Skip to main content

Lund University Publications

LUND UNIVERSITY LIBRARIES

Enzyme-linked immunosorbent assay using thin-layered microfluidics with perfect capture of the target protein

Smirnova, Adelina ; Ohta, Ryoichi ; Mori, Emi ; Shimizu, Hisashi ; Morikawa, Kyojiro and Kitamori, Takehiko LU (2023) In Analytical Methods 15(5). p.675-684
Abstract

We developed a process for enzyme-linked immunosorbent assay on a glass microchip via the use of a thin-layered microfluidic channel. This channel possesses a high aspect ratio (width/depth ∼200) and has an antibody layer immobilized directly on the channel surface. A depth of several microns and an excessive width and length (mm scale) of the channel provide a large-volume capacity (102 nL) and maximum capture efficiency of the analyte for a high level of detection sensitivity (102 pg mL−1). The developed reusable immunosensor has demonstrated high-performance characteristics by requiring less than 50 μL of sample and providing analysis in less than 25 min. This new method could impact the development... (More)

We developed a process for enzyme-linked immunosorbent assay on a glass microchip via the use of a thin-layered microfluidic channel. This channel possesses a high aspect ratio (width/depth ∼200) and has an antibody layer immobilized directly on the channel surface. A depth of several microns and an excessive width and length (mm scale) of the channel provide a large-volume capacity (102 nL) and maximum capture efficiency of the analyte for a high level of detection sensitivity (102 pg mL−1). The developed reusable immunosensor has demonstrated high-performance characteristics by requiring less than 50 μL of sample and providing analysis in less than 25 min. This new method could impact the development of point-of-care devices for biomedical applications.

(Less)
Please use this url to cite or link to this publication:
author
; ; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Analytical Methods
volume
15
issue
5
pages
10 pages
publisher
Royal Society of Chemistry
external identifiers
  • pmid:36655604
  • scopus:85146905373
ISSN
1759-9660
DOI
10.1039/d2ay01686a
language
English
LU publication?
yes
id
03cb858d-3650-49a9-9825-a60f8d539687
date added to LUP
2023-02-14 15:15:00
date last changed
2024-04-04 16:33:13
@article{03cb858d-3650-49a9-9825-a60f8d539687,
  abstract     = {{<p>We developed a process for enzyme-linked immunosorbent assay on a glass microchip via the use of a thin-layered microfluidic channel. This channel possesses a high aspect ratio (width/depth ∼200) and has an antibody layer immobilized directly on the channel surface. A depth of several microns and an excessive width and length (mm scale) of the channel provide a large-volume capacity (10<sup>2</sup> nL) and maximum capture efficiency of the analyte for a high level of detection sensitivity (10<sup>2</sup> pg mL<sup>−1</sup>). The developed reusable immunosensor has demonstrated high-performance characteristics by requiring less than 50 μL of sample and providing analysis in less than 25 min. This new method could impact the development of point-of-care devices for biomedical applications.</p>}},
  author       = {{Smirnova, Adelina and Ohta, Ryoichi and Mori, Emi and Shimizu, Hisashi and Morikawa, Kyojiro and Kitamori, Takehiko}},
  issn         = {{1759-9660}},
  language     = {{eng}},
  number       = {{5}},
  pages        = {{675--684}},
  publisher    = {{Royal Society of Chemistry}},
  series       = {{Analytical Methods}},
  title        = {{Enzyme-linked immunosorbent assay using thin-layered microfluidics with perfect capture of the target protein}},
  url          = {{http://dx.doi.org/10.1039/d2ay01686a}},
  doi          = {{10.1039/d2ay01686a}},
  volume       = {{15}},
  year         = {{2023}},
}