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Protocol for identifying cellular reprogramming minimal networks using combinatorial transcription factor screening

Altman, Abigail R. LU orcid ; Pértiga-Cabral, Diogo LU ; Pereira, Carlos Filipe LU orcid and Kurochkin, Ilia LU (2026) In STAR Protocols 7(2).
Abstract

Direct reprogramming offers a powerful approach to generate therapeutic cell types, but progress is limited by an incomplete understanding of transcription factor (TF) cooperativity. Here, we present a protocol for performing combinatorial TF screening to resolve reprogramming factor networks that drive cell identity. We describe steps for arrayed lentiviral production, transduction, and reprogramming of human fibroblasts into distinct immune cells. We detail procedures for cell purification, library preparation, sequencing, and analysis to resolve TF combinations and dynamics. For complete details on the use and execution of this protocol, please refer to Kurochkin et al.

Please use this url to cite or link to this publication:
author
; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
STAR Protocols
volume
7
issue
2
article number
104527
publisher
Cell Press
external identifiers
  • scopus:105037401607
  • pmid:42054212
ISSN
2666-1667
DOI
10.1016/j.xpro.2026.104527
language
English
LU publication?
yes
id
0a58428e-247b-462e-99ca-80e0a203cc63
date added to LUP
2026-05-27 10:24:27
date last changed
2026-05-28 03:00:07
@article{0a58428e-247b-462e-99ca-80e0a203cc63,
  abstract     = {{<p>Direct reprogramming offers a powerful approach to generate therapeutic cell types, but progress is limited by an incomplete understanding of transcription factor (TF) cooperativity. Here, we present a protocol for performing combinatorial TF screening to resolve reprogramming factor networks that drive cell identity. We describe steps for arrayed lentiviral production, transduction, and reprogramming of human fibroblasts into distinct immune cells. We detail procedures for cell purification, library preparation, sequencing, and analysis to resolve TF combinations and dynamics. For complete details on the use and execution of this protocol, please refer to Kurochkin et al.</p>}},
  author       = {{Altman, Abigail R. and Pértiga-Cabral, Diogo and Pereira, Carlos Filipe and Kurochkin, Ilia}},
  issn         = {{2666-1667}},
  language     = {{eng}},
  number       = {{2}},
  publisher    = {{Cell Press}},
  series       = {{STAR Protocols}},
  title        = {{Protocol for identifying cellular reprogramming minimal networks using combinatorial transcription factor screening}},
  url          = {{http://dx.doi.org/10.1016/j.xpro.2026.104527}},
  doi          = {{10.1016/j.xpro.2026.104527}},
  volume       = {{7}},
  year         = {{2026}},
}