Lipase-catalyzed alcoholysis of triglycerides for the preparation of 2-monoglycerides
(1994) In Enzyme and Microbial Technology 16(12). p.1042-1047- Abstract
Rhizopus arrhizus lipase immobilized on celite was used to prepare isomerically pure 2-monoglycerides by alcoholysis of triglycerides in organic media. Reaction parameters such as choice of solvent, choice of alcohol, and alcohol concentration were studied. When 12.5 mM tripalmitin was used as substrate, methyl-tert-butyl ether was the best solvent for alcoholysis at water activity 0.11. Ethanol gave the highest yield (97%) at an optimal ethanol concentration of 200-300 mM. At higher alcohol concentrations, the enzyme activity was substantially lowered. The enzyme preparation showed high stability in repeated-batch reactions.
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- author
- Millqvist, Anna
; Adlercreutz, Patrick
LU
and Mattiasson, Bo LU
- organization
- publishing date
- 1994-01-01
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- 2-monoglycerides, alcoholysis, hydrolysis, Lipase, operational stability, organic solvents, water activity
- in
- Enzyme and Microbial Technology
- volume
- 16
- issue
- 12
- pages
- 6 pages
- publisher
- Elsevier
- external identifiers
-
- scopus:0028657428
- ISSN
- 0141-0229
- DOI
- 10.1016/0141-0229(94)90140-6
- language
- English
- LU publication?
- yes
- id
- 0ec20a35-26ae-49e4-9afe-12b27a4e045d
- date added to LUP
- 2019-06-22 09:12:06
- date last changed
- 2021-01-03 04:15:42
@article{0ec20a35-26ae-49e4-9afe-12b27a4e045d, abstract = {{<p>Rhizopus arrhizus lipase immobilized on celite was used to prepare isomerically pure 2-monoglycerides by alcoholysis of triglycerides in organic media. Reaction parameters such as choice of solvent, choice of alcohol, and alcohol concentration were studied. When 12.5 mM tripalmitin was used as substrate, methyl-tert-butyl ether was the best solvent for alcoholysis at water activity 0.11. Ethanol gave the highest yield (97%) at an optimal ethanol concentration of 200-300 mM. At higher alcohol concentrations, the enzyme activity was substantially lowered. The enzyme preparation showed high stability in repeated-batch reactions.</p>}}, author = {{Millqvist, Anna and Adlercreutz, Patrick and Mattiasson, Bo}}, issn = {{0141-0229}}, keywords = {{2-monoglycerides; alcoholysis; hydrolysis; Lipase; operational stability; organic solvents; water activity}}, language = {{eng}}, month = {{01}}, number = {{12}}, pages = {{1042--1047}}, publisher = {{Elsevier}}, series = {{Enzyme and Microbial Technology}}, title = {{Lipase-catalyzed alcoholysis of triglycerides for the preparation of 2-monoglycerides}}, url = {{http://dx.doi.org/10.1016/0141-0229(94)90140-6}}, doi = {{10.1016/0141-0229(94)90140-6}}, volume = {{16}}, year = {{1994}}, }