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Aeration rate effect on the growth kinetics, phytase production and plasmid stability of recombinant Escherichia coli BL21 (DE3)

Othman, Nor Zalina ; Elsayed, Elsayed A. ; Malek, Roslinda Abd ; Ramli, Solleh ; Masri, Helmy J. ; Sarmidi, Mohamed R. ; Aziz, Ramlan ; Wadaan, Mohamad A. ; Hatti-Kaul, Rajni LU and El Enshasy, Hesham A. (2014) In Journal of Pure and Applied Microbiology 8(4). p.2721-2728
Abstract

Phytase production using Escherichia coli BL21 (DE3), a recombinant strain harboring a plasmid encoding thermostable Bacillus phytase, in semi-industrial scale was studied in this work. Among the factors needed to be considered in order to achieve high enzyme yield, aeration rate plays an important role. Suitable aeration is required to supply cells with sufficient amount of air for cell and phytase production. The effect of different aeration rates, (1.0, 2.0 and 3.0 vv-1min-1), on the kinetics of cell growth and phytase production by the recombinant E. coli BL21 (DE3) in 16-L pilot scale stirred tank bioreactor was investigated. The highest cell concentration of 3.81 gL-1 concomitant with the maximal... (More)

Phytase production using Escherichia coli BL21 (DE3), a recombinant strain harboring a plasmid encoding thermostable Bacillus phytase, in semi-industrial scale was studied in this work. Among the factors needed to be considered in order to achieve high enzyme yield, aeration rate plays an important role. Suitable aeration is required to supply cells with sufficient amount of air for cell and phytase production. The effect of different aeration rates, (1.0, 2.0 and 3.0 vv-1min-1), on the kinetics of cell growth and phytase production by the recombinant E. coli BL21 (DE3) in 16-L pilot scale stirred tank bioreactor was investigated. The highest cell concentration of 3.81 gL-1 concomitant with the maximal total phytase production of 15.63 UmL-1 were obtained in a bioreactor of aeration rate 3.0vv-1min-1. At this high aeration rate, a maximal specific growth rate (μmax) and the maximal specific phytase production of 0.33 h-1 and 4102Ug-1, respectively, were achieved.

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organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Aeration rate, Bioreactor cultivation, Escherichia coli, Phytase production, Plasmid stability
in
Journal of Pure and Applied Microbiology
volume
8
issue
4
pages
8 pages
publisher
DR M N KHAN
external identifiers
  • scopus:84908201182
ISSN
0973-7510
language
English
LU publication?
yes
id
060444f7-54b0-460b-a2eb-6424505b8e35
date added to LUP
2019-07-12 04:21:31
date last changed
2021-09-15 05:35:46
@article{060444f7-54b0-460b-a2eb-6424505b8e35,
  abstract     = {<p>Phytase production using Escherichia coli BL21 (DE3), a recombinant strain harboring a plasmid encoding thermostable Bacillus phytase, in semi-industrial scale was studied in this work. Among the factors needed to be considered in order to achieve high enzyme yield, aeration rate plays an important role. Suitable aeration is required to supply cells with sufficient amount of air for cell and phytase production. The effect of different aeration rates, (1.0, 2.0 and 3.0 vv<sup>-1</sup>min<sup>-1</sup>), on the kinetics of cell growth and phytase production by the recombinant E. coli BL21 (DE3) in 16-L pilot scale stirred tank bioreactor was investigated. The highest cell concentration of 3.81 gL<sup>-1</sup> concomitant with the maximal total phytase production of 15.63 UmL<sup>-1</sup> were obtained in a bioreactor of aeration rate 3.0vv<sup>-1</sup>min<sup>-1</sup>. At this high aeration rate, a maximal specific growth rate (μ<sub>max</sub>) and the maximal specific phytase production of 0.33 h<sup>-1</sup> and 4102Ug<sup>-1</sup>, respectively, were achieved.</p>},
  author       = {Othman, Nor Zalina and Elsayed, Elsayed A. and Malek, Roslinda Abd and Ramli, Solleh and Masri, Helmy J. and Sarmidi, Mohamed R. and Aziz, Ramlan and Wadaan, Mohamad A. and Hatti-Kaul, Rajni and El Enshasy, Hesham A.},
  issn         = {0973-7510},
  language     = {eng},
  month        = {01},
  number       = {4},
  pages        = {2721--2728},
  publisher    = {DR M N KHAN},
  series       = {Journal of Pure and Applied Microbiology},
  title        = {Aeration rate effect on the growth kinetics, phytase production and plasmid stability of recombinant Escherichia coli BL21 (DE3)},
  volume       = {8},
  year         = {2014},
}