Low reaction temperature increases the selectivity in an enzymatic reaction due to substrate solvation effects
(1997) In Biotechnology Letters 19(1). p.85-88- Abstract
Immobilized α-chymotrypsin was used as catalyst for studying temperature effects on acyl transfer reactions (acyl-donor: Bz-TyrOEt) in a water-immiscible organic solvent. The solubility of the two nucleophiles, Phe-NH2 and water, decreased with decreasing temperature. The relative decrease for the amide was larger (2.4-fold) than for water. Therefore the thermodynamic activity (estimated by the relative saturation) increased more for this substrate and hence the selectivity in the reaction was increased.
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https://lup.lub.lu.se/record/053cf2ad-f498-4b16-9077-145273f3b109
- author
- Jönsson, Åsa LU ; Wehtje, Ernst LU and Adlercreutz, Patrick LU
- organization
- publishing date
- 1997-03-08
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Biotechnology Letters
- volume
- 19
- issue
- 1
- pages
- 4 pages
- publisher
- Springer
- external identifiers
-
- scopus:0031042081
- ISSN
- 0141-5492
- DOI
- 10.1023/A:1018379423480
- language
- English
- LU publication?
- yes
- id
- 053cf2ad-f498-4b16-9077-145273f3b109
- date added to LUP
- 2019-06-20 16:32:40
- date last changed
- 2022-01-31 22:13:27
@article{053cf2ad-f498-4b16-9077-145273f3b109, abstract = {{<p>Immobilized α-chymotrypsin was used as catalyst for studying temperature effects on acyl transfer reactions (acyl-donor: Bz-TyrOEt) in a water-immiscible organic solvent. The solubility of the two nucleophiles, Phe-NH<sub>2</sub> and water, decreased with decreasing temperature. The relative decrease for the amide was larger (2.4-fold) than for water. Therefore the thermodynamic activity (estimated by the relative saturation) increased more for this substrate and hence the selectivity in the reaction was increased.</p>}}, author = {{Jönsson, Åsa and Wehtje, Ernst and Adlercreutz, Patrick}}, issn = {{0141-5492}}, language = {{eng}}, month = {{03}}, number = {{1}}, pages = {{85--88}}, publisher = {{Springer}}, series = {{Biotechnology Letters}}, title = {{Low reaction temperature increases the selectivity in an enzymatic reaction due to substrate solvation effects}}, url = {{http://dx.doi.org/10.1023/A:1018379423480}}, doi = {{10.1023/A:1018379423480}}, volume = {{19}}, year = {{1997}}, }