The PEF family proteins sorcin and grancalcin interact in vivo and in vitro
(2003) In FEBS Letters 545(2-3). p.151-154- Abstract
The penta-EF hand (PEF) family of calcium binding proteins includes grancalcin, peflin, sorcin, calpain large and small subunits as well as ALG-2. Systematic testing of the heterodimerization abilities of the PEF proteins using the yeast two-hybrid and glutathione S-transferase pull-down assays revealed the new finding that grancalcin interacts strongly with sorcin. In addition, sorcin and grancalcin can be co-immunoprecipitated from lysates of human umbilical vein endothelial cells. Our results indicate that heterodimerization, in addition to differential interactions with target proteins, might be a way to regulate and fine tune processes mediated by calcium binding proteins of the penta-EF hand type.
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/0e571fda-0cad-4b0f-bd4b-02c9f560a52f
- author
- Hansen, Christian LU ; Tarabykina, Svetlana ; La Cour, Jonas M. ; Lollike, Karsten and Berchtold, Martin W.
- publishing date
- 2003-06-19
- type
- Contribution to journal
- publication status
- published
- keywords
- Apoptosis, EF hand calcium binding protein, Grancalcin, Penta-EF hand family protein, Protein-protein interaction, Sorcin
- in
- FEBS Letters
- volume
- 545
- issue
- 2-3
- pages
- 4 pages
- publisher
- Wiley-Blackwell
- external identifiers
-
- pmid:12804766
- scopus:0037526593
- ISSN
- 0014-5793
- DOI
- 10.1016/S0014-5793(03)00518-0
- language
- English
- LU publication?
- no
- id
- 0e571fda-0cad-4b0f-bd4b-02c9f560a52f
- date added to LUP
- 2018-09-11 15:00:49
- date last changed
- 2024-01-15 01:11:53
@article{0e571fda-0cad-4b0f-bd4b-02c9f560a52f, abstract = {{<p>The penta-EF hand (PEF) family of calcium binding proteins includes grancalcin, peflin, sorcin, calpain large and small subunits as well as ALG-2. Systematic testing of the heterodimerization abilities of the PEF proteins using the yeast two-hybrid and glutathione S-transferase pull-down assays revealed the new finding that grancalcin interacts strongly with sorcin. In addition, sorcin and grancalcin can be co-immunoprecipitated from lysates of human umbilical vein endothelial cells. Our results indicate that heterodimerization, in addition to differential interactions with target proteins, might be a way to regulate and fine tune processes mediated by calcium binding proteins of the penta-EF hand type.</p>}}, author = {{Hansen, Christian and Tarabykina, Svetlana and La Cour, Jonas M. and Lollike, Karsten and Berchtold, Martin W.}}, issn = {{0014-5793}}, keywords = {{Apoptosis; EF hand calcium binding protein; Grancalcin; Penta-EF hand family protein; Protein-protein interaction; Sorcin}}, language = {{eng}}, month = {{06}}, number = {{2-3}}, pages = {{151--154}}, publisher = {{Wiley-Blackwell}}, series = {{FEBS Letters}}, title = {{The PEF family proteins sorcin and grancalcin interact in vivo and in vitro}}, url = {{http://dx.doi.org/10.1016/S0014-5793(03)00518-0}}, doi = {{10.1016/S0014-5793(03)00518-0}}, volume = {{545}}, year = {{2003}}, }