Engineering CGTase to improve synthesis of alkyl glycosides
(2021) In Glycobiology 31(5). p.603-612- Abstract
Alkyl glycoside surfactants with elongated carbohydrate chains are useful in different applications due to their improved biocompatibility. Cyclodextrin glucanotransferases can catalyse the elongation process through the coupling reaction. However, due to the presence of a hydrophobic tail, the interaction between an alkyl glycoside acceptor and the active site residues is weaker than the interaction with maltooligosaccharides at the corresponding site. Here we report the mutations of F197, G263 and E266 near the acceptor subsites in the CGTase CspCGT13 from Carboxydocella sp. The results showed that substitutions of both F197 and G263 were important for the binding of acceptor substrate dodecyl maltoside during coupling... (More)
Alkyl glycoside surfactants with elongated carbohydrate chains are useful in different applications due to their improved biocompatibility. Cyclodextrin glucanotransferases can catalyse the elongation process through the coupling reaction. However, due to the presence of a hydrophobic tail, the interaction between an alkyl glycoside acceptor and the active site residues is weaker than the interaction with maltooligosaccharides at the corresponding site. Here we report the mutations of F197, G263 and E266 near the acceptor subsites in the CGTase CspCGT13 from Carboxydocella sp. The results showed that substitutions of both F197 and G263 were important for the binding of acceptor substrate dodecyl maltoside during coupling reaction. The double mutant F197Y/G263A showed enhanced coupling activity and displayed a 2-fold increase of the primary coupling product using γ-cyclodextrin as donor when compared to wildtype CspCGT13. Disproportionation activity was also reduced, which was also the case for another double mutant (F197Y/E266A) that however not showed the corresponding increase in coupling. A triple mutant F197Y/G263A/E266A maintained the increase in primary coupling product (1.8-fold increase) using dodecyl maltoside as acceptor, but disproportionation was approximately at the same level as in the double mutants. In addition, hydrolysis of starch was slightly increased by the F197Y and G263A substitutions, indicating that interactions at both positions influenced the selectivity between glycosyl and alkyl moieties.
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- author
- Ara, Kazi Zubaida Gulshan ; Linares-Pastén, Javier A LU ; Jönsson, Jonas ; Viloria-Cols, Maria LU ; Ulvenlund, Stefan LU ; Adlercreutz, Patrick LU and Karlsson, Eva Nordberg LU
- organization
- publishing date
- 2021-05-01
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Acceptor subsites, Cyclodextrin glycosyltransferase, Coupling reaction, dodecyl-β-maltoside, γ-Cyclodextrin
- in
- Glycobiology
- volume
- 31
- issue
- 5
- article number
- cwaa109
- pages
- 10 pages
- publisher
- Oxford University Press
- external identifiers
-
- scopus:85107902497
- pmid:33270133
- ISSN
- 1460-2423
- DOI
- 10.1093/glycob/cwaa109
- language
- English
- LU publication?
- yes
- id
- 099f666d-4b26-476f-a351-a746f99dc689
- date added to LUP
- 2020-12-06 21:57:58
- date last changed
- 2024-09-19 10:52:30
@article{099f666d-4b26-476f-a351-a746f99dc689, abstract = {{<p>Alkyl glycoside surfactants with elongated carbohydrate chains are useful in different applications due to their improved biocompatibility. Cyclodextrin glucanotransferases can catalyse the elongation process through the coupling reaction. However, due to the presence of a hydrophobic tail, the interaction between an alkyl glycoside acceptor and the active site residues is weaker than the interaction with maltooligosaccharides at the corresponding site. Here we report the mutations of F197, G263 and E266 near the acceptor subsites in the CGTase CspCGT13 from <i>Carboxydocella</i> sp. The results showed that substitutions of both F197 and G263 were important for the binding of acceptor substrate dodecyl maltoside during coupling reaction. The double mutant F197Y/G263A showed enhanced coupling activity and displayed a 2-fold increase of the primary coupling product using γ-cyclodextrin as donor when compared to wildtype CspCGT13. Disproportionation activity was also reduced, which was also the case for another double mutant (F197Y/E266A) that however not showed the corresponding increase in coupling. A triple mutant F197Y/G263A/E266A maintained the increase in primary coupling product (1.8-fold increase) using dodecyl maltoside as acceptor, but disproportionation was approximately at the same level as in the double mutants. In addition, hydrolysis of starch was slightly increased by the F197Y and G263A substitutions, indicating that interactions at both positions influenced the selectivity between glycosyl and alkyl moieties.</p>}}, author = {{Ara, Kazi Zubaida Gulshan and Linares-Pastén, Javier A and Jönsson, Jonas and Viloria-Cols, Maria and Ulvenlund, Stefan and Adlercreutz, Patrick and Karlsson, Eva Nordberg}}, issn = {{1460-2423}}, keywords = {{Acceptor subsites; Cyclodextrin glycosyltransferase; Coupling reaction; dodecyl-β-maltoside; γ-Cyclodextrin}}, language = {{eng}}, month = {{05}}, number = {{5}}, pages = {{603--612}}, publisher = {{Oxford University Press}}, series = {{Glycobiology}}, title = {{Engineering CGTase to improve synthesis of alkyl glycosides}}, url = {{http://dx.doi.org/10.1093/glycob/cwaa109}}, doi = {{10.1093/glycob/cwaa109}}, volume = {{31}}, year = {{2021}}, }