Diagnostic PCR: validation and sample preparation are two sides of the same coin
(2004) In APMIS : acta pathologica, microbiologica, et immunologica Scandinavica 112(11-12). p.808-814- Abstract
- Increased use of powerful PCR technology for the routine detection of pathogens has focused attention on the need for international validation and preparation of official non-commercial guidelines. Bacteria of epidemiological importance should be the prime focus, although a "validation infrastructure" once established could easily be adapted for PCR-based detection of viruses and parasites. The aim of standardization should be the widespread adoption of diagnostic PCR for routine pathogen testing. European experience provides the impetus for realization of this vision through preparation of quantitative reference DNA material and reagents, production of stringent protocols and tools for thermal cycler performance testing, uncomplicated... (More)
- Increased use of powerful PCR technology for the routine detection of pathogens has focused attention on the need for international validation and preparation of official non-commercial guidelines. Bacteria of epidemiological importance should be the prime focus, although a "validation infrastructure" once established could easily be adapted for PCR-based detection of viruses and parasites. The aim of standardization should be the widespread adoption of diagnostic PCR for routine pathogen testing. European experience provides the impetus for realization of this vision through preparation of quantitative reference DNA material and reagents, production of stringent protocols and tools for thermal cycler performance testing, uncomplicated sample preparation techniques, and extensive ring trials for assessment of the efficacy of selected matrix/pathogen detection protocols. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/141750
- author
- Hoorfar, Jeffrey ; Wolffs, Petra LU and Rådström, Peter LU
- organization
- publishing date
- 2004
- type
- Contribution to journal
- publication status
- published
- subject
- in
- APMIS : acta pathologica, microbiologica, et immunologica Scandinavica
- volume
- 112
- issue
- 11-12
- pages
- 808 - 814
- publisher
- John Wiley & Sons Inc.
- external identifiers
-
- wos:000226305700007
- scopus:12744272142
- ISSN
- 1600-0463
- DOI
- 10.1111/j.1600-0463.2004.apm11211-1207.x
- language
- English
- LU publication?
- yes
- id
- 0f853823-0d22-485b-93b7-820a8b0d5779 (old id 141750)
- date added to LUP
- 2016-04-01 12:04:39
- date last changed
- 2022-04-29 00:17:57
@article{0f853823-0d22-485b-93b7-820a8b0d5779,
abstract = {{Increased use of powerful PCR technology for the routine detection of pathogens has focused attention on the need for international validation and preparation of official non-commercial guidelines. Bacteria of epidemiological importance should be the prime focus, although a "validation infrastructure" once established could easily be adapted for PCR-based detection of viruses and parasites. The aim of standardization should be the widespread adoption of diagnostic PCR for routine pathogen testing. European experience provides the impetus for realization of this vision through preparation of quantitative reference DNA material and reagents, production of stringent protocols and tools for thermal cycler performance testing, uncomplicated sample preparation techniques, and extensive ring trials for assessment of the efficacy of selected matrix/pathogen detection protocols.}},
author = {{Hoorfar, Jeffrey and Wolffs, Petra and Rådström, Peter}},
issn = {{1600-0463}},
language = {{eng}},
number = {{11-12}},
pages = {{808--814}},
publisher = {{John Wiley & Sons Inc.}},
series = {{APMIS : acta pathologica, microbiologica, et immunologica Scandinavica}},
title = {{Diagnostic PCR: validation and sample preparation are two sides of the same coin}},
url = {{http://dx.doi.org/10.1111/j.1600-0463.2004.apm11211-1207.x}},
doi = {{10.1111/j.1600-0463.2004.apm11211-1207.x}},
volume = {{112}},
year = {{2004}},
}