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Enteral Pancreatic-like Enzymes of Microbial Origin affect Insulin Release during an Intravenous Glucose Tolerance Test

Pierzynowski, Stefan LU ; Goncharova, Katerina LU ; Wolinski, J; Prykhodko, Olena LU ; Weström, Björn LU and Lozinska, Liudmyla LU (2016) In Journal of Diabetes & Metabolism 7(6).
Abstract
We have previously shown that the presence of pancreatic enzymes in the gut lumen of exocrine pancreatic insufficient pigs influences blood glucose and insulin levels during an intravenous glucose tolerance test (IVGTT). The present study aims to highlight the effects of orally applied pancreatic-like enzymes on blood glucose and plasma insulin levels during an IVGTT in young intact pigs. Five, 7-week old pigs were fed with pancreatic-like enzymes of microbial origin, a proteinase (from Aspergillus melleus), α-amylase (from Aspergillus oryzae) or lipase (from Burkholderia cepacia) alone or in combination with the Ca/Na salts of α-ketoglutaric acid (AKG). One hour following administration of the various supplements an IVGTT was performed.... (More)
We have previously shown that the presence of pancreatic enzymes in the gut lumen of exocrine pancreatic insufficient pigs influences blood glucose and insulin levels during an intravenous glucose tolerance test (IVGTT). The present study aims to highlight the effects of orally applied pancreatic-like enzymes on blood glucose and plasma insulin levels during an IVGTT in young intact pigs. Five, 7-week old pigs were fed with pancreatic-like enzymes of microbial origin, a proteinase (from Aspergillus melleus), α-amylase (from Aspergillus oryzae) or lipase (from Burkholderia cepacia) alone or in combination with the Ca/Na salts of α-ketoglutaric acid (AKG). One hour following administration of the various supplements an IVGTT was performed. Blood samples were withdrawn during the 2 hours of IVGTT for glucose and insulin analyses. Blood glucose during the IVGTT was identical following administration of all combinations of the various enzymes or enzyme mixtures. Enteral loading of amylase or any amylase containing mixture resulted in reduced insulin secretion while administration of proteinase or any proteinase containing mixture resulted in enhanced insulin secretion during IVGTT, as compared to the control water vehicle. Lipase or AKG and lipase or AKG containing mixtures did not affect insulin secretion. Thus, it can be suggested that host amylase/protease ratio and their amount in pancreatic juice can participate in regulation of insulin release, thus, possibly affecting development of obesity and diabetes type 2. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
α-amylase, Proteinase
in
Journal of Diabetes & Metabolism
volume
7
issue
6
pages
8 pages
external identifiers
  • wos:000396374900006
ISSN
2155-6156
DOI
10.4172/2155-6156.1000681
language
English
LU publication?
yes
id
0fb0f8c4-1bd6-4967-bd2b-0fe686dc8054
alternative location
https://www.omicsonline.org/open-access/enteral-pancreaticlike-enzymes-of-microbial-origin-affect-insulin-releaseduring-an-intravenous-glucose-tolerance-test-2155-6156-1000681.php?aid=75969
date added to LUP
2017-06-15 16:18:33
date last changed
2017-10-23 13:57:15
@article{0fb0f8c4-1bd6-4967-bd2b-0fe686dc8054,
  abstract     = {We have previously shown that the presence of pancreatic enzymes in the gut lumen of exocrine pancreatic insufficient pigs influences blood glucose and insulin levels during an intravenous glucose tolerance test (IVGTT). The present study aims to highlight the effects of orally applied pancreatic-like enzymes on blood glucose and plasma insulin levels during an IVGTT in young intact pigs. Five, 7-week old pigs were fed with pancreatic-like enzymes of microbial origin, a proteinase (from Aspergillus melleus), α-amylase (from Aspergillus oryzae) or lipase (from Burkholderia cepacia) alone or in combination with the Ca/Na salts of α-ketoglutaric acid (AKG). One hour following administration of the various supplements an IVGTT was performed. Blood samples were withdrawn during the 2 hours of IVGTT for glucose and insulin analyses. Blood glucose during the IVGTT was identical following administration of all combinations of the various enzymes or enzyme mixtures. Enteral loading of amylase or any amylase containing mixture resulted in reduced insulin secretion while administration of proteinase or any proteinase containing mixture resulted in enhanced insulin secretion during IVGTT, as compared to the control water vehicle. Lipase or AKG and lipase or AKG containing mixtures did not affect insulin secretion. Thus, it can be suggested that host amylase/protease ratio and their amount in pancreatic juice can participate in regulation of insulin release, thus, possibly affecting development of obesity and diabetes type 2.},
  articleno    = {681},
  author       = {Pierzynowski, Stefan and Goncharova, Katerina and Wolinski, J and Prykhodko, Olena and Weström, Björn and Lozinska, Liudmyla},
  issn         = {2155-6156},
  keyword      = {α-amylase,Proteinase},
  language     = {eng},
  month        = {06},
  number       = {6},
  pages        = {8},
  series       = {Journal of Diabetes & Metabolism},
  title        = {Enteral Pancreatic-like Enzymes of Microbial Origin affect Insulin Release during an Intravenous Glucose Tolerance Test},
  url          = {http://dx.doi.org/10.4172/2155-6156.1000681},
  volume       = {7},
  year         = {2016},
}