Filter Plate–Based Screening of MIP SPE Materials for Capture of the Biomarker Pro-Gastrin-Releasing Peptide
(2017) In SLAS Discovery 22(10). p.1253-1261- Abstract
- Affinity-based solid-phase extraction (SPE) is an attractive low-cost sample preparation strategy for biomarker analysis. Molecularly imprinted polymers (MIPs) as affinity sorbents offer unique opportunities for affinity SPE, due to their low manufacturing cost and high robustness. A limitation is the prediction of their affinity; therefore, screening of analyte recovery and specificity within a large range of SPE conditions is important in order to ensure high-sensitivity detection and assay reproducibility. Here, a µ-SPE method for screening of the MIP-SPE materials using a commercial 384-well filter plate is presented. The method allows for rapid and automated screening using 10–30 µL of packed SPE sorbent per well and sample volumes in... (More)
- Affinity-based solid-phase extraction (SPE) is an attractive low-cost sample preparation strategy for biomarker analysis. Molecularly imprinted polymers (MIPs) as affinity sorbents offer unique opportunities for affinity SPE, due to their low manufacturing cost and high robustness. A limitation is the prediction of their affinity; therefore, screening of analyte recovery and specificity within a large range of SPE conditions is important in order to ensure high-sensitivity detection and assay reproducibility. Here, a µ-SPE method for screening of the MIP-SPE materials using a commercial 384-well filter plate is presented. The method allows for rapid and automated screening using 10–30 µL of packed SPE sorbent per well and sample volumes in the range of 10–70 µL. This enables screening of many different SPE sorbents while simultaneously identifying optimal SPE conditions. In addition, the 384-well format also facilitates detection with a multitude of analytical platforms. Performance of the µ-MIP-SPE method was investigated using a series of MIPs designed to capture pro-gastrin-releasing peptide (ProGRP). Fractions coming from sample load, cartridge wash, and elution were collected and analyzed using mass spectrometry (MS). The top-performing MIPs were identified, together with proper SPE conditions. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/03937050-c6f2-4d22-8b5d-542774588a39
- author
- Jagadeesan, Kishore LU ; Rossetti, Cecilia ; Abdel Qader, Abed ; Reubsaet, Léon ; Sellergren, Borje ; Laurell, Thomas LU and Ekström, Simon LU
- organization
- publishing date
- 2017-12-01
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Sample preparation, mass spectromery, SEPARATIONS, chromatography, protein chemistry, Biomarkers, protein labeling
- in
- SLAS Discovery
- volume
- 22
- issue
- 10
- pages
- 1253 - 1261
- publisher
- SAGE Publications
- external identifiers
-
- scopus:85029009662
- wos:000415922500010
- pmid:28346098
- ISSN
- 2472-5552
- DOI
- 10.1177/2472555216689494
- project
- Robust affinity materials for applications in proteomics and diagnostics
- language
- English
- LU publication?
- yes
- id
- 03937050-c6f2-4d22-8b5d-542774588a39
- date added to LUP
- 2017-02-11 21:43:24
- date last changed
- 2022-02-14 17:00:26
@article{03937050-c6f2-4d22-8b5d-542774588a39, abstract = {{Affinity-based solid-phase extraction (SPE) is an attractive low-cost sample preparation strategy for biomarker analysis. Molecularly imprinted polymers (MIPs) as affinity sorbents offer unique opportunities for affinity SPE, due to their low manufacturing cost and high robustness. A limitation is the prediction of their affinity; therefore, screening of analyte recovery and specificity within a large range of SPE conditions is important in order to ensure high-sensitivity detection and assay reproducibility. Here, a µ-SPE method for screening of the MIP-SPE materials using a commercial 384-well filter plate is presented. The method allows for rapid and automated screening using 10–30 µL of packed SPE sorbent per well and sample volumes in the range of 10–70 µL. This enables screening of many different SPE sorbents while simultaneously identifying optimal SPE conditions. In addition, the 384-well format also facilitates detection with a multitude of analytical platforms. Performance of the µ-MIP-SPE method was investigated using a series of MIPs designed to capture pro-gastrin-releasing peptide (ProGRP). Fractions coming from sample load, cartridge wash, and elution were collected and analyzed using mass spectrometry (MS). The top-performing MIPs were identified, together with proper SPE conditions.}}, author = {{Jagadeesan, Kishore and Rossetti, Cecilia and Abdel Qader, Abed and Reubsaet, Léon and Sellergren, Borje and Laurell, Thomas and Ekström, Simon}}, issn = {{2472-5552}}, keywords = {{Sample preparation; mass spectromery; SEPARATIONS; chromatography; protein chemistry; Biomarkers; protein labeling}}, language = {{eng}}, month = {{12}}, number = {{10}}, pages = {{1253--1261}}, publisher = {{SAGE Publications}}, series = {{SLAS Discovery}}, title = {{Filter Plate–Based Screening of MIP SPE Materials for Capture of the Biomarker Pro-Gastrin-Releasing Peptide}}, url = {{http://dx.doi.org/10.1177/2472555216689494}}, doi = {{10.1177/2472555216689494}}, volume = {{22}}, year = {{2017}}, }