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Studies on the Proteome of Human Hair - Identification of Histones and Deamidated Keratins

Adav, Sunil S.; Subbaiaih, Roopa S.; Kerk, Swat Kim; Lee, Amelia Yilin; Lai, Hui Ying; Ng, Kee Woei; Sze, Siu Kwan and Schmidtchen, Artur LU (2018) In Scientific Reports 8(1).
Abstract

Human hair is laminar-fibrous tissue and an evolutionarily old keratinization product of follicle trichocytes. Studies on the hair proteome can give new insights into hair function and lead to the development of novel biomarkers for hair in health and disease. Human hair proteins were extracted by detergent and detergent-free techniques. We adopted a shotgun proteomics approach, which demonstrated a large extractability and variety of hair proteins after detergent extraction. We found an enrichment of keratin, keratin-associated proteins (KAPs), and intermediate filament proteins, which were part of protein networks associated with response to stress, innate immunity, epidermis development, and the hair cycle. Our analysis also revealed... (More)

Human hair is laminar-fibrous tissue and an evolutionarily old keratinization product of follicle trichocytes. Studies on the hair proteome can give new insights into hair function and lead to the development of novel biomarkers for hair in health and disease. Human hair proteins were extracted by detergent and detergent-free techniques. We adopted a shotgun proteomics approach, which demonstrated a large extractability and variety of hair proteins after detergent extraction. We found an enrichment of keratin, keratin-associated proteins (KAPs), and intermediate filament proteins, which were part of protein networks associated with response to stress, innate immunity, epidermis development, and the hair cycle. Our analysis also revealed a significant deamidation of keratin type I and II, and KAPs. The hair shafts were found to contain several types of histones, which are well known to exert antimicrobial activity. Analysis of the hair proteome, particularly its composition, protein abundances, deamidated hair proteins, and modification sites, may offer a novel approach to explore potential biomarkers of hair health quality, hair diseases, and aging.

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published
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in
Scientific Reports
volume
8
issue
1
publisher
Nature Publishing Group
external identifiers
  • scopus:85041021179
ISSN
2045-2322
DOI
language
English
LU publication?
yes
id
0b1e5557-2a55-4e50-bf36-2e389ea8abd2
date added to LUP
2018-02-05 13:18:34
date last changed
2018-05-29 11:55:21
@article{0b1e5557-2a55-4e50-bf36-2e389ea8abd2,
  abstract     = {<p>Human hair is laminar-fibrous tissue and an evolutionarily old keratinization product of follicle trichocytes. Studies on the hair proteome can give new insights into hair function and lead to the development of novel biomarkers for hair in health and disease. Human hair proteins were extracted by detergent and detergent-free techniques. We adopted a shotgun proteomics approach, which demonstrated a large extractability and variety of hair proteins after detergent extraction. We found an enrichment of keratin, keratin-associated proteins (KAPs), and intermediate filament proteins, which were part of protein networks associated with response to stress, innate immunity, epidermis development, and the hair cycle. Our analysis also revealed a significant deamidation of keratin type I and II, and KAPs. The hair shafts were found to contain several types of histones, which are well known to exert antimicrobial activity. Analysis of the hair proteome, particularly its composition, protein abundances, deamidated hair proteins, and modification sites, may offer a novel approach to explore potential biomarkers of hair health quality, hair diseases, and aging.</p>},
  articleno    = {1599},
  author       = {Adav, Sunil S. and Subbaiaih, Roopa S. and Kerk, Swat Kim and Lee, Amelia Yilin and Lai, Hui Ying and Ng, Kee Woei and Sze, Siu Kwan and Schmidtchen, Artur},
  issn         = {2045-2322},
  language     = {eng},
  month        = {12},
  number       = {1},
  publisher    = {Nature Publishing Group},
  series       = {Scientific Reports},
  title        = {Studies on the Proteome of Human Hair - Identification of Histones and Deamidated Keratins},
  url          = {http://dx.doi.org/},
  volume       = {8},
  year         = {2018},
}