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Filter Plate–Based Screening of MIP SPE Materials for Capture of the Biomarker Pro-Gastrin-Releasing Peptide

Jagadeesan, Kishore LU ; Rossetti, Cecilia; Abdel Qader, Abed; Reubsaet, Léon; Sellergren, Borje; Laurell, Thomas LU and Ekström, Simon LU (2017) In SLAS Discovery 22(10). p.1253-1261
Abstract
Affinity-based solid-phase extraction (SPE) is an attractive low-cost sample preparation strategy for biomarker analysis. Molecularly imprinted polymers (MIPs) as affinity sorbents offer unique opportunities for affinity SPE, due to their low manufacturing cost and high robustness. A limitation is the prediction of their affinity; therefore, screening of analyte recovery and specificity within a large range of SPE conditions is important in order to ensure high-sensitivity detection and assay reproducibility. Here, a µ-SPE method for screening of the MIP-SPE materials using a commercial 384-well filter plate is presented. The method allows for rapid and automated screening using 10–30 µL of packed SPE sorbent per well and sample volumes in... (More)
Affinity-based solid-phase extraction (SPE) is an attractive low-cost sample preparation strategy for biomarker analysis. Molecularly imprinted polymers (MIPs) as affinity sorbents offer unique opportunities for affinity SPE, due to their low manufacturing cost and high robustness. A limitation is the prediction of their affinity; therefore, screening of analyte recovery and specificity within a large range of SPE conditions is important in order to ensure high-sensitivity detection and assay reproducibility. Here, a µ-SPE method for screening of the MIP-SPE materials using a commercial 384-well filter plate is presented. The method allows for rapid and automated screening using 10–30 µL of packed SPE sorbent per well and sample volumes in the range of 10–70 µL. This enables screening of many different SPE sorbents while simultaneously identifying optimal SPE conditions. In addition, the 384-well format also facilitates detection with a multitude of analytical platforms. Performance of the µ-MIP-SPE method was investigated using a series of MIPs designed to capture pro-gastrin-releasing peptide (ProGRP). Fractions coming from sample load, cartridge wash, and elution were collected and analyzed using mass spectrometry (MS). The top-performing MIPs were identified, together with proper SPE conditions. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Sample preparation, mass spectromery, SEPARATIONS, chromatography, protein chemistry, Biomarkers, protein labeling
in
SLAS Discovery
volume
22
issue
10
pages
1253 - 1261
publisher
SAGE Publications Inc.
external identifiers
  • scopus:85029009662
  • wos:000415922500010
ISSN
2472-5552
DOI
10.1177/2472555216689494
language
English
LU publication?
yes
id
03937050-c6f2-4d22-8b5d-542774588a39
date added to LUP
2017-02-11 21:43:24
date last changed
2018-01-16 13:23:24
@article{03937050-c6f2-4d22-8b5d-542774588a39,
  abstract     = {Affinity-based solid-phase extraction (SPE) is an attractive low-cost sample preparation strategy for biomarker analysis. Molecularly imprinted polymers (MIPs) as affinity sorbents offer unique opportunities for affinity SPE, due to their low manufacturing cost and high robustness. A limitation is the prediction of their affinity; therefore, screening of analyte recovery and specificity within a large range of SPE conditions is important in order to ensure high-sensitivity detection and assay reproducibility. Here, a µ-SPE method for screening of the MIP-SPE materials using a commercial 384-well filter plate is presented. The method allows for rapid and automated screening using 10–30 µL of packed SPE sorbent per well and sample volumes in the range of 10–70 µL. This enables screening of many different SPE sorbents while simultaneously identifying optimal SPE conditions. In addition, the 384-well format also facilitates detection with a multitude of analytical platforms. Performance of the µ-MIP-SPE method was investigated using a series of MIPs designed to capture pro-gastrin-releasing peptide (ProGRP). Fractions coming from sample load, cartridge wash, and elution were collected and analyzed using mass spectrometry (MS). The top-performing MIPs were identified, together with proper SPE conditions.},
  author       = {Jagadeesan, Kishore and Rossetti, Cecilia and Abdel Qader, Abed and Reubsaet, Léon and Sellergren, Borje and Laurell, Thomas and Ekström, Simon},
  issn         = {2472-5552},
  keyword      = {Sample preparation,mass spectromery,SEPARATIONS,chromatography,protein chemistry,Biomarkers,protein labeling},
  language     = {eng},
  month        = {12},
  number       = {10},
  pages        = {1253--1261},
  publisher    = {SAGE Publications Inc.},
  series       = {SLAS Discovery},
  title        = {Filter Plate–Based Screening of MIP SPE Materials for Capture of the Biomarker Pro-Gastrin-Releasing Peptide},
  url          = {http://dx.doi.org/10.1177/2472555216689494},
  volume       = {22},
  year         = {2017},
}