The pentatricopeptide repeat protein EMP603 is required for the splicing of mitochondrial Nad1 intron 2 and seed development in maize
(2021) In Journal of Experimental Botany 72(20). p.6933-6948- Abstract
Intron splicing is an essential event in post-transcriptional RNA processing in plant mitochondria, which requires the participation of diverse nuclear-encoded splicing factors. However, it is presently unclear how these proteins cooperatively take part in the splicing of specific introns. In this study, we characterized a nuclear-encoded mitochondrial P-type pentatricopeptide repeat (PPR) protein named EMP603. This protein is essential for splicing of intron 2 in the Nad1 gene and interacts with the mitochondria-localized DEAD-box RNA helicase PMH2-5140, the RAD52-like proteins ODB1-0814 and ODB1-5061, and the CRM domain-containing protein Zm-mCSF1. Further study revealed that the N-terminal region of EMP603 interacts with the DEAD-box... (More)
Intron splicing is an essential event in post-transcriptional RNA processing in plant mitochondria, which requires the participation of diverse nuclear-encoded splicing factors. However, it is presently unclear how these proteins cooperatively take part in the splicing of specific introns. In this study, we characterized a nuclear-encoded mitochondrial P-type pentatricopeptide repeat (PPR) protein named EMP603. This protein is essential for splicing of intron 2 in the Nad1 gene and interacts with the mitochondria-localized DEAD-box RNA helicase PMH2-5140, the RAD52-like proteins ODB1-0814 and ODB1-5061, and the CRM domain-containing protein Zm-mCSF1. Further study revealed that the N-terminal region of EMP603 interacts with the DEAD-box of PMH2-5140, the CRM domain of Zm-mCSF1, and OBD1-5061, but not with OBD1-0814, whereas the PPR domain of EMP603 can interact with ODB1-0814, ODB1-5061, and PMH2-5140, but not with Zm-mCSF1. Defects in EMP603 severely disrupt the assembly and activity of mitochondrial complex I, leading to impaired mitochondrial function, and delayed seed development. The interactions revealed between EMP603 and PMH2-5140, ODB1-0814, ODB1-5061, and Zm-mCSF1 indicate a possible involvement of a dynamic 'spliceosome-like' complex in intron splicing, and may accelerate the elucidation of the intron splicing mechanism in plant mitochondria.
(Less)
- author
- Fan, Kaijian ; Ren, Zhenjing ; Zhang, Xiaofeng ; Liu, Yan ; Fu, Junjie ; Qi, Chunlai ; Tatar, Wurinile ; Rasmusson, Allan G. LU ; Wang, Guoying and Liu, Yunjun
- organization
- publishing date
- 2021-10-26
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Emp603, intron splicing, mitochondrion, PPR protein, seed development, Zea mays
- in
- Journal of Experimental Botany
- volume
- 72
- issue
- 20
- pages
- 16 pages
- publisher
- Oxford University Press
- external identifiers
-
- pmid:34279607
- scopus:85117181552
- ISSN
- 0022-0957
- DOI
- 10.1093/jxb/erab339
- language
- English
- LU publication?
- yes
- id
- 0e344b7e-9334-4856-8d16-50f3d98d8329
- date added to LUP
- 2022-02-08 12:48:37
- date last changed
- 2024-04-18 06:24:51
@article{0e344b7e-9334-4856-8d16-50f3d98d8329,
abstract = {{<p>Intron splicing is an essential event in post-transcriptional RNA processing in plant mitochondria, which requires the participation of diverse nuclear-encoded splicing factors. However, it is presently unclear how these proteins cooperatively take part in the splicing of specific introns. In this study, we characterized a nuclear-encoded mitochondrial P-type pentatricopeptide repeat (PPR) protein named EMP603. This protein is essential for splicing of intron 2 in the Nad1 gene and interacts with the mitochondria-localized DEAD-box RNA helicase PMH2-5140, the RAD52-like proteins ODB1-0814 and ODB1-5061, and the CRM domain-containing protein Zm-mCSF1. Further study revealed that the N-terminal region of EMP603 interacts with the DEAD-box of PMH2-5140, the CRM domain of Zm-mCSF1, and OBD1-5061, but not with OBD1-0814, whereas the PPR domain of EMP603 can interact with ODB1-0814, ODB1-5061, and PMH2-5140, but not with Zm-mCSF1. Defects in EMP603 severely disrupt the assembly and activity of mitochondrial complex I, leading to impaired mitochondrial function, and delayed seed development. The interactions revealed between EMP603 and PMH2-5140, ODB1-0814, ODB1-5061, and Zm-mCSF1 indicate a possible involvement of a dynamic 'spliceosome-like' complex in intron splicing, and may accelerate the elucidation of the intron splicing mechanism in plant mitochondria. </p>}},
author = {{Fan, Kaijian and Ren, Zhenjing and Zhang, Xiaofeng and Liu, Yan and Fu, Junjie and Qi, Chunlai and Tatar, Wurinile and Rasmusson, Allan G. and Wang, Guoying and Liu, Yunjun}},
issn = {{0022-0957}},
keywords = {{Emp603; intron splicing; mitochondrion; PPR protein; seed development; Zea mays}},
language = {{eng}},
month = {{10}},
number = {{20}},
pages = {{6933--6948}},
publisher = {{Oxford University Press}},
series = {{Journal of Experimental Botany}},
title = {{The pentatricopeptide repeat protein EMP603 is required for the splicing of mitochondrial Nad1 intron 2 and seed development in maize}},
url = {{http://dx.doi.org/10.1093/jxb/erab339}},
doi = {{10.1093/jxb/erab339}},
volume = {{72}},
year = {{2021}},
}