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The PEF family proteins sorcin and grancalcin interact in vivo and in vitro

Hansen, Christian LU ; Tarabykina, Svetlana ; La Cour, Jonas M. ; Lollike, Karsten and Berchtold, Martin W. (2003) In FEBS Letters 545(2-3). p.151-154
Abstract

The penta-EF hand (PEF) family of calcium binding proteins includes grancalcin, peflin, sorcin, calpain large and small subunits as well as ALG-2. Systematic testing of the heterodimerization abilities of the PEF proteins using the yeast two-hybrid and glutathione S-transferase pull-down assays revealed the new finding that grancalcin interacts strongly with sorcin. In addition, sorcin and grancalcin can be co-immunoprecipitated from lysates of human umbilical vein endothelial cells. Our results indicate that heterodimerization, in addition to differential interactions with target proteins, might be a way to regulate and fine tune processes mediated by calcium binding proteins of the penta-EF hand type.

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author
; ; ; and
publishing date
type
Contribution to journal
publication status
published
keywords
Apoptosis, EF hand calcium binding protein, Grancalcin, Penta-EF hand family protein, Protein-protein interaction, Sorcin
in
FEBS Letters
volume
545
issue
2-3
pages
4 pages
publisher
Wiley-Blackwell
external identifiers
  • scopus:0037526593
  • pmid:12804766
ISSN
0014-5793
DOI
10.1016/S0014-5793(03)00518-0
language
English
LU publication?
no
id
0e571fda-0cad-4b0f-bd4b-02c9f560a52f
date added to LUP
2018-09-11 15:00:49
date last changed
2025-10-14 11:28:21
@article{0e571fda-0cad-4b0f-bd4b-02c9f560a52f,
  abstract     = {{<p>The penta-EF hand (PEF) family of calcium binding proteins includes grancalcin, peflin, sorcin, calpain large and small subunits as well as ALG-2. Systematic testing of the heterodimerization abilities of the PEF proteins using the yeast two-hybrid and glutathione S-transferase pull-down assays revealed the new finding that grancalcin interacts strongly with sorcin. In addition, sorcin and grancalcin can be co-immunoprecipitated from lysates of human umbilical vein endothelial cells. Our results indicate that heterodimerization, in addition to differential interactions with target proteins, might be a way to regulate and fine tune processes mediated by calcium binding proteins of the penta-EF hand type.</p>}},
  author       = {{Hansen, Christian and Tarabykina, Svetlana and La Cour, Jonas M. and Lollike, Karsten and Berchtold, Martin W.}},
  issn         = {{0014-5793}},
  keywords     = {{Apoptosis; EF hand calcium binding protein; Grancalcin; Penta-EF hand family protein; Protein-protein interaction; Sorcin}},
  language     = {{eng}},
  month        = {{06}},
  number       = {{2-3}},
  pages        = {{151--154}},
  publisher    = {{Wiley-Blackwell}},
  series       = {{FEBS Letters}},
  title        = {{The PEF family proteins sorcin and grancalcin interact in vivo and in vitro}},
  url          = {{http://dx.doi.org/10.1016/S0014-5793(03)00518-0}},
  doi          = {{10.1016/S0014-5793(03)00518-0}},
  volume       = {{545}},
  year         = {{2003}},
}