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Placental expression profiling in preeclampsia: local overproduction of hemoglobin may drive pathological changes.

Centlow, Magnus LU ; Carninci, Piero; Nemeth, Krisztian; Mezey, Eva; Brownstein, Michael and Hansson, Stefan LU (2008) In Fertility and Sterility 90. p.1834-1843
Abstract
OBJECTIVE: To create a library enriched in cDNAs from preeclamptic placentas to print onto microarrays for placental profiling of preeclampsia (PE) and high risk pregnancies. DESIGN: Prospective study. SETTING: University women's clinic and academic research laboratory. PATIENT(S): Ten patients with PE, 5 with PE and bilateral notching, 5 with bilateral notching without PE, and 15 normotensive patients were recruited. INTERVENTION(S): Placenta and placenta bed biopsies were collected after delivery. MAIN OUTCOME MEASURE(S): Subtracted libraries of PE transcripts were produced, and cDNAs from these libraries were used to make PE-specific cDNA arrays. Results were verified quantitatively using real-time polymerase chain reaction (PCR) and... (More)
OBJECTIVE: To create a library enriched in cDNAs from preeclamptic placentas to print onto microarrays for placental profiling of preeclampsia (PE) and high risk pregnancies. DESIGN: Prospective study. SETTING: University women's clinic and academic research laboratory. PATIENT(S): Ten patients with PE, 5 with PE and bilateral notching, 5 with bilateral notching without PE, and 15 normotensive patients were recruited. INTERVENTION(S): Placenta and placenta bed biopsies were collected after delivery. MAIN OUTCOME MEASURE(S): Subtracted libraries of PE transcripts were produced, and cDNAs from these libraries were used to make PE-specific cDNA arrays. Results were verified quantitatively using real-time polymerase chain reaction (PCR) and histologically using in situ hybridization and immunohistochemistry. RESULT(S): Thirty genes were significantly altered in at least one group comparison. Differences in two candidate genes were confirmed using quantitative real-time PCR. Hemoglobin alpha2 and gamma transcripts were significantly overexpressed in the PE placenta. Scattered cells in the placenta and placental blood vessels were shown to express genes encoding these hemoglobin chains. CONCLUSION(S): We demonstrate increased hemoglobin production in the PE placenta. The hemoglobin may be released into the placenta blood vessel lumen. Free heme and hemoglobin are potent toxins that cause endothelial damage and inflammation. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Fertility and Sterility
volume
90
pages
1834 - 1843
publisher
Elsevier
external identifiers
  • pmid:18166190
  • wos:000260752000040
  • scopus:55349132052
ISSN
1556-5653
DOI
10.1016/j.fertnstert.2007.09.030
language
English
LU publication?
yes
id
073fa524-86a7-4ed6-b8b7-a312c24b31aa (old id 1021722)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/18166190?dopt=Abstract
date added to LUP
2008-02-13 15:32:56
date last changed
2017-07-23 04:52:16
@article{073fa524-86a7-4ed6-b8b7-a312c24b31aa,
  abstract     = {OBJECTIVE: To create a library enriched in cDNAs from preeclamptic placentas to print onto microarrays for placental profiling of preeclampsia (PE) and high risk pregnancies. DESIGN: Prospective study. SETTING: University women's clinic and academic research laboratory. PATIENT(S): Ten patients with PE, 5 with PE and bilateral notching, 5 with bilateral notching without PE, and 15 normotensive patients were recruited. INTERVENTION(S): Placenta and placenta bed biopsies were collected after delivery. MAIN OUTCOME MEASURE(S): Subtracted libraries of PE transcripts were produced, and cDNAs from these libraries were used to make PE-specific cDNA arrays. Results were verified quantitatively using real-time polymerase chain reaction (PCR) and histologically using in situ hybridization and immunohistochemistry. RESULT(S): Thirty genes were significantly altered in at least one group comparison. Differences in two candidate genes were confirmed using quantitative real-time PCR. Hemoglobin alpha2 and gamma transcripts were significantly overexpressed in the PE placenta. Scattered cells in the placenta and placental blood vessels were shown to express genes encoding these hemoglobin chains. CONCLUSION(S): We demonstrate increased hemoglobin production in the PE placenta. The hemoglobin may be released into the placenta blood vessel lumen. Free heme and hemoglobin are potent toxins that cause endothelial damage and inflammation.},
  author       = {Centlow, Magnus and Carninci, Piero and Nemeth, Krisztian and Mezey, Eva and Brownstein, Michael and Hansson, Stefan},
  issn         = {1556-5653},
  language     = {eng},
  pages        = {1834--1843},
  publisher    = {Elsevier},
  series       = {Fertility and Sterility},
  title        = {Placental expression profiling in preeclampsia: local overproduction of hemoglobin may drive pathological changes.},
  url          = {http://dx.doi.org/10.1016/j.fertnstert.2007.09.030},
  volume       = {90},
  year         = {2008},
}