of serum proteins in solution using barcoded antibody fragments and next generation sequencing}},
  url          = {{http://dx.doi.org/10.1038/s42003-020-1068-0}},
  doi          = {{10.1038/s42003-020-1068-0}},
  volume       = {{3}},
  year         = {{2020}},
}