Dietary factors influence the recovery rates of Helicobacter pylori in a BALB/cA mouse model
(1998) In Zentralblatt für Bakteriologie: Ternational Journal of Medical Microbiology: Medical Microbiology, Virology, Parasitology, Infectious Diseases 288(2). p.195-205- Abstract
- The aim of this study was to assess the ability of different mouse diets to sustain an H. pylori infection in BALB/cA mice. Four commercially available mouse diets were compared. Experiment 1: Mice were fed the four diets for seven days before infection, infected three times at two-day intervals with 0.1 ml of 10(9) colony-forming units/ml H. pylori cells. H. pylori strains (n = 4) were cultured on GAB-Camp agar for 2 days, harvested and suspended in PBS. All animals were sacrificed at 2 and 4 weeks post inoculation. Experiment 2: Mice infected for 8 weeks were fed RM2, changed to the different diets for 10 days and sacrificed. Stomachs were collected, cultured on GAB-Camp agar to estimate H. pylori growth and stomach biopsies were... (More)
- The aim of this study was to assess the ability of different mouse diets to sustain an H. pylori infection in BALB/cA mice. Four commercially available mouse diets were compared. Experiment 1: Mice were fed the four diets for seven days before infection, infected three times at two-day intervals with 0.1 ml of 10(9) colony-forming units/ml H. pylori cells. H. pylori strains (n = 4) were cultured on GAB-Camp agar for 2 days, harvested and suspended in PBS. All animals were sacrificed at 2 and 4 weeks post inoculation. Experiment 2: Mice infected for 8 weeks were fed RM2, changed to the different diets for 10 days and sacrificed. Stomachs were collected, cultured on GAB-Camp agar to estimate H. pylori growth and stomach biopsies were analyzed by PCR. There were significant differences between diets in their ability to sustain growth of H. pylori. The range was from a few hundred colonies to no growth at all on the GAB-Camp agar. PCR signals showed good correlation with the culture results. All H. pylori-infected mice gave a significantly higher inflammation score compared to non-infected mice. The diet RM2, having the highest number of culturable H. pylori in the mouse stomach, also showed the highest inflammation. These results suggest that the dietary factors affect the amounts of H. pylori in an infection of BALB/cA mice. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1113265
- author
- Wang, Xin LU ; Sjunnesson, Håkan LU ; Sturegård, Erik LU ; Wadström, Torkel LU ; Willen, R and Aleljung, P
- organization
- publishing date
- 1998
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Zentralblatt für Bakteriologie: Ternational Journal of Medical Microbiology: Medical Microbiology, Virology, Parasitology, Infectious Diseases
- volume
- 288
- issue
- 2
- pages
- 195 - 205
- publisher
- Fischer
- external identifiers
-
- pmid:9809401
- scopus:0032191569
- ISSN
- 0934-8840
- language
- English
- LU publication?
- yes
- additional info
- The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Clinical Microbiology, Malmö (013011000), Division of Health Economics and Forensic Medicine (Closed 2012) (013040050), Division of Medical Microbiology (013250400)
- id
- 07d74f9a-94fa-401e-b6f9-fa712072fb22 (old id 1113265)
- date added to LUP
- 2016-04-01 16:16:06
- date last changed
- 2022-01-28 18:28:37
@article{07d74f9a-94fa-401e-b6f9-fa712072fb22, abstract = {{The aim of this study was to assess the ability of different mouse diets to sustain an H. pylori infection in BALB/cA mice. Four commercially available mouse diets were compared. Experiment 1: Mice were fed the four diets for seven days before infection, infected three times at two-day intervals with 0.1 ml of 10(9) colony-forming units/ml H. pylori cells. H. pylori strains (n = 4) were cultured on GAB-Camp agar for 2 days, harvested and suspended in PBS. All animals were sacrificed at 2 and 4 weeks post inoculation. Experiment 2: Mice infected for 8 weeks were fed RM2, changed to the different diets for 10 days and sacrificed. Stomachs were collected, cultured on GAB-Camp agar to estimate H. pylori growth and stomach biopsies were analyzed by PCR. There were significant differences between diets in their ability to sustain growth of H. pylori. The range was from a few hundred colonies to no growth at all on the GAB-Camp agar. PCR signals showed good correlation with the culture results. All H. pylori-infected mice gave a significantly higher inflammation score compared to non-infected mice. The diet RM2, having the highest number of culturable H. pylori in the mouse stomach, also showed the highest inflammation. These results suggest that the dietary factors affect the amounts of H. pylori in an infection of BALB/cA mice.}}, author = {{Wang, Xin and Sjunnesson, Håkan and Sturegård, Erik and Wadström, Torkel and Willen, R and Aleljung, P}}, issn = {{0934-8840}}, language = {{eng}}, number = {{2}}, pages = {{195--205}}, publisher = {{Fischer}}, series = {{Zentralblatt für Bakteriologie: Ternational Journal of Medical Microbiology: Medical Microbiology, Virology, Parasitology, Infectious Diseases}}, title = {{Dietary factors influence the recovery rates of Helicobacter pylori in a BALB/cA mouse model}}, volume = {{288}}, year = {{1998}}, }