Co-administration with A1M does not influence apoptotic response of 177Lu-octreotate in GOT1 neuroendocrine tumors
Rassol, Nishte ; Andersson, Charlotte ; Pettersson, Daniella ; Al-Awar, Amin ; Shubbar, Emman ; Kovács, Anikó ; Åkerström, Bo LU ; Gram, Magnus LU
; Helou, Khalil
and Forssell-Aronsson, Eva
(2023)
In Scientific Reports
13(1).
- Abstract
Recombinant α1-microglobulin (A1M) is a proposed radioprotector during 177Lu-octreotate therapy of neuroendocrine tumors (NETs). To ensure a maintained therapeutic effect, we previously demonstrated that A1M does not affect the 177Lu-octreotate induced decrease in GOT1 tumor volume. However, the underlying biological events of these findings are still unknown. The aim of this work was to examine the regulation of apoptosis-related genes in GOT1 tumors short-time after i.v. administration of 177Lu-octreotate with and without A1M or A1M alone. Human GOT1 tumor-bearing mice received 30 MBq 177Lu-octreotate or 5 mg/kg A1M or co-treatment with both. Animals were sacrificed after 1 or 7... (More)
Recombinant α1-microglobulin (A1M) is a proposed radioprotector during 177Lu-octreotate therapy of neuroendocrine tumors (NETs). To ensure a maintained therapeutic effect, we previously demonstrated that A1M does not affect the 177Lu-octreotate induced decrease in GOT1 tumor volume. However, the underlying biological events of these findings are still unknown. The aim of this work was to examine the regulation of apoptosis-related genes in GOT1 tumors short-time after i.v. administration of 177Lu-octreotate with and without A1M or A1M alone. Human GOT1 tumor-bearing mice received 30 MBq 177Lu-octreotate or 5 mg/kg A1M or co-treatment with both. Animals were sacrificed after 1 or 7 days. Gene expression analysis of apoptosis-related genes in GOT1 tissue was performed with RT-PCR. In general, similar expression patterns of pro- and anti-apoptotic genes were found after 177Lu-octreotate exposure with or without co-administration of A1M. The highest regulated genes in both irradiated groups compared to untreated controls were FAS and TNFSFRS10B. Administration of A1M alone only resulted in significantly regulated genes after 7 days. Co-administration of A1M did not negatively affect the transcriptional apoptotic response of 177Lu-octreotate in GOT1 tumors.
(Less)
- author
- Rassol, Nishte
; Andersson, Charlotte
; Pettersson, Daniella
; Al-Awar, Amin
; Shubbar, Emman
; Kovács, Anikó
; Åkerström, Bo
LU
; Gram, Magnus
LU
; Helou, Khalil
and Forssell-Aronsson, Eva
- organization
- publishing date
- 2023
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Scientific Reports
- volume
- 13
- issue
- 1
- article number
- 6417
- publisher
- Nature Publishing Group
- external identifiers
-
- pmid:37076494
- scopus:85152979450
- ISSN
- 2045-2322
- DOI
- 10.1038/s41598-023-32091-9
- language
- English
- LU publication?
- yes
- id
- 08e03ae6-d4ab-4395-a872-57a2d5dfbfbe
- date added to LUP
- 2023-07-12 14:20:57
- date last changed
- 2024-04-19 23:20:07
@article{08e03ae6-d4ab-4395-a872-57a2d5dfbfbe,
abstract = {{<p>Recombinant α<sub>1</sub>-microglobulin (A1M) is a proposed radioprotector during <sup>177</sup>Lu-octreotate therapy of neuroendocrine tumors (NETs). To ensure a maintained therapeutic effect, we previously demonstrated that A1M does not affect the <sup>177</sup>Lu-octreotate induced decrease in GOT1 tumor volume. However, the underlying biological events of these findings are still unknown. The aim of this work was to examine the regulation of apoptosis-related genes in GOT1 tumors short-time after i.v. administration of <sup>177</sup>Lu-octreotate with and without A1M or A1M alone. Human GOT1 tumor-bearing mice received 30 MBq <sup>177</sup>Lu-octreotate or 5 mg/kg A1M or co-treatment with both. Animals were sacrificed after 1 or 7 days. Gene expression analysis of apoptosis-related genes in GOT1 tissue was performed with RT-PCR. In general, similar expression patterns of pro- and anti-apoptotic genes were found after <sup>177</sup>Lu-octreotate exposure with or without co-administration of A1M. The highest regulated genes in both irradiated groups compared to untreated controls were FAS and TNFSFRS10B. Administration of A1M alone only resulted in significantly regulated genes after 7 days. Co-administration of A1M did not negatively affect the transcriptional apoptotic response of <sup>177</sup>Lu-octreotate in GOT1 tumors.</p>}},
author = {{Rassol, Nishte and Andersson, Charlotte and Pettersson, Daniella and Al-Awar, Amin and Shubbar, Emman and Kovács, Anikó and Åkerström, Bo and Gram, Magnus and Helou, Khalil and Forssell-Aronsson, Eva}},
issn = {{2045-2322}},
language = {{eng}},
number = {{1}},
publisher = {{Nature Publishing Group}},
series = {{Scientific Reports}},
title = {{Co-administration with A1M does not influence apoptotic response of <sup>177</sup>Lu-octreotate in GOT1 neuroendocrine tumors}},
url = {{http://dx.doi.org/10.1038/s41598-023-32091-9}},
doi = {{10.1038/s41598-023-32091-9}},
volume = {{13}},
year = {{2023}},
}