The hormone-sensitive lipase (LIPE) gene located on chromosome 19q13.1-->13.2 is not duplicated on 19p13.3
(1995) In International Journal of Obesity 19(8). p.590-592- Abstract
- The existence of a DNA polymorphism at the hormone-sensitive lipase locus could be of great interest for genetic analysis of obesity and related disorders since hormone-sensitive lipase is the rate-limiting enzyme of adipose tissue lipolysis and therefore plays a key role in energy metabolism. The polymorphic dinucleotide repeat D19S120 was identified within a human genomic clone selected with a rat hormone-sensitive lipase cDNA. This marker was subsequently localized to the short arm of chromosome 19 (p13.3) whereas human hormone-sensitive lipase (LIPE) had been mapped to the long arm of chromosome 19 (q13.1-->13.2). A duplication of the hormone-sensitive lipase gene or the presence of a pseudogene could explain the discrepancy.... (More)
- The existence of a DNA polymorphism at the hormone-sensitive lipase locus could be of great interest for genetic analysis of obesity and related disorders since hormone-sensitive lipase is the rate-limiting enzyme of adipose tissue lipolysis and therefore plays a key role in energy metabolism. The polymorphic dinucleotide repeat D19S120 was identified within a human genomic clone selected with a rat hormone-sensitive lipase cDNA. This marker was subsequently localized to the short arm of chromosome 19 (p13.3) whereas human hormone-sensitive lipase (LIPE) had been mapped to the long arm of chromosome 19 (q13.1-->13.2). A duplication of the hormone-sensitive lipase gene or the presence of a pseudogene could explain the discrepancy. Cosmids from the two regions were analyzed in Southern blot experiments. A human adipose tissue hormone-sensitive lipase full-length cDNA probe hybridized only to cosmids from the 19q13.1-->13.2 region whereas the D19S120 amplicon probe hybridized only to cosmids from the p13.3 region. These data show that the occurrence of gene duplication or the presence of a pseudogene on the short arm of chromosome 19 is very unlikely and that D19S120 is unrelated to the hormone-sensitive lipase gene. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1108772
- author
- Laurell, H ; Grober, J ; Stenson, Lena LU ; Holm, C ; Mohrenweiser, H W and Langin, D
- organization
- publishing date
- 1995
- type
- Contribution to journal
- publication status
- published
- subject
- in
- International Journal of Obesity
- volume
- 19
- issue
- 8
- pages
- 590 - 592
- publisher
- Nature Publishing Group
- external identifiers
-
- pmid:7489032
- scopus:0029066204
- ISSN
- 1476-5497
- language
- English
- LU publication?
- yes
- id
- 045a4956-1507-4df7-af6a-88e3e26d09bb (old id 1108772)
- date added to LUP
- 2016-04-01 12:35:16
- date last changed
- 2021-01-03 06:10:44
@article{045a4956-1507-4df7-af6a-88e3e26d09bb,
abstract = {{The existence of a DNA polymorphism at the hormone-sensitive lipase locus could be of great interest for genetic analysis of obesity and related disorders since hormone-sensitive lipase is the rate-limiting enzyme of adipose tissue lipolysis and therefore plays a key role in energy metabolism. The polymorphic dinucleotide repeat D19S120 was identified within a human genomic clone selected with a rat hormone-sensitive lipase cDNA. This marker was subsequently localized to the short arm of chromosome 19 (p13.3) whereas human hormone-sensitive lipase (LIPE) had been mapped to the long arm of chromosome 19 (q13.1-->13.2). A duplication of the hormone-sensitive lipase gene or the presence of a pseudogene could explain the discrepancy. Cosmids from the two regions were analyzed in Southern blot experiments. A human adipose tissue hormone-sensitive lipase full-length cDNA probe hybridized only to cosmids from the 19q13.1-->13.2 region whereas the D19S120 amplicon probe hybridized only to cosmids from the p13.3 region. These data show that the occurrence of gene duplication or the presence of a pseudogene on the short arm of chromosome 19 is very unlikely and that D19S120 is unrelated to the hormone-sensitive lipase gene.}},
author = {{Laurell, H and Grober, J and Stenson, Lena and Holm, C and Mohrenweiser, H W and Langin, D}},
issn = {{1476-5497}},
language = {{eng}},
number = {{8}},
pages = {{590--592}},
publisher = {{Nature Publishing Group}},
series = {{International Journal of Obesity}},
title = {{The hormone-sensitive lipase (LIPE) gene located on chromosome 19q13.1-->13.2 is not duplicated on 19p13.3}},
volume = {{19}},
year = {{1995}},
}