CD99 is a novel prognostic stromal marker in non-small cell lung cancer
(2012) In International Journal of Cancer 131(10). p.2264-2273- Abstract
- The complex interaction between cancer cells and the microenvironment plays an essential role in all stages of tumourigenesis. Despite the significance of this interplay, alterations in protein composition underlying tumourstroma interactions are largely unknown. The aim of this study was to identify stromal proteins with clinical relevance in non-small cell lung cancer (NSCLC). A list encompassing 203 stromal candidate genes was compiled based on gene expression array data and available literature. The protein expression of these genes in human NSCLC was screened using the Human Protein Atlas. Twelve proteins were selected that showed a differential stromal staining pattern (BGN, CD99, DCN, EMILIN1, FBN1, PDGFRB, PDLIM5, POSTN, SPARC,... (More)
- The complex interaction between cancer cells and the microenvironment plays an essential role in all stages of tumourigenesis. Despite the significance of this interplay, alterations in protein composition underlying tumourstroma interactions are largely unknown. The aim of this study was to identify stromal proteins with clinical relevance in non-small cell lung cancer (NSCLC). A list encompassing 203 stromal candidate genes was compiled based on gene expression array data and available literature. The protein expression of these genes in human NSCLC was screened using the Human Protein Atlas. Twelve proteins were selected that showed a differential stromal staining pattern (BGN, CD99, DCN, EMILIN1, FBN1, PDGFRB, PDLIM5, POSTN, SPARC, TAGLN, TNC and VCAN). The corresponding antibodies were applied on tissue microarrays, including 190 NSCLC samples, and stromal staining was correlated with clinical parameters. Higher stromal expression of CD99 was associated with better prognosis in the univariate (p = 0.037) and multivariate (p = 0.039) analysis. The association was independent from the proportion of tumour stroma, the fraction of inflammatory cells and clinical and pathological parameters like stage, performance status and tumour histology. The prognostic impact of stromal CD99 protein expression was confirmed in an independent cohort of 240 NSCLC patients (p = 0.008). Furthermore, double-staining confocal fluorescence microscopy showed that CD99 was expressed in stromal lymphocytes as well as in cancer-associated fibroblasts. Based on a comprehensive screening strategy the membrane protein CD99 was identified as a novel stromal factor with clinical relevance. The results support the concept that stromal properties have an important impact on tumour progression. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/3189565
- author
- organization
- publishing date
- 2012
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- CD99, CAF, tumour stroma, gene expression, lung cancer, tissue, microarray
- in
- International Journal of Cancer
- volume
- 131
- issue
- 10
- pages
- 2264 - 2273
- publisher
- John Wiley & Sons Inc.
- external identifiers
-
- wos:000309185300007
- scopus:84867063079
- pmid:22392539
- ISSN
- 0020-7136
- DOI
- 10.1002/ijc.27518
- language
- English
- LU publication?
- yes
- additional info
- The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Pathology, (Lund) (013030000)
- id
- 075685b4-c15a-4bd4-8a53-ece6d03435a3 (old id 3189565)
- date added to LUP
- 2016-04-01 10:05:11
- date last changed
- 2024-01-06 07:14:39
@article{075685b4-c15a-4bd4-8a53-ece6d03435a3, abstract = {{The complex interaction between cancer cells and the microenvironment plays an essential role in all stages of tumourigenesis. Despite the significance of this interplay, alterations in protein composition underlying tumourstroma interactions are largely unknown. The aim of this study was to identify stromal proteins with clinical relevance in non-small cell lung cancer (NSCLC). A list encompassing 203 stromal candidate genes was compiled based on gene expression array data and available literature. The protein expression of these genes in human NSCLC was screened using the Human Protein Atlas. Twelve proteins were selected that showed a differential stromal staining pattern (BGN, CD99, DCN, EMILIN1, FBN1, PDGFRB, PDLIM5, POSTN, SPARC, TAGLN, TNC and VCAN). The corresponding antibodies were applied on tissue microarrays, including 190 NSCLC samples, and stromal staining was correlated with clinical parameters. Higher stromal expression of CD99 was associated with better prognosis in the univariate (p = 0.037) and multivariate (p = 0.039) analysis. The association was independent from the proportion of tumour stroma, the fraction of inflammatory cells and clinical and pathological parameters like stage, performance status and tumour histology. The prognostic impact of stromal CD99 protein expression was confirmed in an independent cohort of 240 NSCLC patients (p = 0.008). Furthermore, double-staining confocal fluorescence microscopy showed that CD99 was expressed in stromal lymphocytes as well as in cancer-associated fibroblasts. Based on a comprehensive screening strategy the membrane protein CD99 was identified as a novel stromal factor with clinical relevance. The results support the concept that stromal properties have an important impact on tumour progression.}}, author = {{Edlund, Karolina and Lindskog, Cecilia and Saito, Akira and Berglund, Anders and Ponten, Fredrik and Goransson-Kultima, Hanna and Isaksson, Anders and Jirström, Karin and Planck, Maria and Johansson, Leif and Lambe, Mats and Holmberg, Lars and Nyberg, Fredrik and Ekman, Simon and Bergqvist, Michael and Landelius, Per and Lamberg, Kristina and Botling, Johan and Ostman, Arne and Micke, Patrick}}, issn = {{0020-7136}}, keywords = {{CD99; CAF; tumour stroma; gene expression; lung cancer; tissue; microarray}}, language = {{eng}}, number = {{10}}, pages = {{2264--2273}}, publisher = {{John Wiley & Sons Inc.}}, series = {{International Journal of Cancer}}, title = {{CD99 is a novel prognostic stromal marker in non-small cell lung cancer}}, url = {{http://dx.doi.org/10.1002/ijc.27518}}, doi = {{10.1002/ijc.27518}}, volume = {{131}}, year = {{2012}}, }