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Low reaction temperature increases the selectivity in an enzymatic reaction due to substrate solvation effects

Jönsson, Åsa LU ; Wehtje, Ernst LU and Adlercreutz, Patrick LU orcid (1997) In Biotechnology Letters 19(1). p.85-88
Abstract

Immobilized α-chymotrypsin was used as catalyst for studying temperature effects on acyl transfer reactions (acyl-donor: Bz-TyrOEt) in a water-immiscible organic solvent. The solubility of the two nucleophiles, Phe-NH2 and water, decreased with decreasing temperature. The relative decrease for the amide was larger (2.4-fold) than for water. Therefore the thermodynamic activity (estimated by the relative saturation) increased more for this substrate and hence the selectivity in the reaction was increased.

Please use this url to cite or link to this publication:
author
; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Biotechnology Letters
volume
19
issue
1
pages
4 pages
publisher
Springer
external identifiers
  • scopus:0031042081
ISSN
0141-5492
DOI
10.1023/A:1018379423480
language
English
LU publication?
yes
id
053cf2ad-f498-4b16-9077-145273f3b109
date added to LUP
2019-06-20 16:32:40
date last changed
2022-01-31 22:13:27
@article{053cf2ad-f498-4b16-9077-145273f3b109,
  abstract     = {{<p>Immobilized α-chymotrypsin was used as catalyst for studying temperature effects on acyl transfer reactions (acyl-donor: Bz-TyrOEt) in a water-immiscible organic solvent. The solubility of the two nucleophiles, Phe-NH<sub>2</sub> and water, decreased with decreasing temperature. The relative decrease for the amide was larger (2.4-fold) than for water. Therefore the thermodynamic activity (estimated by the relative saturation) increased more for this substrate and hence the selectivity in the reaction was increased.</p>}},
  author       = {{Jönsson, Åsa and Wehtje, Ernst and Adlercreutz, Patrick}},
  issn         = {{0141-5492}},
  language     = {{eng}},
  month        = {{03}},
  number       = {{1}},
  pages        = {{85--88}},
  publisher    = {{Springer}},
  series       = {{Biotechnology Letters}},
  title        = {{Low reaction temperature increases the selectivity in an enzymatic reaction due to substrate solvation effects}},
  url          = {{http://dx.doi.org/10.1023/A:1018379423480}},
  doi          = {{10.1023/A:1018379423480}},
  volume       = {{19}},
  year         = {{1997}},
}