Identification of prostate specific antigen (PSA) isoforms in complex biological samples utilizing complementary platforms
Végvári, Ákos LU ; Rezeli, Melinda LU
; Welinder, Charlotte
LU
; Malm, Johan
LU
; Lilja, Hans
LU
; Marko-Varga, György
LU
and Laurell, Thomas
LU
(2010)
In Journal of Proteomics
73(6).
p.1137-1147
- Abstract
- Measurements of the prostate-specific antigen (PSA) levels in blood are widely used as diagnostic, predictive and prognostic marker of prostate disease. The selective detection of molecular forms of PSA can contribute clinically to meaningful enhancements of the conventional PSA-test. As it is plausible that an in-depth search for structural variants of PSA gene products may increase our ability to discriminate distinct patho-biological basis and stages of prostate diseases, we have developed a multi-step protocol comprising gel-based methods followed by mass spectrometric identification.
Our current aim was to provide a comprehensive identification of PSA variants occurring in seminal fluid. We provide a proof-of-principle for... (More) - Measurements of the prostate-specific antigen (PSA) levels in blood are widely used as diagnostic, predictive and prognostic marker of prostate disease. The selective detection of molecular forms of PSA can contribute clinically to meaningful enhancements of the conventional PSA-test. As it is plausible that an in-depth search for structural variants of PSA gene products may increase our ability to discriminate distinct patho-biological basis and stages of prostate diseases, we have developed a multi-step protocol comprising gel-based methods followed by mass spectrometric identification.
Our current aim was to provide a comprehensive identification of PSA variants occurring in seminal fluid. We provide a proof-of-principle for this multiple step analytical approach to identify multiple PSA variants from complex biological samples that revealed distinct molecular characteristics. In addition, sequence-annotated protein bands in SDS–PAGE gels were compared to those detected by Western blots, and by monitoring the enzymatic activity in zymogram gels, using gelatin as a substrate. The high accuracy annotations were obtained by fast turnaround MALDI-Orbitrap analysis from excised and digested gel bands. Multiple PSA forms were identified utilizing a combination of MASCOT and SEQUEST search engines. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1670529
- author
- Végvári, Ákos
LU
; Rezeli, Melinda
LU
; Welinder, Charlotte
LU
; Malm, Johan
LU
; Lilja, Hans
LU
; Marko-Varga, György
LU
and Laurell, Thomas
LU
- organization
- publishing date
- 2010
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Seminal plasma, Mass spectrometry, Prostate-specific antigen isoforms, MALDI LTQ Orbitrap XL
- in
- Journal of Proteomics
- volume
- 73
- issue
- 6
- pages
- 1137 - 1147
- publisher
- Elsevier
- external identifiers
-
- wos:000277763800011
- scopus:77950518665
- pmid:20102753
- ISSN
- 1874-3919
- DOI
- 10.1016/j.jprot.2010.01.008
- language
- English
- LU publication?
- yes
- id
- 0a442c76-4d8b-46cc-8c3f-0dddac561f7a (old id 1670529)
- date added to LUP
- 2016-04-01 11:08:01
- date last changed
- 2023-08-31 19:32:09
@article{0a442c76-4d8b-46cc-8c3f-0dddac561f7a,
abstract = {{Measurements of the prostate-specific antigen (PSA) levels in blood are widely used as diagnostic, predictive and prognostic marker of prostate disease. The selective detection of molecular forms of PSA can contribute clinically to meaningful enhancements of the conventional PSA-test. As it is plausible that an in-depth search for structural variants of PSA gene products may increase our ability to discriminate distinct patho-biological basis and stages of prostate diseases, we have developed a multi-step protocol comprising gel-based methods followed by mass spectrometric identification.<br/><br>
Our current aim was to provide a comprehensive identification of PSA variants occurring in seminal fluid. We provide a proof-of-principle for this multiple step analytical approach to identify multiple PSA variants from complex biological samples that revealed distinct molecular characteristics. In addition, sequence-annotated protein bands in SDS–PAGE gels were compared to those detected by Western blots, and by monitoring the enzymatic activity in zymogram gels, using gelatin as a substrate. The high accuracy annotations were obtained by fast turnaround MALDI-Orbitrap analysis from excised and digested gel bands. Multiple PSA forms were identified utilizing a combination of MASCOT and SEQUEST search engines.}},
author = {{Végvári, Ákos and Rezeli, Melinda and Welinder, Charlotte and Malm, Johan and Lilja, Hans and Marko-Varga, György and Laurell, Thomas}},
issn = {{1874-3919}},
keywords = {{Seminal plasma; Mass spectrometry; Prostate-specific antigen isoforms; MALDI LTQ Orbitrap XL}},
language = {{eng}},
number = {{6}},
pages = {{1137--1147}},
publisher = {{Elsevier}},
series = {{Journal of Proteomics}},
title = {{Identification of prostate specific antigen (PSA) isoforms in complex biological samples utilizing complementary platforms}},
url = {{https://lup.lub.lu.se/search/files/2401683/1670530.pdf}},
doi = {{10.1016/j.jprot.2010.01.008}},
volume = {{73}},
year = {{2010}},
}