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Silver nanoparticles alter cell viability ex vivo and in vitro and induce proinflammatory effects in human lung fibroblasts

Löfdahl, Anna LU ; Jern, Andreas LU ; Flyman, Samuel ; Kåredal, Monica LU orcid ; Karlsson, Hanna L. and Larsson-Callerfelt, Anna Karin LU orcid (2020) In Nanomaterials 10(9).
Abstract

Silver nanoparticles (AgNPs) are commonly used in commercial and medical applications. However, AgNPs may induce toxicity, extracellular matrix (ECM) changes and inflammatory responses. Fibroblasts are key players in remodeling processes and major producers of the ECM. The aims of this study were to explore the effect of AgNPs on cell viability, both ex vivo in murine precision cut lung slices (PCLS) and in vitro in human lung fibroblasts (HFL-1), and immunomodulatory responses in fibroblasts. PCLS and HFL-1 were exposed to AgNPs with different sizes, 10 nm and 75 nm, at concentrations 2 µg/mL and 10 µg/mL. Changes in synthesis of ECM proteins, growth factors and cytokines were analyzed in HFL-1. Ag10 and Ag75 affected cell viability,... (More)

Silver nanoparticles (AgNPs) are commonly used in commercial and medical applications. However, AgNPs may induce toxicity, extracellular matrix (ECM) changes and inflammatory responses. Fibroblasts are key players in remodeling processes and major producers of the ECM. The aims of this study were to explore the effect of AgNPs on cell viability, both ex vivo in murine precision cut lung slices (PCLS) and in vitro in human lung fibroblasts (HFL-1), and immunomodulatory responses in fibroblasts. PCLS and HFL-1 were exposed to AgNPs with different sizes, 10 nm and 75 nm, at concentrations 2 µg/mL and 10 µg/mL. Changes in synthesis of ECM proteins, growth factors and cytokines were analyzed in HFL-1. Ag10 and Ag75 affected cell viability, with significantly reduced metabolic activities at 10 µg/mL in both PCLS and HFL-1 after 48 h. AgNPs significantly increased procollagen I synthesis and release of IL-8, prostaglandin E2, RANTES and eotaxin, whereas reduced IL-6 release was observed in HFL-1 after 72 h. Our data indicate toxic effects of AgNP exposure on cell viability ex vivo and in vitro with altered procollagen and proinflammatory cytokine secretion in fibroblasts over time. Hence, careful characterizations of AgNPs are of importance, and future studies should include timepoints beyond 24 h.

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author
; ; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Cytokines, Extracellular matrix, Growth factors, Human lung fibroblasts, Precision-cut lung slices, Procollagen, Silver nanoparticles, Toxicity
in
Nanomaterials
volume
10
issue
9
article number
1868
pages
12 pages
publisher
MDPI AG
external identifiers
  • scopus:85091518379
  • pmid:32961914
ISSN
2079-4991
DOI
10.3390/nano10091868
language
English
LU publication?
yes
id
0b96c4a4-1f18-43f6-8f51-7b770a6d99c3
date added to LUP
2020-10-27 13:26:28
date last changed
2024-05-15 19:38:29
@article{0b96c4a4-1f18-43f6-8f51-7b770a6d99c3,
  abstract     = {{<p>Silver nanoparticles (AgNPs) are commonly used in commercial and medical applications. However, AgNPs may induce toxicity, extracellular matrix (ECM) changes and inflammatory responses. Fibroblasts are key players in remodeling processes and major producers of the ECM. The aims of this study were to explore the effect of AgNPs on cell viability, both ex vivo in murine precision cut lung slices (PCLS) and in vitro in human lung fibroblasts (HFL-1), and immunomodulatory responses in fibroblasts. PCLS and HFL-1 were exposed to AgNPs with different sizes, 10 nm and 75 nm, at concentrations 2 µg/mL and 10 µg/mL. Changes in synthesis of ECM proteins, growth factors and cytokines were analyzed in HFL-1. Ag10 and Ag75 affected cell viability, with significantly reduced metabolic activities at 10 µg/mL in both PCLS and HFL-1 after 48 h. AgNPs significantly increased procollagen I synthesis and release of IL-8, prostaglandin E<sub>2</sub>, RANTES and eotaxin, whereas reduced IL-6 release was observed in HFL-1 after 72 h. Our data indicate toxic effects of AgNP exposure on cell viability ex vivo and in vitro with altered procollagen and proinflammatory cytokine secretion in fibroblasts over time. Hence, careful characterizations of AgNPs are of importance, and future studies should include timepoints beyond 24 h.</p>}},
  author       = {{Löfdahl, Anna and Jern, Andreas and Flyman, Samuel and Kåredal, Monica and Karlsson, Hanna L. and Larsson-Callerfelt, Anna Karin}},
  issn         = {{2079-4991}},
  keywords     = {{Cytokines; Extracellular matrix; Growth factors; Human lung fibroblasts; Precision-cut lung slices; Procollagen; Silver nanoparticles; Toxicity}},
  language     = {{eng}},
  number       = {{9}},
  publisher    = {{MDPI AG}},
  series       = {{Nanomaterials}},
  title        = {{Silver nanoparticles alter cell viability ex vivo and in vitro and induce proinflammatory effects in human lung fibroblasts}},
  url          = {{http://dx.doi.org/10.3390/nano10091868}},
  doi          = {{10.3390/nano10091868}},
  volume       = {{10}},
  year         = {{2020}},
}