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EndoSd : An IgG glycan hydrolyzing enzyme in Streptococcus dysgalactiae subspecies dysgalactiae

Shadnezhad, Azadeh LU ; Nägeli, Andreas LU ; Sjögren, Jonathan LU ; Adamczyk, Barbara ; Leo, Fredrik ; Allhorn, Maria LU ; Karlsson, Niclas G. ; Jensen, Anders and Collin, Mattias LU orcid (2016) In Future Microbiology 11(6). p.721-736
Abstract

Aim: The aim of this study was to identify and characterize EndoS-like enzymes in Streptococcus dysgalactiae subspecies dysgalactiae (SDSD). Materials & methods: PCR, DNA sequencing, recombinant protein expression, lectin blot, ultra high performance liquid chromatography analysis and a chitinase assay were used to identify ndoS-like genes and characterize EndoSd. Results: EndoSd were found in four SDSD strains. EndoSd hydrolyzes the chitobiose core of the glycan on IgG. The amino acid sequence of EndoSd is 70% identical to EndoS in S. pyogenes, but it has a unique C-terminal sequence. EndoSd secretion is influenced by the carbohydrate composition of the growth medium. Conclusion: Our findings indicate that IgG glycan hydrolyzing... (More)

Aim: The aim of this study was to identify and characterize EndoS-like enzymes in Streptococcus dysgalactiae subspecies dysgalactiae (SDSD). Materials & methods: PCR, DNA sequencing, recombinant protein expression, lectin blot, ultra high performance liquid chromatography analysis and a chitinase assay were used to identify ndoS-like genes and characterize EndoSd. Results: EndoSd were found in four SDSD strains. EndoSd hydrolyzes the chitobiose core of the glycan on IgG. The amino acid sequence of EndoSd is 70% identical to EndoS in S. pyogenes, but it has a unique C-terminal sequence. EndoSd secretion is influenced by the carbohydrate composition of the growth medium. Conclusion: Our findings indicate that IgG glycan hydrolyzing activity is present in SDSD, and that the activity can be attributed to the here identified enzyme EndoSd.

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author
; ; ; ; ; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
asparagine linked, chitinases, Endo-β-N-acetylglucosaminidase, glycosidase, IgG, Streptococcus dysgalactiae
in
Future Microbiology
volume
11
issue
6
pages
16 pages
publisher
Future Medicine Ltd.
external identifiers
  • pmid:27200457
  • wos:000376677500004
  • scopus:84971569538
ISSN
1746-0913
DOI
10.2217/fmb.16.14
language
English
LU publication?
yes
id
0547962c-b528-4307-8ce7-fe79bc3e1cd4
date added to LUP
2016-06-16 12:48:33
date last changed
2025-10-14 10:41:11
@article{0547962c-b528-4307-8ce7-fe79bc3e1cd4,
  abstract     = {{<p>Aim: The aim of this study was to identify and characterize EndoS-like enzymes in Streptococcus dysgalactiae subspecies dysgalactiae (SDSD). Materials &amp; methods: PCR, DNA sequencing, recombinant protein expression, lectin blot, ultra high performance liquid chromatography analysis and a chitinase assay were used to identify ndoS-like genes and characterize EndoSd. Results: EndoSd were found in four SDSD strains. EndoSd hydrolyzes the chitobiose core of the glycan on IgG. The amino acid sequence of EndoSd is 70% identical to EndoS in S. pyogenes, but it has a unique C-terminal sequence. EndoSd secretion is influenced by the carbohydrate composition of the growth medium. Conclusion: Our findings indicate that IgG glycan hydrolyzing activity is present in SDSD, and that the activity can be attributed to the here identified enzyme EndoSd.</p>}},
  author       = {{Shadnezhad, Azadeh and Nägeli, Andreas and Sjögren, Jonathan and Adamczyk, Barbara and Leo, Fredrik and Allhorn, Maria and Karlsson, Niclas G. and Jensen, Anders and Collin, Mattias}},
  issn         = {{1746-0913}},
  keywords     = {{asparagine linked; chitinases; Endo-β-N-acetylglucosaminidase; glycosidase; IgG; Streptococcus dysgalactiae}},
  language     = {{eng}},
  month        = {{06}},
  number       = {{6}},
  pages        = {{721--736}},
  publisher    = {{Future Medicine Ltd.}},
  series       = {{Future Microbiology}},
  title        = {{EndoSd : An IgG glycan hydrolyzing enzyme in Streptococcus dysgalactiae subspecies dysgalactiae}},
  url          = {{http://dx.doi.org/10.2217/fmb.16.14}},
  doi          = {{10.2217/fmb.16.14}},
  volume       = {{11}},
  year         = {{2016}},
}