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Plasmodium falciparum : a simplified technique for obtaining singly infected erythrocytes

Puthia, Manoj K LU and Tan, Kevin S W (2005) In Parasitology Research 95(3). p.8-176
Abstract

We report the development of a simple technique involving 15 ml polypropylene tubes and a rotatory incubator for obtaining erythrocytes singly infected with Plasmodium falciparum. This technique will be useful for cloning of the parasite. Our finding that P. falciparum merozoite invasion is inhibited during rotation suggests that this method may also be useful for the study of parasite-erythrocyte interactions under dynamic circulatory conditions.

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author
publishing date
type
Contribution to journal
publication status
published
keywords
Animals, Culture Media, Erythrocytes/cytology, Humans, Malaria, Falciparum/parasitology, Parasitemia/parasitology, Parasitology/instrumentation, Plasmodium falciparum/growth & development, Polypropylenes
in
Parasitology Research
volume
95
issue
3
pages
8 - 176
publisher
Springer
external identifiers
  • scopus:15044345186
ISSN
0932-0113
DOI
10.1007/s00436-004-1266-6
language
English
LU publication?
no
id
0ed015ae-d5ed-4b79-84b9-516a4957bc7b
date added to LUP
2018-08-27 13:49:58
date last changed
2019-02-20 11:24:49
@article{0ed015ae-d5ed-4b79-84b9-516a4957bc7b,
  abstract     = {<p>We report the development of a simple technique involving 15 ml polypropylene tubes and a rotatory incubator for obtaining erythrocytes singly infected with Plasmodium falciparum. This technique will be useful for cloning of the parasite. Our finding that P. falciparum merozoite invasion is inhibited during rotation suggests that this method may also be useful for the study of parasite-erythrocyte interactions under dynamic circulatory conditions.</p>},
  author       = {Puthia, Manoj K and Tan, Kevin S W},
  issn         = {0932-0113},
  keyword      = {Animals,Culture Media,Erythrocytes/cytology,Humans,Malaria, Falciparum/parasitology,Parasitemia/parasitology,Parasitology/instrumentation,Plasmodium falciparum/growth & development,Polypropylenes},
  language     = {eng},
  number       = {3},
  pages        = {8--176},
  publisher    = {Springer},
  series       = {Parasitology Research},
  title        = {Plasmodium falciparum : a simplified technique for obtaining singly infected erythrocytes},
  url          = {http://dx.doi.org/10.1007/s00436-004-1266-6},
  volume       = {95},
  year         = {2005},
}