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Lipase-catalyzed alcoholysis of triglycerides for the preparation of 2-monoglycerides

Millqvist, Anna ; Adlercreutz, Patrick LU orcid and Mattiasson, Bo LU (1994) In Enzyme and Microbial Technology 16(12). p.1042-1047
Abstract

Rhizopus arrhizus lipase immobilized on celite was used to prepare isomerically pure 2-monoglycerides by alcoholysis of triglycerides in organic media. Reaction parameters such as choice of solvent, choice of alcohol, and alcohol concentration were studied. When 12.5 mM tripalmitin was used as substrate, methyl-tert-butyl ether was the best solvent for alcoholysis at water activity 0.11. Ethanol gave the highest yield (97%) at an optimal ethanol concentration of 200-300 mM. At higher alcohol concentrations, the enzyme activity was substantially lowered. The enzyme preparation showed high stability in repeated-batch reactions.

Please use this url to cite or link to this publication:
author
; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
2-monoglycerides, alcoholysis, hydrolysis, Lipase, operational stability, organic solvents, water activity
in
Enzyme and Microbial Technology
volume
16
issue
12
pages
6 pages
publisher
Elsevier
external identifiers
  • scopus:0028657428
ISSN
0141-0229
DOI
10.1016/0141-0229(94)90140-6
language
English
LU publication?
yes
id
0ec20a35-26ae-49e4-9afe-12b27a4e045d
date added to LUP
2019-06-22 09:12:06
date last changed
2021-01-03 04:15:42
@article{0ec20a35-26ae-49e4-9afe-12b27a4e045d,
  abstract     = {{<p>Rhizopus arrhizus lipase immobilized on celite was used to prepare isomerically pure 2-monoglycerides by alcoholysis of triglycerides in organic media. Reaction parameters such as choice of solvent, choice of alcohol, and alcohol concentration were studied. When 12.5 mM tripalmitin was used as substrate, methyl-tert-butyl ether was the best solvent for alcoholysis at water activity 0.11. Ethanol gave the highest yield (97%) at an optimal ethanol concentration of 200-300 mM. At higher alcohol concentrations, the enzyme activity was substantially lowered. The enzyme preparation showed high stability in repeated-batch reactions.</p>}},
  author       = {{Millqvist, Anna and Adlercreutz, Patrick and Mattiasson, Bo}},
  issn         = {{0141-0229}},
  keywords     = {{2-monoglycerides; alcoholysis; hydrolysis; Lipase; operational stability; organic solvents; water activity}},
  language     = {{eng}},
  month        = {{01}},
  number       = {{12}},
  pages        = {{1042--1047}},
  publisher    = {{Elsevier}},
  series       = {{Enzyme and Microbial Technology}},
  title        = {{Lipase-catalyzed alcoholysis of triglycerides for the preparation of 2-monoglycerides}},
  url          = {{http://dx.doi.org/10.1016/0141-0229(94)90140-6}},
  doi          = {{10.1016/0141-0229(94)90140-6}},
  volume       = {{16}},
  year         = {{1994}},
}