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Evaluation of simple enzyme kinetics by response surface modelling

Andersson, Mats LU and Adlercreutz, Patrick LU (1999) In Biotechnology Techniques 13(12). p.903-907
Abstract

The activity of a horse liver alcohol dehydrogenase catalysed reduction of cyclohexanone was investigated by using a central composite circumscribed design in which two parameters (pH and cyclohexanone concentration) were varied. By log transformation of the substrate concentration an adequate model could be obtained from which reliable kinetic constants and pH profiles were determined.

Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Alcohol dehydrogenase, Cyclohexanone, Enzyme kinetics, Response surface modelling
in
Biotechnology Techniques
volume
13
issue
12
pages
5 pages
publisher
Springer
external identifiers
  • scopus:0033380112
ISSN
0951-208X
DOI
10.1023/A:1008994613645
language
English
LU publication?
yes
id
0fabffbc-2325-4b00-b2bb-67c96b13e65b
date added to LUP
2019-06-20 15:21:44
date last changed
2020-01-13 02:04:01
@article{0fabffbc-2325-4b00-b2bb-67c96b13e65b,
  abstract     = {<p>The activity of a horse liver alcohol dehydrogenase catalysed reduction of cyclohexanone was investigated by using a central composite circumscribed design in which two parameters (pH and cyclohexanone concentration) were varied. By log transformation of the substrate concentration an adequate model could be obtained from which reliable kinetic constants and pH profiles were determined.</p>},
  author       = {Andersson, Mats and Adlercreutz, Patrick},
  issn         = {0951-208X},
  language     = {eng},
  month        = {12},
  number       = {12},
  pages        = {903--907},
  publisher    = {Springer},
  series       = {Biotechnology Techniques},
  title        = {Evaluation of simple enzyme kinetics by response surface modelling},
  url          = {http://dx.doi.org/10.1023/A:1008994613645},
  doi          = {10.1023/A:1008994613645},
  volume       = {13},
  year         = {1999},
}