24 h severe fluid restriction increases a biomarker of renal injury in healthy males
Juett, Loris A. ; Funnell, Mark P. ; Carroll, Harriet A. LU
; James, Lewis J.
and Mears, Stephen A.
(2025)
In European Journal of Applied Physiology
- Abstract
Purpose: Exercise-induced hypohydration exacerbates biomarkers of renal injury, but studies isolating the effects of hypohydration without exercise have produced mixed findings. This study investigated the effects of 24-h severe fluid restriction on biomarkers of renal injury and glucose tolerance. Methods: Fifteen males (age: 27 ± 5 y; BMI: 24.1 ± 3.8 kg/m2) completed two randomised trials, involving consuming either 40 mL/kg body mass water to maintain euhydration (EU) or severe fluid restriction via limiting water consumption to 100 mL (HYP). A standardised dry food diet was consumed in both trials (~ 300 g water). At baseline and 24 h post-baseline, nude body mass, and blood and urine samples (additional urine sample at... (More)
Purpose: Exercise-induced hypohydration exacerbates biomarkers of renal injury, but studies isolating the effects of hypohydration without exercise have produced mixed findings. This study investigated the effects of 24-h severe fluid restriction on biomarkers of renal injury and glucose tolerance. Methods: Fifteen males (age: 27 ± 5 y; BMI: 24.1 ± 3.8 kg/m2) completed two randomised trials, involving consuming either 40 mL/kg body mass water to maintain euhydration (EU) or severe fluid restriction via limiting water consumption to 100 mL (HYP). A standardised dry food diet was consumed in both trials (~ 300 g water). At baseline and 24 h post-baseline, nude body mass, and blood and urine samples (additional urine sample at 12 h) were collected. An oral glucose tolerance test was conducted after 24-h post-baseline measurements (n = 12). Results: At 24 h, body mass loss (HYP: − 1.52 ± 0.34%, EU: − 0.24 ± 0.40%), plasma volume loss, serum, and urine osmolality were greater in HYP than EU (P ≤ 0.004). Osmolality-corrected urinary kidney injury molecule-1 (uKIM-1) concentrations were greater in HYP at 12 (HYP: 1.097 ± 0.587 ng/mOsm, EU: 0.570 ± 0.408 ng/mOsm; P < 0.001) and 24-h (HYP: 1.932 ± 1.173 ng/mOsm, EU: 1.599 ± 1.012 ng/mOsm; P = 0.01). There was no trial-by-time interactions for osmolality-corrected urinary neutrophil gelatinase-associated lipocalin concentrations (P = 0.781) or plasma glucose (P = 0.550) and insulin (P = 0.193) concentrations. Conclusion: Hypohydration produced by 24-h fluid restriction increased proximal tubular injury but did not affect glucose tolerance.
(Less)
- author
- Juett, Loris A.
; Funnell, Mark P.
; Carroll, Harriet A.
LU
; James, Lewis J.
and Mears, Stephen A.
- organization
- publishing date
- 2025
- type
- Contribution to journal
- publication status
- epub
- subject
- keywords
- Glucose, Hydration, Hypohydration, Kidney, Water
- in
- European Journal of Applied Physiology
- publisher
- Springer
- external identifiers
-
- pmid:40053075
- scopus:86000349867
- ISSN
- 1439-6319
- DOI
- 10.1007/s00421-025-05749-7
- language
- English
- LU publication?
- yes
- id
- 0c8b3881-d340-4fe7-b296-82b86e280656
- date added to LUP
- 2025-06-26 11:22:59
- date last changed
- 2025-07-10 11:55:21
@article{0c8b3881-d340-4fe7-b296-82b86e280656,
abstract = {{<p>Purpose: Exercise-induced hypohydration exacerbates biomarkers of renal injury, but studies isolating the effects of hypohydration without exercise have produced mixed findings. This study investigated the effects of 24-h severe fluid restriction on biomarkers of renal injury and glucose tolerance. Methods: Fifteen males (age: 27 ± 5 y; BMI: 24.1 ± 3.8 kg/m<sup>2</sup>) completed two randomised trials, involving consuming either 40 mL/kg body mass water to maintain euhydration (EU) or severe fluid restriction via limiting water consumption to 100 mL (HYP). A standardised dry food diet was consumed in both trials (~ 300 g water). At baseline and 24 h post-baseline, nude body mass, and blood and urine samples (additional urine sample at 12 h) were collected. An oral glucose tolerance test was conducted after 24-h post-baseline measurements (n = 12). Results: At 24 h, body mass loss (HYP: − 1.52 ± 0.34%, EU: − 0.24 ± 0.40%), plasma volume loss, serum, and urine osmolality were greater in HYP than EU (P ≤ 0.004). Osmolality-corrected urinary kidney injury molecule-1 (uKIM-1) concentrations were greater in HYP at 12 (HYP: 1.097 ± 0.587 ng/mOsm, EU: 0.570 ± 0.408 ng/mOsm; P < 0.001) and 24-h (HYP: 1.932 ± 1.173 ng/mOsm, EU: 1.599 ± 1.012 ng/mOsm; P = 0.01). There was no trial-by-time interactions for osmolality-corrected urinary neutrophil gelatinase-associated lipocalin concentrations (P = 0.781) or plasma glucose (P = 0.550) and insulin (P = 0.193) concentrations. Conclusion: Hypohydration produced by 24-h fluid restriction increased proximal tubular injury but did not affect glucose tolerance.</p>}},
author = {{Juett, Loris A. and Funnell, Mark P. and Carroll, Harriet A. and James, Lewis J. and Mears, Stephen A.}},
issn = {{1439-6319}},
keywords = {{Glucose; Hydration; Hypohydration; Kidney; Water}},
language = {{eng}},
publisher = {{Springer}},
series = {{European Journal of Applied Physiology}},
title = {{24 h severe fluid restriction increases a biomarker of renal injury in healthy males}},
url = {{http://dx.doi.org/10.1007/s00421-025-05749-7}},
doi = {{10.1007/s00421-025-05749-7}},
year = {{2025}},
}