EndoSd : An IgG glycan hydrolyzing enzyme in Streptococcus dysgalactiae subspecies dysgalactiae
(2016) In Future Microbiology 11(6). p.721-736- Abstract
Aim: The aim of this study was to identify and characterize EndoS-like enzymes in Streptococcus dysgalactiae subspecies dysgalactiae (SDSD). Materials & methods: PCR, DNA sequencing, recombinant protein expression, lectin blot, ultra high performance liquid chromatography analysis and a chitinase assay were used to identify ndoS-like genes and characterize EndoSd. Results: EndoSd were found in four SDSD strains. EndoSd hydrolyzes the chitobiose core of the glycan on IgG. The amino acid sequence of EndoSd is 70% identical to EndoS in S. pyogenes, but it has a unique C-terminal sequence. EndoSd secretion is influenced by the carbohydrate composition of the growth medium. Conclusion: Our findings indicate that IgG glycan hydrolyzing... (More)
Aim: The aim of this study was to identify and characterize EndoS-like enzymes in Streptococcus dysgalactiae subspecies dysgalactiae (SDSD). Materials & methods: PCR, DNA sequencing, recombinant protein expression, lectin blot, ultra high performance liquid chromatography analysis and a chitinase assay were used to identify ndoS-like genes and characterize EndoSd. Results: EndoSd were found in four SDSD strains. EndoSd hydrolyzes the chitobiose core of the glycan on IgG. The amino acid sequence of EndoSd is 70% identical to EndoS in S. pyogenes, but it has a unique C-terminal sequence. EndoSd secretion is influenced by the carbohydrate composition of the growth medium. Conclusion: Our findings indicate that IgG glycan hydrolyzing activity is present in SDSD, and that the activity can be attributed to the here identified enzyme EndoSd.
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- author
- Shadnezhad, Azadeh
LU
; Nägeli, Andreas
LU
; Sjögren, Jonathan
LU
; Adamczyk, Barbara
; Leo, Fredrik
; Allhorn, Maria
LU
; Karlsson, Niclas G.
; Jensen, Anders
and Collin, Mattias
LU
- organization
- publishing date
- 2016-06-01
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- asparagine linked, chitinases, Endo-β-N-acetylglucosaminidase, glycosidase, IgG, Streptococcus dysgalactiae
- in
- Future Microbiology
- volume
- 11
- issue
- 6
- pages
- 16 pages
- publisher
- Future Medicine Ltd.
- external identifiers
-
- scopus:84971569538
- pmid:27200457
- wos:000376677500004
- ISSN
- 1746-0913
- DOI
- 10.2217/fmb.16.14
- language
- English
- LU publication?
- yes
- id
- 0547962c-b528-4307-8ce7-fe79bc3e1cd4
- date added to LUP
- 2016-06-16 12:48:33
- date last changed
- 2025-01-12 07:22:23
@article{0547962c-b528-4307-8ce7-fe79bc3e1cd4, abstract = {{<p>Aim: The aim of this study was to identify and characterize EndoS-like enzymes in Streptococcus dysgalactiae subspecies dysgalactiae (SDSD). Materials & methods: PCR, DNA sequencing, recombinant protein expression, lectin blot, ultra high performance liquid chromatography analysis and a chitinase assay were used to identify ndoS-like genes and characterize EndoSd. Results: EndoSd were found in four SDSD strains. EndoSd hydrolyzes the chitobiose core of the glycan on IgG. The amino acid sequence of EndoSd is 70% identical to EndoS in S. pyogenes, but it has a unique C-terminal sequence. EndoSd secretion is influenced by the carbohydrate composition of the growth medium. Conclusion: Our findings indicate that IgG glycan hydrolyzing activity is present in SDSD, and that the activity can be attributed to the here identified enzyme EndoSd.</p>}}, author = {{Shadnezhad, Azadeh and Nägeli, Andreas and Sjögren, Jonathan and Adamczyk, Barbara and Leo, Fredrik and Allhorn, Maria and Karlsson, Niclas G. and Jensen, Anders and Collin, Mattias}}, issn = {{1746-0913}}, keywords = {{asparagine linked; chitinases; Endo-β-N-acetylglucosaminidase; glycosidase; IgG; Streptococcus dysgalactiae}}, language = {{eng}}, month = {{06}}, number = {{6}}, pages = {{721--736}}, publisher = {{Future Medicine Ltd.}}, series = {{Future Microbiology}}, title = {{EndoSd : An IgG glycan hydrolyzing enzyme in Streptococcus dysgalactiae subspecies dysgalactiae}}, url = {{http://dx.doi.org/10.2217/fmb.16.14}}, doi = {{10.2217/fmb.16.14}}, volume = {{11}}, year = {{2016}}, }