Separation of parasites from human blood using deterministic lateral displacement.
Holm, Stefan ; Beech, Jason LU ; Barrett, Michael P and Tegenfeldt, Jonas LU
(2011)
In Lab on a Chip
11(Online February 2011).
p.1326-1332
- Abstract
- We present the use of a simple microfluidic technique to separate living parasites from human blood. Parasitic trypanosomatids cause a range of human and animal diseases. African trypanosomes, responsible for human African trypanosomiasis (sleeping sickness), live free in the blood and other tissue fluids. Diagnosis relies on detection and due to their often low numbers against an overwhelming background of predominantly red blood cells it is crucial to separate the parasites from the blood. By modifying the method of deterministic lateral displacement, confining parasites and red blood cells in channels of optimized depth which accentuates morphological differences, we were able to achieve separation thus offering a potential route to... (More)
- We present the use of a simple microfluidic technique to separate living parasites from human blood. Parasitic trypanosomatids cause a range of human and animal diseases. African trypanosomes, responsible for human African trypanosomiasis (sleeping sickness), live free in the blood and other tissue fluids. Diagnosis relies on detection and due to their often low numbers against an overwhelming background of predominantly red blood cells it is crucial to separate the parasites from the blood. By modifying the method of deterministic lateral displacement, confining parasites and red blood cells in channels of optimized depth which accentuates morphological differences, we were able to achieve separation thus offering a potential route to diagnostics. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1831679
- author
- Holm, Stefan
; Beech, Jason
LU
; Barrett, Michael P
and Tegenfeldt, Jonas
LU
- organization
- publishing date
- 2011
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Lab on a Chip
- volume
- 11
- issue
- Online February 2011
- pages
- 1326 - 1332
- publisher
- Royal Society of Chemistry
- external identifiers
-
- wos:000288455100020
- pmid:21331436
- scopus:79952665138
- ISSN
- 1473-0189
- DOI
- 10.1039/c0lc00560f
- language
- English
- LU publication?
- yes
- id
- 06b3ef2f-14f3-4cd1-87ea-0e347f1e9359 (old id 1831679)
- date added to LUP
- 2016-04-01 10:04:10
- date last changed
- 2023-11-09 11:06:09
@article{06b3ef2f-14f3-4cd1-87ea-0e347f1e9359,
abstract = {{We present the use of a simple microfluidic technique to separate living parasites from human blood. Parasitic trypanosomatids cause a range of human and animal diseases. African trypanosomes, responsible for human African trypanosomiasis (sleeping sickness), live free in the blood and other tissue fluids. Diagnosis relies on detection and due to their often low numbers against an overwhelming background of predominantly red blood cells it is crucial to separate the parasites from the blood. By modifying the method of deterministic lateral displacement, confining parasites and red blood cells in channels of optimized depth which accentuates morphological differences, we were able to achieve separation thus offering a potential route to diagnostics.}},
author = {{Holm, Stefan and Beech, Jason and Barrett, Michael P and Tegenfeldt, Jonas}},
issn = {{1473-0189}},
language = {{eng}},
number = {{Online February 2011}},
pages = {{1326--1332}},
publisher = {{Royal Society of Chemistry}},
series = {{Lab on a Chip}},
title = {{Separation of parasites from human blood using deterministic lateral displacement.}},
url = {{http://dx.doi.org/10.1039/c0lc00560f}},
doi = {{10.1039/c0lc00560f}},
volume = {{11}},
year = {{2011}},
}