Reduction of primary graft dysfunction using cytokine adsorption during organ preservation and after lung transplantation
(2022) In Nature Communications 13(1).- Abstract
Despite improvements, lung transplantation remains hampered by both a scarcity of donor organs and by mortality following primary graft dysfunction (PGD). Since acute respiratory distress syndrome (ARDS) limits donor lungs utilization, we investigated cytokine adsorption as a means of treating ARDS donor lungs. We induced mild to moderate ARDS using lipopolysaccharide in 16 donor pigs. Lungs were then treated with or without cytokine adsorption during ex vivo lung perfusion (EVLP) and/or post-transplantation using extracorporeal hemoperfusion. The treatment significantly decreased cytokine levels during EVLP and decreased levels of immune cells post-transplantation. Histology demonstrated fewer signs of lung injury across both treatment... (More)
Despite improvements, lung transplantation remains hampered by both a scarcity of donor organs and by mortality following primary graft dysfunction (PGD). Since acute respiratory distress syndrome (ARDS) limits donor lungs utilization, we investigated cytokine adsorption as a means of treating ARDS donor lungs. We induced mild to moderate ARDS using lipopolysaccharide in 16 donor pigs. Lungs were then treated with or without cytokine adsorption during ex vivo lung perfusion (EVLP) and/or post-transplantation using extracorporeal hemoperfusion. The treatment significantly decreased cytokine levels during EVLP and decreased levels of immune cells post-transplantation. Histology demonstrated fewer signs of lung injury across both treatment periods and the incidence of PGD was significantly reduced among treated animals. Overall, cytokine adsorption was able to restore lung function and reduce PGD in lung transplantation. We suggest this treatment will increase the availability of donor lungs and increase the tolerability of donor lungs in the recipient.
(Less)
- author
- organization
-
- StemTherapy: National Initiative on Stem Cells for Regenerative Therapy
- Thoracic Surgery
- WCMM-Wallenberg Centre for Molecular Medicine
- Stem Cell Center
- Anesthesiology and Intensive Care
- Lung Bioengineering and Regeneration (research group)
- Pediatric anesthesia and intensive care (research group)
- Clinical and experimental lung transplantation (research group)
- NanoLund: Centre for Nanoscience
- LTH Profile Area: Engineering Health
- DCD transplantation of lungs (research group)
- NPWT technology (research group)
- publishing date
- 2022-07-26
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Nature Communications
- volume
- 13
- issue
- 1
- article number
- 4173
- publisher
- Nature Publishing Group
- external identifiers
-
- scopus:85135021848
- pmid:35882835
- ISSN
- 2041-1723
- DOI
- 10.1038/s41467-022-31811-5
- language
- English
- LU publication?
- yes
- id
- 07b7fd00-a25c-4ea4-bf65-eea825d60bd0
- date added to LUP
- 2022-07-28 13:34:32
- date last changed
- 2024-10-30 21:41:08
@article{07b7fd00-a25c-4ea4-bf65-eea825d60bd0, abstract = {{<p>Despite improvements, lung transplantation remains hampered by both a scarcity of donor organs and by mortality following primary graft dysfunction (PGD). Since acute respiratory distress syndrome (ARDS) limits donor lungs utilization, we investigated cytokine adsorption as a means of treating ARDS donor lungs. We induced mild to moderate ARDS using lipopolysaccharide in 16 donor pigs. Lungs were then treated with or without cytokine adsorption during ex vivo lung perfusion (EVLP) and/or post-transplantation using extracorporeal hemoperfusion. The treatment significantly decreased cytokine levels during EVLP and decreased levels of immune cells post-transplantation. Histology demonstrated fewer signs of lung injury across both treatment periods and the incidence of PGD was significantly reduced among treated animals. Overall, cytokine adsorption was able to restore lung function and reduce PGD in lung transplantation. We suggest this treatment will increase the availability of donor lungs and increase the tolerability of donor lungs in the recipient.</p>}}, author = {{Ghaidan, Haider and Stenlo, Martin and Niroomand, Anna and Mittendorfer, Margareta and Hirdman, Gabriel and Gvazava, Nika and Edström, Dag and Augusto Silva, Iran and Broberg, Ellen and Hallgren, Oskar and Olm, Franziska and Wagner, Darcy E and Pierre, Leif and Hyllén, Snejana and Lindstedt, Sandra}}, issn = {{2041-1723}}, language = {{eng}}, month = {{07}}, number = {{1}}, publisher = {{Nature Publishing Group}}, series = {{Nature Communications}}, title = {{Reduction of primary graft dysfunction using cytokine adsorption during organ preservation and after lung transplantation}}, url = {{http://dx.doi.org/10.1038/s41467-022-31811-5}}, doi = {{10.1038/s41467-022-31811-5}}, volume = {{13}}, year = {{2022}}, }