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Epigenetic alterations in blood mirror age-associated DNA methylation and gene expression changes in human liver

Bysani, Madhusudhan LU ; Perfilyev, Alexander LU ; de Mello, Vanessa; Rönn, Tina LU ; Nilsson, Emma LU ; Pihlajamäki, Jussi and Ling, Charlotte LU (2017) In Epigenomics 9(2). p.105-122
Abstract

Aim: To study the impact of aging on DNA methylation and mRNA expression in human liver. Experimental procedures: We analysed genome-wide DNA methylation and gene expression in human liver samples using Illumina 450K and HumanHT12 expression BeadChip arrays. Results: DNA methylation analysis of ∼455,000 CpG sites in human liver revealed that age was significantly associated with altered DNA methylation of 20,396 CpG sites. Comparison of liver methylation data with published methylation data in other tissues showed that vast majority of the age-associated significant CpG sites overlapped between liver and blood, whereas a smaller overlap was found between liver and pancreatic islets or adipose tissue, respectively. We identified 151... (More)

Aim: To study the impact of aging on DNA methylation and mRNA expression in human liver. Experimental procedures: We analysed genome-wide DNA methylation and gene expression in human liver samples using Illumina 450K and HumanHT12 expression BeadChip arrays. Results: DNA methylation analysis of ∼455,000 CpG sites in human liver revealed that age was significantly associated with altered DNA methylation of 20,396 CpG sites. Comparison of liver methylation data with published methylation data in other tissues showed that vast majority of the age-associated significant CpG sites overlapped between liver and blood, whereas a smaller overlap was found between liver and pancreatic islets or adipose tissue, respectively. We identified 151 genes whose liver expression also correlated with age. Conclusions: We identified age-associated DNA methylation and expression changes in human liver that are partly reflected by epigenetic alterations in blood.

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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
aging, blood, diabetes, DNA methylation, ELOVL2, epigenetics, FHL2, gene expression, KLF14, liver
in
Epigenomics
volume
9
issue
2
pages
18 pages
publisher
Future Medicine Ltd.
external identifiers
  • scopus:85010288585
  • wos:000393452600004
ISSN
1750-1911
DOI
10.2217/epi-2016-0087
language
English
LU publication?
yes
id
0960d760-7c59-4cc2-b06b-273add99dbe9
date added to LUP
2017-02-03 13:27:45
date last changed
2018-01-07 11:48:06
@article{0960d760-7c59-4cc2-b06b-273add99dbe9,
  abstract     = {<p>Aim: To study the impact of aging on DNA methylation and mRNA expression in human liver. Experimental procedures: We analysed genome-wide DNA methylation and gene expression in human liver samples using Illumina 450K and HumanHT12 expression BeadChip arrays. Results: DNA methylation analysis of ∼455,000 CpG sites in human liver revealed that age was significantly associated with altered DNA methylation of 20,396 CpG sites. Comparison of liver methylation data with published methylation data in other tissues showed that vast majority of the age-associated significant CpG sites overlapped between liver and blood, whereas a smaller overlap was found between liver and pancreatic islets or adipose tissue, respectively. We identified 151 genes whose liver expression also correlated with age. Conclusions: We identified age-associated DNA methylation and expression changes in human liver that are partly reflected by epigenetic alterations in blood.</p>},
  author       = {Bysani, Madhusudhan and Perfilyev, Alexander and de Mello, Vanessa and Rönn, Tina and Nilsson, Emma and Pihlajamäki, Jussi and Ling, Charlotte},
  issn         = {1750-1911},
  keyword      = {aging,blood,diabetes,DNA methylation,ELOVL2,epigenetics,FHL2,gene expression,KLF14,liver},
  language     = {eng},
  month        = {02},
  number       = {2},
  pages        = {105--122},
  publisher    = {Future Medicine Ltd.},
  series       = {Epigenomics},
  title        = {Epigenetic alterations in blood mirror age-associated DNA methylation and gene expression changes in human liver},
  url          = {http://dx.doi.org/10.2217/epi-2016-0087},
  volume       = {9},
  year         = {2017},
}