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LETM proteins play a role in the accumulation of mitochondrially encoded proteins in Arabidopsis thaliana and AtLETM2 displays parent of origin effects

Zhang, Botao; Carrie, Chris; Ivanova, Aneta; Narsai, Reena; Murcha, Monika W; Duncan, Owen; Wang, Yan; Law, Simon R; Albrecht, Verónica and Pogson, Barry James, et al. (2012) In Journal of Biological Chemistry 287(50). p.73-41757
Abstract

The Arabidopsis thaliana genome contains two genes with homology to the mitochondrial protein LETM1 (leucine zipper-EF-hand-containing transmembrane protein). Inactivation of both genes, Atletm1 and Atletm2, together is lethal. Plants that are hemizygous for AtLETM2 and homozygous for Atletm1 (letm1(-/-) LETM2(+/-)) displayed a mild retarded growth phenotype during early seedling growth. It was shown that accumulation of mitochondrial proteins was reduced in hemizygous (letm1(-/-) LETM2(+/-)) plants. Examination of respiratory chain proteins by Western blotting, blue native PAGE, and enzymatic activity assays revealed that the steady state level of ATP synthase was reduced in abundance, whereas the steady state levels of other... (More)

The Arabidopsis thaliana genome contains two genes with homology to the mitochondrial protein LETM1 (leucine zipper-EF-hand-containing transmembrane protein). Inactivation of both genes, Atletm1 and Atletm2, together is lethal. Plants that are hemizygous for AtLETM2 and homozygous for Atletm1 (letm1(-/-) LETM2(+/-)) displayed a mild retarded growth phenotype during early seedling growth. It was shown that accumulation of mitochondrial proteins was reduced in hemizygous (letm1(-/-) LETM2(+/-)) plants. Examination of respiratory chain proteins by Western blotting, blue native PAGE, and enzymatic activity assays revealed that the steady state level of ATP synthase was reduced in abundance, whereas the steady state levels of other respiratory chain proteins remained unchanged. The absence of a functional maternal AtLETM2 allele in an Atletm1 mutant background resulted in early seed abortion. Reciprocal crosses revealed that maternally, but not paternally, derived AtLETM2 was absolutely required for seed development. This requirement for a functional maternal allele of AtLETM2 was confirmed using direct sequencing of reciprocal crosses of Col-0 and Ler accessions. Furthermore, AtLETM2 promoter β-glucuronidase constructs displayed exclusive maternal expression patterns.

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publication status
published
subject
keywords
Arabidopsis, Arabidopsis Proteins, Electron Transport, Gene Expression Regulation, Plant, Mitochondria, Mitochondrial Proteins
in
Journal of Biological Chemistry
volume
287
issue
50
pages
17 pages
publisher
ASBMB
external identifiers
  • scopus:84871311489
ISSN
1083-351X
DOI
10.1074/jbc.M112.383836
language
English
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no
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06faf07a-1528-4c03-9491-8ea46508891b
date added to LUP
2017-05-09 10:06:51
date last changed
2017-05-30 13:26:39
@article{06faf07a-1528-4c03-9491-8ea46508891b,
  abstract     = {<p>The Arabidopsis thaliana genome contains two genes with homology to the mitochondrial protein LETM1 (leucine zipper-EF-hand-containing transmembrane protein). Inactivation of both genes, Atletm1 and Atletm2, together is lethal. Plants that are hemizygous for AtLETM2 and homozygous for Atletm1 (letm1(-/-) LETM2(+/-)) displayed a mild retarded growth phenotype during early seedling growth. It was shown that accumulation of mitochondrial proteins was reduced in hemizygous (letm1(-/-) LETM2(+/-)) plants. Examination of respiratory chain proteins by Western blotting, blue native PAGE, and enzymatic activity assays revealed that the steady state level of ATP synthase was reduced in abundance, whereas the steady state levels of other respiratory chain proteins remained unchanged. The absence of a functional maternal AtLETM2 allele in an Atletm1 mutant background resulted in early seed abortion. Reciprocal crosses revealed that maternally, but not paternally, derived AtLETM2 was absolutely required for seed development. This requirement for a functional maternal allele of AtLETM2 was confirmed using direct sequencing of reciprocal crosses of Col-0 and Ler accessions. Furthermore, AtLETM2 promoter β-glucuronidase constructs displayed exclusive maternal expression patterns.</p>},
  author       = {Zhang, Botao and Carrie, Chris and Ivanova, Aneta and Narsai, Reena and Murcha, Monika W and Duncan, Owen and Wang, Yan and Law, Simon R and Albrecht, Verónica and Pogson, Barry James and Giraud, Estelle and Van Aken, Olivier and Whelan, James},
  issn         = {1083-351X},
  keyword      = {Arabidopsis,Arabidopsis Proteins,Electron Transport,Gene Expression Regulation, Plant,Mitochondria,Mitochondrial Proteins},
  language     = {eng},
  month        = {12},
  number       = {50},
  pages        = {73--41757},
  publisher    = {ASBMB},
  series       = {Journal of Biological Chemistry},
  title        = {LETM proteins play a role in the accumulation of mitochondrially encoded proteins in Arabidopsis thaliana and AtLETM2 displays parent of origin effects},
  url          = {http://dx.doi.org/10.1074/jbc.M112.383836},
  volume       = {287},
  year         = {2012},
}