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Phospholipase A2 activity is required for regeneration of sensory axons in cultured adult sciatic nerves

Edström, A. LU ; Briggman, M. and Ekström, Per LU (1996) In Journal of Neuroscience Research 43(2). p.183-189
Abstract

The adult frog dorsal root ganglia (DRGs) and their sciatic nerves (ScN) survive in organ culture for several days. About 3 days after a local test crush, the sensory axons start to regenerate into the distal nerve stump at a rate of approximately 0.6-0.9 mm/day. The axonal outgrowth is inhibited in a non-toxic way by low concentrations of three different phospholipase A2 (PLA2) inhibitors: 4-bromophenacyl bromide (BPB), aristolochic acid, and oleyl-oxyethyl-phosphoryl-choline (OOPC). In contrast, the outgrowth was slightly stimulated by 0.2 μM melittin, a PLA2 activator. Most experiments refer to the effects of BPB, which was shown to almost completely inhibit outgrowth at a concentration which did not affect... (More)

The adult frog dorsal root ganglia (DRGs) and their sciatic nerves (ScN) survive in organ culture for several days. About 3 days after a local test crush, the sensory axons start to regenerate into the distal nerve stump at a rate of approximately 0.6-0.9 mm/day. The axonal outgrowth is inhibited in a non-toxic way by low concentrations of three different phospholipase A2 (PLA2) inhibitors: 4-bromophenacyl bromide (BPB), aristolochic acid, and oleyl-oxyethyl-phosphoryl-choline (OOPC). In contrast, the outgrowth was slightly stimulated by 0.2 μM melittin, a PLA2 activator. Most experiments refer to the effects of BPB, which was shown to almost completely inhibit outgrowth at a concentration which did not affect either ganglionic protein synthesis or axonal transport. Using a compartmental system it could clearly be shown that BPB exerted its action in the outgrowth region. Other experiments showed that the initial period (about 3 days), which precedes the outgrowth, was unaffected by BPB. Several structures, including axonal ones, showed immunoreactivity for the low molecular form of PLA2 (sPLA2). The results suggest that PLA2 activity plays an important role in nerve regeneration and exerts its action at a local level, where the growth cones move forward.

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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
4-bromophenacyl bromide, melittin, phospholipase A2, regeneration, sciatic nerve
in
Journal of Neuroscience Research
volume
43
issue
2
pages
7 pages
publisher
John Wiley & Sons
external identifiers
  • Scopus:0030023222
ISSN
0360-4012
DOI
10.1002/(SICI)1097-4547(19960115)43:2<183::AID-JNR6>3.0.CO;2-C
language
English
LU publication?
yes
id
05d02dfe-46f7-4d19-83dd-84a3f7367037
date added to LUP
2016-12-07 14:55:55
date last changed
2017-01-25 16:26:58
@article{05d02dfe-46f7-4d19-83dd-84a3f7367037,
  abstract     = {<p>The adult frog dorsal root ganglia (DRGs) and their sciatic nerves (ScN) survive in organ culture for several days. About 3 days after a local test crush, the sensory axons start to regenerate into the distal nerve stump at a rate of approximately 0.6-0.9 mm/day. The axonal outgrowth is inhibited in a non-toxic way by low concentrations of three different phospholipase A2 (PLA<sub>2</sub>) inhibitors: 4-bromophenacyl bromide (BPB), aristolochic acid, and oleyl-oxyethyl-phosphoryl-choline (OOPC). In contrast, the outgrowth was slightly stimulated by 0.2 μM melittin, a PLA<sub>2</sub> activator. Most experiments refer to the effects of BPB, which was shown to almost completely inhibit outgrowth at a concentration which did not affect either ganglionic protein synthesis or axonal transport. Using a compartmental system it could clearly be shown that BPB exerted its action in the outgrowth region. Other experiments showed that the initial period (about 3 days), which precedes the outgrowth, was unaffected by BPB. Several structures, including axonal ones, showed immunoreactivity for the low molecular form of PLA<sub>2</sub> (sPLA<sub>2</sub>). The results suggest that PLA<sub>2</sub> activity plays an important role in nerve regeneration and exerts its action at a local level, where the growth cones move forward.</p>},
  author       = {Edström, A. and Briggman, M. and Ekström, Per},
  issn         = {0360-4012},
  keyword      = {4-bromophenacyl bromide,melittin,phospholipase A2,regeneration,sciatic nerve},
  language     = {eng},
  number       = {2},
  pages        = {183--189},
  publisher    = {John Wiley & Sons},
  series       = {Journal of Neuroscience Research},
  title        = {Phospholipase A2 activity is required for regeneration of sensory axons in cultured adult sciatic nerves},
  url          = {http://dx.doi.org/10.1002/(SICI)1097-4547(19960115)43:2<183::AID-JNR6>3.0.CO;2-C},
  volume       = {43},
  year         = {1996},
}