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Comparisons of Immunoassay and Mass Spectrometry Measurements of Serum Estradiol Levels and Their Influence on Clinical Association Studies in Men.

Ohlsson, Claes; Nilsson, Maria E LU ; Tivesten, Asa; Ryberg, Henrik; Mellström, Dan; Karlsson, Magnus LU ; Ljunggren, Osten; Labrie, Fernand; Orwoll, Eric S and Lee, David M, et al. (2013) In Journal of Clinical Endocrinology and Metabolism 98(6). p.1097-1102
Abstract
Context:Immunoassay-based techniques, routinely used to measure serum estradiol (E2), are known to have reduced specificity, especially at lower concentrations, when compared with the gold standard technique of mass spectrometry (MS). Different measurement techniques may be responsible for the conflicting results of associations between serum E2 and clinical phenotypes in men.Objective:Our objective was to compare immunoassay and MS measurements of E2 levels in men and evaluate associations with clinical phenotypes.Design and Setting:Middle-aged and older male subjects participating in the population-based Osteoporotic Fractures in Men (MrOS) Sweden study (n = 2599), MrOS US (n = 688), and the European Male Aging Study (n = 2908) were... (More)
Context:Immunoassay-based techniques, routinely used to measure serum estradiol (E2), are known to have reduced specificity, especially at lower concentrations, when compared with the gold standard technique of mass spectrometry (MS). Different measurement techniques may be responsible for the conflicting results of associations between serum E2 and clinical phenotypes in men.Objective:Our objective was to compare immunoassay and MS measurements of E2 levels in men and evaluate associations with clinical phenotypes.Design and Setting:Middle-aged and older male subjects participating in the population-based Osteoporotic Fractures in Men (MrOS) Sweden study (n = 2599), MrOS US (n = 688), and the European Male Aging Study (n = 2908) were included.Main Outcome Measures:Immunoassay and MS measurements of serum E2 were compared and related to bone mineral density (BMD; measured by dual energy x-ray absorptiometry) and ankle-brachial index.Results:Within each cohort, serum E2 levels obtained by immunoassay and MS correlated moderately (Spearman rank correlation coefficient rS 0.53-0.76). Serum C-reactive protein (CRP) levels associated significantly (albeit to a low extent, rS = 0.29) with immunoassay E2 but not with MS E2 levels. Similar associations of immunoassay E2 and MS E2 were seen with lumbar spine and total hip BMD, independent of serum CRP. However, immunoassay E2, but not MS E2, associated inversely with ankle-brachial index, and this correlation was lost after adjustment for CRP.Conclusions:Our findings suggest interference in the immunoassay E2 analyses, possibly by CRP or a CRP-associated factor. Although associations with BMD remain unaffected, this might imply for a reevaluation of previous association studies between immunoassay E2 levels and inflammation-related outcomes. (Less)
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published
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Journal of Clinical Endocrinology and Metabolism
volume
98
issue
6
pages
1097 - 1102
publisher
The Endocrine Society
external identifiers
  • wos:000319736500010
  • pmid:23633197
  • scopus:84878476231
ISSN
1945-7197
DOI
10.1210/jc.2012-3861
language
English
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089d86e9-c2c2-4446-bd91-73a3db796298 (old id 3805073)
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http://www.ncbi.nlm.nih.gov/pubmed/23633197?dopt=Abstract
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2013-06-03 16:18:50
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2019-01-13 03:22:14
@article{089d86e9-c2c2-4446-bd91-73a3db796298,
  abstract     = {Context:Immunoassay-based techniques, routinely used to measure serum estradiol (E2), are known to have reduced specificity, especially at lower concentrations, when compared with the gold standard technique of mass spectrometry (MS). Different measurement techniques may be responsible for the conflicting results of associations between serum E2 and clinical phenotypes in men.Objective:Our objective was to compare immunoassay and MS measurements of E2 levels in men and evaluate associations with clinical phenotypes.Design and Setting:Middle-aged and older male subjects participating in the population-based Osteoporotic Fractures in Men (MrOS) Sweden study (n = 2599), MrOS US (n = 688), and the European Male Aging Study (n = 2908) were included.Main Outcome Measures:Immunoassay and MS measurements of serum E2 were compared and related to bone mineral density (BMD; measured by dual energy x-ray absorptiometry) and ankle-brachial index.Results:Within each cohort, serum E2 levels obtained by immunoassay and MS correlated moderately (Spearman rank correlation coefficient rS 0.53-0.76). Serum C-reactive protein (CRP) levels associated significantly (albeit to a low extent, rS = 0.29) with immunoassay E2 but not with MS E2 levels. Similar associations of immunoassay E2 and MS E2 were seen with lumbar spine and total hip BMD, independent of serum CRP. However, immunoassay E2, but not MS E2, associated inversely with ankle-brachial index, and this correlation was lost after adjustment for CRP.Conclusions:Our findings suggest interference in the immunoassay E2 analyses, possibly by CRP or a CRP-associated factor. Although associations with BMD remain unaffected, this might imply for a reevaluation of previous association studies between immunoassay E2 levels and inflammation-related outcomes.},
  author       = {Ohlsson, Claes and Nilsson, Maria E and Tivesten, Asa and Ryberg, Henrik and Mellström, Dan and Karlsson, Magnus and Ljunggren, Osten and Labrie, Fernand and Orwoll, Eric S and Lee, David M and Pye, Stephen R and O'Neill, Terence W and Finn, Joseph D and Adams, Judith E and Ward, Kate A and Boonen, Steven and Bartfai, Gyorgy and Casanueva, Felipe F and Forti, Gianni and Giwercman, Aleksander and Han, Thang S and Huhtaniemi, Ilpo T and Kula, Krzysztof and Lean, Michael E J and Pendleton, Neil and Punab, Margus and Vanderschueren, Dirk and Wu, Frederick C W and Vandenput, Liesbeth},
  issn         = {1945-7197},
  language     = {eng},
  number       = {6},
  pages        = {1097--1102},
  publisher    = {The Endocrine Society},
  series       = {Journal of Clinical Endocrinology and Metabolism},
  title        = {Comparisons of Immunoassay and Mass Spectrometry Measurements of Serum Estradiol Levels and Their Influence on Clinical Association Studies in Men.},
  url          = {http://dx.doi.org/10.1210/jc.2012-3861},
  volume       = {98},
  year         = {2013},
}