Changes in mACh, NMDA and GABA(A) receptor binding after lateral fluid-percussion injury : in vitro autoradiography of rat brain frozen sections
Sihver, S ; Marklund, N LU
; Hillered, L
; Långström, B
; Watanabe, Y
and Bergström, M
(2001)
In Journal of Neurochemistry
78(3).
p.23-417
- Abstract
Adult rats were subjected to a moderate lateral fluid percussion injury (FPI), followed by survival periods of 2 and 12 h. Regional NMDA subtype glutamate, muscarinic acetylcholine and GABA(A) receptor binding in various brain regions was analysed by quantitative in vitro autoradiography and short-lived positron emission tomography tracers [11C]cyano-dizocilpine, 4-N-[11C]methylpiperidylbenzilate (4-N-[11C]MPB), and [11C]flumazenil, respectively. The binding potential (BP, Bmax/KD) was calculated. The data with [11C]cyano-dizocilpine showed a significant decrease in BP bilaterally for the frontoparietal cortex and hippocampus at both time points, in comparison with that of the sham-operated controls. At 12 h the decrease was... (More)
Adult rats were subjected to a moderate lateral fluid percussion injury (FPI), followed by survival periods of 2 and 12 h. Regional NMDA subtype glutamate, muscarinic acetylcholine and GABA(A) receptor binding in various brain regions was analysed by quantitative in vitro autoradiography and short-lived positron emission tomography tracers [11C]cyano-dizocilpine, 4-N-[11C]methylpiperidylbenzilate (4-N-[11C]MPB), and [11C]flumazenil, respectively. The binding potential (BP, Bmax/KD) was calculated. The data with [11C]cyano-dizocilpine showed a significant decrease in BP bilaterally for the frontoparietal cortex and hippocampus at both time points, in comparison with that of the sham-operated controls. At 12 h the decrease was significantly more prominent for the ipsilateral cortex and hippocampus than for the contralateral side. The BP of 4-N-[11C]MPB was significantly decreased after 2 h for the trauma-side hippocampus, and after 12 h it had decreased for the trauma-site cortex and the bilateral hippocampus. The [11C]flumazenil exhibited a significant decrease in BP for the trauma-site cortex and the underlying hippocampus by 2 h after the traumatic brain injury. After 12 h a significantly decreased BP was observed only for the trauma-site cortex. The finding of a decreased BP demonstrates the involvement of these receptor systems in the development of cellular dysfunction, which is widespread and not limited to the site of lateral FPI.
(Less)
- author
- Sihver, S
; Marklund, N
LU
; Hillered, L
; Långström, B
; Watanabe, Y
and Bergström, M
- publishing date
- 2001-08
- type
- Contribution to journal
- publication status
- published
- keywords
- Animals, Autoradiography, Binding Sites, Brain, Brain Injuries, Cerebral Cortex, Dizocilpine Maleate, Flumazenil, Frozen Sections, Hippocampus, Humans, Kinetics, Male, Nitriles, Piperidines, Rats, Rats, Sprague-Dawley, Receptors, GABA-A, Receptors, Muscarinic, Receptors, N-Methyl-D-Aspartate, Tomography, Emission-Computed, Journal Article
- in
- Journal of Neurochemistry
- volume
- 78
- issue
- 3
- pages
- 23 - 417
- publisher
- Wiley-Blackwell
- external identifiers
-
- scopus:0034904325
- pmid:11483644
- ISSN
- 0022-3042
- DOI
- 10.1046/j.1471-4159.2001.00428.x
- language
- English
- LU publication?
- no
- id
- 09f73eee-d163-47c1-b8dc-456b51988d15
- date added to LUP
- 2018-03-03 17:28:01
- date last changed
- 2024-04-01 00:15:47
@article{09f73eee-d163-47c1-b8dc-456b51988d15,
abstract = {{<p>Adult rats were subjected to a moderate lateral fluid percussion injury (FPI), followed by survival periods of 2 and 12 h. Regional NMDA subtype glutamate, muscarinic acetylcholine and GABA(A) receptor binding in various brain regions was analysed by quantitative in vitro autoradiography and short-lived positron emission tomography tracers [11C]cyano-dizocilpine, 4-N-[11C]methylpiperidylbenzilate (4-N-[11C]MPB), and [11C]flumazenil, respectively. The binding potential (BP, Bmax/KD) was calculated. The data with [11C]cyano-dizocilpine showed a significant decrease in BP bilaterally for the frontoparietal cortex and hippocampus at both time points, in comparison with that of the sham-operated controls. At 12 h the decrease was significantly more prominent for the ipsilateral cortex and hippocampus than for the contralateral side. The BP of 4-N-[11C]MPB was significantly decreased after 2 h for the trauma-side hippocampus, and after 12 h it had decreased for the trauma-site cortex and the bilateral hippocampus. The [11C]flumazenil exhibited a significant decrease in BP for the trauma-site cortex and the underlying hippocampus by 2 h after the traumatic brain injury. After 12 h a significantly decreased BP was observed only for the trauma-site cortex. The finding of a decreased BP demonstrates the involvement of these receptor systems in the development of cellular dysfunction, which is widespread and not limited to the site of lateral FPI.</p>}},
author = {{Sihver, S and Marklund, N and Hillered, L and Långström, B and Watanabe, Y and Bergström, M}},
issn = {{0022-3042}},
keywords = {{Animals; Autoradiography; Binding Sites; Brain; Brain Injuries; Cerebral Cortex; Dizocilpine Maleate; Flumazenil; Frozen Sections; Hippocampus; Humans; Kinetics; Male; Nitriles; Piperidines; Rats; Rats, Sprague-Dawley; Receptors, GABA-A; Receptors, Muscarinic; Receptors, N-Methyl-D-Aspartate; Tomography, Emission-Computed; Journal Article}},
language = {{eng}},
number = {{3}},
pages = {{23--417}},
publisher = {{Wiley-Blackwell}},
series = {{Journal of Neurochemistry}},
title = {{Changes in mACh, NMDA and GABA(A) receptor binding after lateral fluid-percussion injury : in vitro autoradiography of rat brain frozen sections}},
url = {{http://dx.doi.org/10.1046/j.1471-4159.2001.00428.x}},
doi = {{10.1046/j.1471-4159.2001.00428.x}},
volume = {{78}},
year = {{2001}},
}