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Long DNA isolation and imaging using lateral displacement arrays integrated with DNA combing

Ström, Oskar E. LU orcid ; Beech, Jason P. LU and Tegenfeldt, Jonas O. LU orcid (2019) 23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019 In 23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019 p.771-772
Abstract

We demonstrate proof of principle of an efficient and simple method combining sample preparation and stretching of DNA in a single device that can be used as basis for an integrated portable system for optical DNA mapping. An important bottleneck for DNA mapping, as well as for other methods, is the isolation of long DNA molecules from a background of contaminants such as shorter DNA fragments, dyes, and lysate. We demonstrate the isolation of longer (48.5 kbp or 166 kbp) from shorter (<10 kbp) DNA strands using Deterministic Lateral Displacement (DLD), followed by surface-stretching of the sorted DNA for mapping.

Please use this url to cite or link to this publication:
author
; and
organization
publishing date
type
Chapter in Book/Report/Conference proceeding
publication status
published
subject
keywords
DLD, DNA sample preparation, DNA Separation, DNA stretching, Molecular Combing
host publication
23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019
series title
23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019
pages
2 pages
publisher
Chemical and Biological Microsystems Society
conference name
23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019
conference location
Basel, Switzerland
conference dates
2019-10-27 - 2019-10-31
external identifiers
  • scopus:85094977467
ISBN
9781733419000
project
Microfluidic Preparation and Transport of Long DNA using Pillar Arrays
language
English
LU publication?
yes
id
03ad2ee8-753d-4a34-b919-0d024653f5c5
date added to LUP
2020-11-24 08:02:18
date last changed
2023-10-22 20:50:02
@inproceedings{03ad2ee8-753d-4a34-b919-0d024653f5c5,
  abstract     = {{<p>We demonstrate proof of principle of an efficient and simple method combining sample preparation and stretching of DNA in a single device that can be used as basis for an integrated portable system for optical DNA mapping. An important bottleneck for DNA mapping, as well as for other methods, is the isolation of long DNA molecules from a background of contaminants such as shorter DNA fragments, dyes, and lysate. We demonstrate the isolation of longer (48.5 kbp or 166 kbp) from shorter (&lt;10 kbp) DNA strands using Deterministic Lateral Displacement (DLD), followed by surface-stretching of the sorted DNA for mapping.</p>}},
  author       = {{Ström, Oskar E. and Beech, Jason P. and Tegenfeldt, Jonas O.}},
  booktitle    = {{23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019}},
  isbn         = {{9781733419000}},
  keywords     = {{DLD; DNA sample preparation; DNA Separation; DNA stretching; Molecular Combing}},
  language     = {{eng}},
  pages        = {{771--772}},
  publisher    = {{Chemical and Biological Microsystems Society}},
  series       = {{23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019}},
  title        = {{Long DNA isolation and imaging using lateral displacement arrays integrated with DNA combing}},
  year         = {{2019}},
}