Transcription factor profiling in individual hematopoietic progenitors by digital RT-PCR
(2006) In Proceedings of the National Academy of Sciences 103(47). p.17807-17812- Abstract
- We report here a systematic, quantitative population analysis of transcription factor expression within developmental progenitors, made possible by a microfluidic chip-based "digital RT-PCR" assay that can count template molecules in cDNA samples prepared from single cells. In a survey encompassing five classes of early hematopoietic precursor, we found markedly heterogeneous expression of the transcription factor PU.1 in hematopoietic stem cells and divergent patterns of PU.1 expression within flk2(-) and flk2(+) common myeloid progenitors. The survey also revealed significant differences in the level of the housekeeping transcript GAPDH across the surveyed populations, which demonstrates caveats of normalizing expression data to... (More)
- We report here a systematic, quantitative population analysis of transcription factor expression within developmental progenitors, made possible by a microfluidic chip-based "digital RT-PCR" assay that can count template molecules in cDNA samples prepared from single cells. In a survey encompassing five classes of early hematopoietic precursor, we found markedly heterogeneous expression of the transcription factor PU.1 in hematopoietic stem cells and divergent patterns of PU.1 expression within flk2(-) and flk2(+) common myeloid progenitors. The survey also revealed significant differences in the level of the housekeeping transcript GAPDH across the surveyed populations, which demonstrates caveats of normalizing expression data to endogenous controls and underscores the need to put gene measurement on an absolute, copy-per-cell basis. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/683906
- author
- Warren, Luigi ; Bryder, David LU ; Weissman, Irving L. and Quake, Stephen R.
- organization
- publishing date
- 2006
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- PU.1, microfluidics, gene profiling, hematopoiesis, stem cells
- in
- Proceedings of the National Academy of Sciences
- volume
- 103
- issue
- 47
- pages
- 17807 - 17812
- publisher
- National Academy of Sciences
- external identifiers
-
- wos:000242464900042
- scopus:33845218548
- pmid:17098862
- ISSN
- 1091-6490
- DOI
- 10.1073/pnas.0608512103
- language
- English
- LU publication?
- yes
- additional info
- The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Hematopoietic Stem Cell Laboratory (013022012), Division of Molecular Hematology (DMH) (013017011)
- id
- 06a37421-6cee-421b-b17f-f4366b01270c (old id 683906)
- date added to LUP
- 2016-04-01 12:37:56
- date last changed
- 2022-04-06 00:59:52
@article{06a37421-6cee-421b-b17f-f4366b01270c,
abstract = {{We report here a systematic, quantitative population analysis of transcription factor expression within developmental progenitors, made possible by a microfluidic chip-based "digital RT-PCR" assay that can count template molecules in cDNA samples prepared from single cells. In a survey encompassing five classes of early hematopoietic precursor, we found markedly heterogeneous expression of the transcription factor PU.1 in hematopoietic stem cells and divergent patterns of PU.1 expression within flk2(-) and flk2(+) common myeloid progenitors. The survey also revealed significant differences in the level of the housekeeping transcript GAPDH across the surveyed populations, which demonstrates caveats of normalizing expression data to endogenous controls and underscores the need to put gene measurement on an absolute, copy-per-cell basis.}},
author = {{Warren, Luigi and Bryder, David and Weissman, Irving L. and Quake, Stephen R.}},
issn = {{1091-6490}},
keywords = {{PU.1; microfluidics; gene profiling; hematopoiesis; stem cells}},
language = {{eng}},
number = {{47}},
pages = {{17807--17812}},
publisher = {{National Academy of Sciences}},
series = {{Proceedings of the National Academy of Sciences}},
title = {{Transcription factor profiling in individual hematopoietic progenitors by digital RT-PCR}},
url = {{http://dx.doi.org/10.1073/pnas.0608512103}},
doi = {{10.1073/pnas.0608512103}},
volume = {{103}},
year = {{2006}},
}