Microbial carbon use efficiency and priming of soil organic matter mineralization by glucose additions in boreal forest soils with different C:N ratios
(2022) In Soil Biology and Biochemistry 167.- Abstract
During the last decade it has been increasingly acknowledged that carbon (C) contained in root exudates can accelerate decomposition of soil organic matter (SOM), a phenomenon known as rhizosphere priming effect (RPE). However, the controlling factors and the role of different soil microorganisms in RPE are not yet well understood. There are some indications that the response of the soil microbial decomposers to labile C input in the rhizosphere depends on microbial demand of nutrients for growth and maintenance, especially that of C and nitrogen (N). To test this hypothesis, we assessed SOM decomposition induced by 13C-glucose additions during one week in forest soils with different C:N ratios (11.5–22.2). We estimated SOM... (More)
During the last decade it has been increasingly acknowledged that carbon (C) contained in root exudates can accelerate decomposition of soil organic matter (SOM), a phenomenon known as rhizosphere priming effect (RPE). However, the controlling factors and the role of different soil microorganisms in RPE are not yet well understood. There are some indications that the response of the soil microbial decomposers to labile C input in the rhizosphere depends on microbial demand of nutrients for growth and maintenance, especially that of C and nitrogen (N). To test this hypothesis, we assessed SOM decomposition induced by 13C-glucose additions during one week in forest soils with different C:N ratios (11.5–22.2). We estimated SOM respiration, the potential activity (concentration) of a range of extracellular enzymes, and incorporation of 13C and deuterium (D) in microbial phospholipid fatty acids (PLFAs). Glucose additions induced positive priming (a 12–52% increase in SOM respiration) in all soil types, but there was no linear relationship between priming and the soil C:N ratio. Instead, priming of SOM respiration was positively linked to the C:N imbalance, where a higher C:N imbalance implies stronger microbial N limitation. The total oxidative enzyme activity and the ratio between the activities of C and N acquiring enzymes were lower in soil with higher C:N ratios, but these findings could not be quantitatively linked to the observed priming rates. It appears as if glucose addition resulted in priming by stimulating the activity rather than the concentration of oxidative enzymes. Microbial incorporation of D and 13C into in PLFAs demonstrated that glucose additions stimulated both fungal and bacterial growth. The increased growth was mainly supported by glucose assimilation in fungi, while the increase in bacterial growth partly was a result of increased availability of C or N released from SOM. Taken together, the findings suggest that the soil C:N ratio is a poor predictor of priming and that priming is more dependent on the C:N imbalance, which reflects both microbial nutrient demand and nutrient provision.
(Less)
- author
- Karhu, Kristiina ; Alaei, Saeed LU ; Li, Jian LU ; Merilä, Päivi ; Ostonen, Ivika and Bengtson, Per LU
- organization
- publishing date
- 2022-04-01
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- 13C, Carbon use efficiency, D, N mining, PLFA, Priming effect
- in
- Soil Biology and Biochemistry
- volume
- 167
- article number
- 108615
- publisher
- Elsevier
- external identifiers
-
- scopus:85125263233
- ISSN
- 0038-0717
- DOI
- 10.1016/j.soilbio.2022.108615
- language
- English
- LU publication?
- yes
- id
- 06e6968b-900b-45ed-a7ab-f421059535af
- date added to LUP
- 2022-06-08 14:29:27
- date last changed
- 2022-06-08 14:29:27
@article{06e6968b-900b-45ed-a7ab-f421059535af,
abstract = {{<p>During the last decade it has been increasingly acknowledged that carbon (C) contained in root exudates can accelerate decomposition of soil organic matter (SOM), a phenomenon known as rhizosphere priming effect (RPE). However, the controlling factors and the role of different soil microorganisms in RPE are not yet well understood. There are some indications that the response of the soil microbial decomposers to labile C input in the rhizosphere depends on microbial demand of nutrients for growth and maintenance, especially that of C and nitrogen (N). To test this hypothesis, we assessed SOM decomposition induced by <sup>13</sup>C-glucose additions during one week in forest soils with different C:N ratios (11.5–22.2). We estimated SOM respiration, the potential activity (concentration) of a range of extracellular enzymes, and incorporation of <sup>13</sup>C and deuterium (D) in microbial phospholipid fatty acids (PLFAs). Glucose additions induced positive priming (a 12–52% increase in SOM respiration) in all soil types, but there was no linear relationship between priming and the soil C:N ratio. Instead, priming of SOM respiration was positively linked to the C:N imbalance, where a higher C:N imbalance implies stronger microbial N limitation. The total oxidative enzyme activity and the ratio between the activities of C and N acquiring enzymes were lower in soil with higher C:N ratios, but these findings could not be quantitatively linked to the observed priming rates. It appears as if glucose addition resulted in priming by stimulating the activity rather than the concentration of oxidative enzymes. Microbial incorporation of D and <sup>13</sup>C into in PLFAs demonstrated that glucose additions stimulated both fungal and bacterial growth. The increased growth was mainly supported by glucose assimilation in fungi, while the increase in bacterial growth partly was a result of increased availability of C or N released from SOM. Taken together, the findings suggest that the soil C:N ratio is a poor predictor of priming and that priming is more dependent on the C:N imbalance, which reflects both microbial nutrient demand and nutrient provision.</p>}},
author = {{Karhu, Kristiina and Alaei, Saeed and Li, Jian and Merilä, Päivi and Ostonen, Ivika and Bengtson, Per}},
issn = {{0038-0717}},
keywords = {{13C; Carbon use efficiency; D; N mining; PLFA; Priming effect}},
language = {{eng}},
month = {{04}},
publisher = {{Elsevier}},
series = {{Soil Biology and Biochemistry}},
title = {{Microbial carbon use efficiency and priming of soil organic matter mineralization by glucose additions in boreal forest soils with different C:N ratios}},
url = {{http://dx.doi.org/10.1016/j.soilbio.2022.108615}},
doi = {{10.1016/j.soilbio.2022.108615}},
volume = {{167}},
year = {{2022}},
}