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Shape and Phase Transitions in a PEGylated Phospholipid System

Viitala, Lauri; Pajari, Saija; Gentile, Luigi LU ; Määttä, Jukka; Gubitosi, Marta LU ; Deska, Jan; Sammalkorpi, Maria; Olsson, Ulf LU and Murtomäki, Lasse (2019) In Langmuir 35(11). p.3999-4010
Abstract

Poly(ethylene glycol) (PEG) polymers and PEG-conjugated lipids are widely used in bioengineering and drug transport applications. A PEG layer in a drug carrier increases hydrophilic repulsion, inhibits membrane fusion and serum opsonin interactions, and prolongs the storage and circulation time. It can also change the carrier shape and have an influence on many properties related to the content release of the carrier. In this paper, we focus on the physicochemical effects of PEGylation in the lipid bilayer. We introduce laurdanC as a fluorophore for shape recognition and phase transition detection. Together with laurdanC, cryogenic transmission electron microscopy, differential scanning calorimetry, molecular dynamics simulations, and... (More)

Poly(ethylene glycol) (PEG) polymers and PEG-conjugated lipids are widely used in bioengineering and drug transport applications. A PEG layer in a drug carrier increases hydrophilic repulsion, inhibits membrane fusion and serum opsonin interactions, and prolongs the storage and circulation time. It can also change the carrier shape and have an influence on many properties related to the content release of the carrier. In this paper, we focus on the physicochemical effects of PEGylation in the lipid bilayer. We introduce laurdanC as a fluorophore for shape recognition and phase transition detection. Together with laurdanC, cryogenic transmission electron microscopy, differential scanning calorimetry, molecular dynamics simulations, and small-angle X-ray scattering/wide-angle X-ray scattering, we acquire information of the particle/bilayer morphology and phase behavior in systems containing 1,2-dipalmitoyl-sn-glycero-3-phosphocholine:1,2-distearoyl-sn-glycero-3-phosphoethanolamine-PEG(2000) with different fractions. We find that PEGylation leads to two important and potentially usable features of the system. (1) Spherical vesicles present a window of elevated chain-melting temperatures and (2) lipid packing shape-controlled liposome-to-bicelle transition. The first finding is significant for targets requiring multiple release sequences and the second enables tuning the release by composition and the PEG polymer length. Besides drug delivery systems, the findings can be used in other smart soft materials with trigger-polymers as well.

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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Langmuir
volume
35
issue
11
pages
3999 - 4010
publisher
The American Chemical Society
external identifiers
  • scopus:85062887743
ISSN
0743-7463
DOI
10.1021/acs.langmuir.8b03829
language
English
LU publication?
yes
id
02fc57b8-7e14-468f-92be-ca69273f93ab
date added to LUP
2019-03-21 14:23:47
date last changed
2019-04-23 04:47:15
@article{02fc57b8-7e14-468f-92be-ca69273f93ab,
  abstract     = {<p>Poly(ethylene glycol) (PEG) polymers and PEG-conjugated lipids are widely used in bioengineering and drug transport applications. A PEG layer in a drug carrier increases hydrophilic repulsion, inhibits membrane fusion and serum opsonin interactions, and prolongs the storage and circulation time. It can also change the carrier shape and have an influence on many properties related to the content release of the carrier. In this paper, we focus on the physicochemical effects of PEGylation in the lipid bilayer. We introduce laurdanC as a fluorophore for shape recognition and phase transition detection. Together with laurdanC, cryogenic transmission electron microscopy, differential scanning calorimetry, molecular dynamics simulations, and small-angle X-ray scattering/wide-angle X-ray scattering, we acquire information of the particle/bilayer morphology and phase behavior in systems containing 1,2-dipalmitoyl-sn-glycero-3-phosphocholine:1,2-distearoyl-sn-glycero-3-phosphoethanolamine-PEG(2000) with different fractions. We find that PEGylation leads to two important and potentially usable features of the system. (1) Spherical vesicles present a window of elevated chain-melting temperatures and (2) lipid packing shape-controlled liposome-to-bicelle transition. The first finding is significant for targets requiring multiple release sequences and the second enables tuning the release by composition and the PEG polymer length. Besides drug delivery systems, the findings can be used in other smart soft materials with trigger-polymers as well.</p>},
  author       = {Viitala, Lauri and Pajari, Saija and Gentile, Luigi and Määttä, Jukka and Gubitosi, Marta and Deska, Jan and Sammalkorpi, Maria and Olsson, Ulf and Murtomäki, Lasse},
  issn         = {0743-7463},
  language     = {eng},
  number       = {11},
  pages        = {3999--4010},
  publisher    = {The American Chemical Society},
  series       = {Langmuir},
  title        = {Shape and Phase Transitions in a PEGylated Phospholipid System},
  url          = {http://dx.doi.org/10.1021/acs.langmuir.8b03829},
  volume       = {35},
  year         = {2019},
}