BPI-ANCA is expressed in the airways of cystic fibrosis patients and correlates to platelet numbers and Pseudomonas aeruginosa colonization
(2020) In Respiratory Medicine 170.- Abstract
Background: Autoantibodies to bactericidal/permeability-increasing protein (BPI), BPI-ANCA, are often present in serum of patients with cystic fibrosis (CF), and correlate to airway colonization with Pseudomonas aeruginosa. The aim of the study was to investigate if BPI-ANCA IgA is also present in the airways of CF patients, and if its presence correlates with neutrophil counts, platelets, and P. aeruginosa DNA in sputum. Methods: BPI-ANCA IgA was quantified in serum and sputum samples from adult CF patients (n = 45) by ELISA. Sputum neutrophil counts, platelets, and platelet-neutrophil complexes were assessed by flow cytometry, and P. aeruginosa DNA was analysed with RT-PCR. Results: Serum BPI-ANCA IgA was present in 44% of the study... (More)
Background: Autoantibodies to bactericidal/permeability-increasing protein (BPI), BPI-ANCA, are often present in serum of patients with cystic fibrosis (CF), and correlate to airway colonization with Pseudomonas aeruginosa. The aim of the study was to investigate if BPI-ANCA IgA is also present in the airways of CF patients, and if its presence correlates with neutrophil counts, platelets, and P. aeruginosa DNA in sputum. Methods: BPI-ANCA IgA was quantified in serum and sputum samples from adult CF patients (n = 45) by ELISA. Sputum neutrophil counts, platelets, and platelet-neutrophil complexes were assessed by flow cytometry, and P. aeruginosa DNA was analysed with RT-PCR. Results: Serum BPI-ANCA IgA was present in 44% of the study participants, and this group also had significantly enhanced BPI-ANCA levels in sputum compared to serum negative patients. Sputum levels of BPI-ANCA IgA correlated with P. aeruginosa DNA (r = 0.63, p = 0.0003) and platelet counts in sputum (r = 0.60, p = 0.0002). Conclusions: BPI-ANCA is expressed in the airways of CF patients and correlates with P. aeruginosa load and platelet counts, suggesting a link to airway inflammation and mucosal immunity.
(Less)
- author
- Hovold, Gisela LU ; Lindberg, Ulrika LU ; Ljungberg, Johanna K. LU ; Shannon, Oonagh LU and Påhlman, Lisa I. LU
- organization
- publishing date
- 2020
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- BPI-ANCA, Cystic fibrosis, Lung function, Platelets, Pseudomonas aeruginosa, Sputum
- in
- Respiratory Medicine
- volume
- 170
- article number
- 105994
- publisher
- Elsevier
- external identifiers
-
- pmid:32843162
- scopus:85086113443
- ISSN
- 0954-6111
- DOI
- 10.1016/j.rmed.2020.105994
- language
- English
- LU publication?
- yes
- id
- 0e08b40b-8f3e-411f-88f1-81730dcd4bc8
- date added to LUP
- 2020-07-01 12:51:29
- date last changed
- 2024-08-21 23:55:03
@article{0e08b40b-8f3e-411f-88f1-81730dcd4bc8, abstract = {{<p>Background: Autoantibodies to bactericidal/permeability-increasing protein (BPI), BPI-ANCA, are often present in serum of patients with cystic fibrosis (CF), and correlate to airway colonization with Pseudomonas aeruginosa. The aim of the study was to investigate if BPI-ANCA IgA is also present in the airways of CF patients, and if its presence correlates with neutrophil counts, platelets, and P. aeruginosa DNA in sputum. Methods: BPI-ANCA IgA was quantified in serum and sputum samples from adult CF patients (n = 45) by ELISA. Sputum neutrophil counts, platelets, and platelet-neutrophil complexes were assessed by flow cytometry, and P. aeruginosa DNA was analysed with RT-PCR. Results: Serum BPI-ANCA IgA was present in 44% of the study participants, and this group also had significantly enhanced BPI-ANCA levels in sputum compared to serum negative patients. Sputum levels of BPI-ANCA IgA correlated with P. aeruginosa DNA (r = 0.63, p = 0.0003) and platelet counts in sputum (r = 0.60, p = 0.0002). Conclusions: BPI-ANCA is expressed in the airways of CF patients and correlates with P. aeruginosa load and platelet counts, suggesting a link to airway inflammation and mucosal immunity.</p>}}, author = {{Hovold, Gisela and Lindberg, Ulrika and Ljungberg, Johanna K. and Shannon, Oonagh and Påhlman, Lisa I.}}, issn = {{0954-6111}}, keywords = {{BPI-ANCA; Cystic fibrosis; Lung function; Platelets; Pseudomonas aeruginosa; Sputum}}, language = {{eng}}, publisher = {{Elsevier}}, series = {{Respiratory Medicine}}, title = {{BPI-ANCA is expressed in the airways of cystic fibrosis patients and correlates to platelet numbers and Pseudomonas aeruginosa colonization}}, url = {{http://dx.doi.org/10.1016/j.rmed.2020.105994}}, doi = {{10.1016/j.rmed.2020.105994}}, volume = {{170}}, year = {{2020}}, }