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Changes in mACh, NMDA and GABA(A) receptor binding after lateral fluid-percussion injury : in vitro autoradiography of rat brain frozen sections

Sihver, S; Marklund, N LU ; Hillered, L; Långström, B; Watanabe, Y and Bergström, M (2001) In Journal of Neurochemistry 78(3). p.23-417
Abstract

Adult rats were subjected to a moderate lateral fluid percussion injury (FPI), followed by survival periods of 2 and 12 h. Regional NMDA subtype glutamate, muscarinic acetylcholine and GABA(A) receptor binding in various brain regions was analysed by quantitative in vitro autoradiography and short-lived positron emission tomography tracers [11C]cyano-dizocilpine, 4-N-[11C]methylpiperidylbenzilate (4-N-[11C]MPB), and [11C]flumazenil, respectively. The binding potential (BP, Bmax/KD) was calculated. The data with [11C]cyano-dizocilpine showed a significant decrease in BP bilaterally for the frontoparietal cortex and hippocampus at both time points, in comparison with that of the sham-operated controls. At 12 h the decrease was... (More)

Adult rats were subjected to a moderate lateral fluid percussion injury (FPI), followed by survival periods of 2 and 12 h. Regional NMDA subtype glutamate, muscarinic acetylcholine and GABA(A) receptor binding in various brain regions was analysed by quantitative in vitro autoradiography and short-lived positron emission tomography tracers [11C]cyano-dizocilpine, 4-N-[11C]methylpiperidylbenzilate (4-N-[11C]MPB), and [11C]flumazenil, respectively. The binding potential (BP, Bmax/KD) was calculated. The data with [11C]cyano-dizocilpine showed a significant decrease in BP bilaterally for the frontoparietal cortex and hippocampus at both time points, in comparison with that of the sham-operated controls. At 12 h the decrease was significantly more prominent for the ipsilateral cortex and hippocampus than for the contralateral side. The BP of 4-N-[11C]MPB was significantly decreased after 2 h for the trauma-side hippocampus, and after 12 h it had decreased for the trauma-site cortex and the bilateral hippocampus. The [11C]flumazenil exhibited a significant decrease in BP for the trauma-site cortex and the underlying hippocampus by 2 h after the traumatic brain injury. After 12 h a significantly decreased BP was observed only for the trauma-site cortex. The finding of a decreased BP demonstrates the involvement of these receptor systems in the development of cellular dysfunction, which is widespread and not limited to the site of lateral FPI.

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published
keywords
Animals, Autoradiography, Binding Sites, Brain, Brain Injuries, Cerebral Cortex, Dizocilpine Maleate, Flumazenil, Frozen Sections, Hippocampus, Humans, Kinetics, Male, Nitriles, Piperidines, Rats, Rats, Sprague-Dawley, Receptors, GABA-A, Receptors, Muscarinic, Receptors, N-Methyl-D-Aspartate, Tomography, Emission-Computed, Journal Article
in
Journal of Neurochemistry
volume
78
issue
3
pages
23 - 417
publisher
Wiley-Blackwell
external identifiers
  • scopus:0034904325
ISSN
0022-3042
DOI
10.1046/j.1471-4159.2001.00428.x
language
English
LU publication?
no
id
09f73eee-d163-47c1-b8dc-456b51988d15
date added to LUP
2018-03-03 17:28:01
date last changed
2018-05-29 11:55:23
@article{09f73eee-d163-47c1-b8dc-456b51988d15,
  abstract     = {<p>Adult rats were subjected to a moderate lateral fluid percussion injury (FPI), followed by survival periods of 2 and 12 h. Regional NMDA subtype glutamate, muscarinic acetylcholine and GABA(A) receptor binding in various brain regions was analysed by quantitative in vitro autoradiography and short-lived positron emission tomography tracers [11C]cyano-dizocilpine, 4-N-[11C]methylpiperidylbenzilate (4-N-[11C]MPB), and [11C]flumazenil, respectively. The binding potential (BP, Bmax/KD) was calculated. The data with [11C]cyano-dizocilpine showed a significant decrease in BP bilaterally for the frontoparietal cortex and hippocampus at both time points, in comparison with that of the sham-operated controls. At 12 h the decrease was significantly more prominent for the ipsilateral cortex and hippocampus than for the contralateral side. The BP of 4-N-[11C]MPB was significantly decreased after 2 h for the trauma-side hippocampus, and after 12 h it had decreased for the trauma-site cortex and the bilateral hippocampus. The [11C]flumazenil exhibited a significant decrease in BP for the trauma-site cortex and the underlying hippocampus by 2 h after the traumatic brain injury. After 12 h a significantly decreased BP was observed only for the trauma-site cortex. The finding of a decreased BP demonstrates the involvement of these receptor systems in the development of cellular dysfunction, which is widespread and not limited to the site of lateral FPI.</p>},
  author       = {Sihver, S and Marklund, N and Hillered, L and Långström, B and Watanabe, Y and Bergström, M},
  issn         = {0022-3042},
  keyword      = {Animals,Autoradiography,Binding Sites,Brain,Brain Injuries,Cerebral Cortex,Dizocilpine Maleate,Flumazenil,Frozen Sections,Hippocampus,Humans,Kinetics,Male,Nitriles,Piperidines,Rats,Rats, Sprague-Dawley,Receptors, GABA-A,Receptors, Muscarinic,Receptors, N-Methyl-D-Aspartate,Tomography, Emission-Computed,Journal Article},
  language     = {eng},
  number       = {3},
  pages        = {23--417},
  publisher    = {Wiley-Blackwell},
  series       = {Journal of Neurochemistry},
  title        = {Changes in mACh, NMDA and GABA(A) receptor binding after lateral fluid-percussion injury : in vitro autoradiography of rat brain frozen sections},
  url          = {http://dx.doi.org/10.1046/j.1471-4159.2001.00428.x},
  volume       = {78},
  year         = {2001},
}