Pre-PCR processing strategies
(2004)- Abstract
- A review describes the sample prepn. for biochem. methods, enrichment methods, immunol. methods, and phys. methods. The pretreatment of a complex biol. sample is crucial, and for successful PCR the following requirements have to be fulfilled: complete lack or low concn. of PCR-inhibitory components in the sample and sufficient concn. of target DNA. The aim of the pre-PCR treatment is to convert a complex biol. sample contg. the target microorganisms into PCR-amplifiable samples.
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/530653
- author
- Rådström, Peter LU ; Lövenklev, Maria LU ; Wolffs, Petra LU ; Löfström, Charlotta LU and Knutsson, Rickard LU
- organization
- publishing date
- 2004
- type
- Chapter in Book/Report/Conference proceeding
- publication status
- published
- subject
- host publication
- PCR technology : current innovations
- publisher
- CRC Press
- external identifiers
-
- scopus:4344599509
- ISBN
- 0-8493-1184-5
- language
- English
- LU publication?
- yes
- id
- 081bb468-cd74-4805-b47e-96dd2890826c (old id 530653)
- date added to LUP
- 2016-04-04 10:16:54
- date last changed
- 2022-04-08 05:21:57
@inbook{081bb468-cd74-4805-b47e-96dd2890826c, abstract = {{A review describes the sample prepn. for biochem. methods, enrichment methods, immunol. methods, and phys. methods. The pretreatment of a complex biol. sample is crucial, and for successful PCR the following requirements have to be fulfilled: complete lack or low concn. of PCR-inhibitory components in the sample and sufficient concn. of target DNA. The aim of the pre-PCR treatment is to convert a complex biol. sample contg. the target microorganisms into PCR-amplifiable samples.}}, author = {{Rådström, Peter and Lövenklev, Maria and Wolffs, Petra and Löfström, Charlotta and Knutsson, Rickard}}, booktitle = {{PCR technology : current innovations}}, isbn = {{0-8493-1184-5}}, language = {{eng}}, publisher = {{CRC Press}}, title = {{Pre-PCR processing strategies}}, year = {{2004}}, }