Evaluation of simple enzyme kinetics by response surface modelling
(1999) In Biotechnology Techniques 13(12). p.903-907- Abstract
The activity of a horse liver alcohol dehydrogenase catalysed reduction of cyclohexanone was investigated by using a central composite circumscribed design in which two parameters (pH and cyclohexanone concentration) were varied. By log transformation of the substrate concentration an adequate model could be obtained from which reliable kinetic constants and pH profiles were determined.
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https://lup.lub.lu.se/record/0fabffbc-2325-4b00-b2bb-67c96b13e65b
- author
- Andersson, Mats LU and Adlercreutz, Patrick LU
- organization
- publishing date
- 1999-12-01
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Alcohol dehydrogenase, Cyclohexanone, Enzyme kinetics, Response surface modelling
- in
- Biotechnology Techniques
- volume
- 13
- issue
- 12
- pages
- 5 pages
- publisher
- Springer
- external identifiers
-
- scopus:0033380112
- ISSN
- 0951-208X
- DOI
- 10.1023/A:1008994613645
- language
- English
- LU publication?
- yes
- id
- 0fabffbc-2325-4b00-b2bb-67c96b13e65b
- date added to LUP
- 2019-06-20 15:21:44
- date last changed
- 2022-01-31 22:13:19
@article{0fabffbc-2325-4b00-b2bb-67c96b13e65b, abstract = {{<p>The activity of a horse liver alcohol dehydrogenase catalysed reduction of cyclohexanone was investigated by using a central composite circumscribed design in which two parameters (pH and cyclohexanone concentration) were varied. By log transformation of the substrate concentration an adequate model could be obtained from which reliable kinetic constants and pH profiles were determined.</p>}}, author = {{Andersson, Mats and Adlercreutz, Patrick}}, issn = {{0951-208X}}, keywords = {{Alcohol dehydrogenase; Cyclohexanone; Enzyme kinetics; Response surface modelling}}, language = {{eng}}, month = {{12}}, number = {{12}}, pages = {{903--907}}, publisher = {{Springer}}, series = {{Biotechnology Techniques}}, title = {{Evaluation of simple enzyme kinetics by response surface modelling}}, url = {{http://dx.doi.org/10.1023/A:1008994613645}}, doi = {{10.1023/A:1008994613645}}, volume = {{13}}, year = {{1999}}, }