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DNA of Helicobacter spp. and common gut bacteria in primary liver carcinoma.

Abu Al-Soud, Waleed LU ; Stenram, Unne LU ; Ljungh, Åsa LU ; Tranberg, Karl-Göran LU ; Nilsson, Hans-Olof LU and Wadström, Torkel LU (2008) In Digestive and Liver Disease 40(2). p.126-131
Abstract
BACKGROUND AND AIM: Gastric and enteric Helicobacter species have been associated with the pathogenesis of some extragastric diseases. METHODS: We retrospectively investigated the presence of DNA of Helicobacter species in samples of the cancer and the surrounding tumour-free liver tissues of patients with hepatocellular carcinoma (HCC, n=12) and cholangiocarcinoma (CC, n=13). The patients were from an area with low liver cancer incidence and with low hepatitis B and C prevalence. Patients with a benign liver disease (n=24) were included as controls. Paraffin-embedded liver samples were examined by a Helicobacter genus-specific PCR assay as well as group-specific PCR assays for Enterobacteriaceae, Bacteroides, Lactobacillus and... (More)
BACKGROUND AND AIM: Gastric and enteric Helicobacter species have been associated with the pathogenesis of some extragastric diseases. METHODS: We retrospectively investigated the presence of DNA of Helicobacter species in samples of the cancer and the surrounding tumour-free liver tissues of patients with hepatocellular carcinoma (HCC, n=12) and cholangiocarcinoma (CC, n=13). The patients were from an area with low liver cancer incidence and with low hepatitis B and C prevalence. Patients with a benign liver disease (n=24) were included as controls. Paraffin-embedded liver samples were examined by a Helicobacter genus-specific PCR assay as well as group-specific PCR assays for Enterobacteriaceae, Bacteroides, Lactobacillus and Enterococcus. PCR products of positive samples were characterised by denaturing gradient gel electrophoresis (DGGE) and DNA sequencing. RESULTS: PCR assay detected Helicobacter DNA in seven of 12 (58%) and eight of 13 (62%) normal liver tissue specimens from HCC and CC patients, respectively. Two cancer samples from HCC patients were Helicobacter-positive but none of the CC cancers. In the control group, three of 24 (12.5%) patients with a benign liver condition were positive for Helicobacter species (p<0.01 compared to results of tumour-free liver tissue from the cancer patients). DGGE and DNA sequence analysis showed that 90% of the detected PCR products were "H. pylori-like". DNA of some other enteric bacteria was detected in the liver of one cancer patient and one control (4% of all patients). CONCLUSION: The presence of DNA of Helicobacter species in liver specimens, but not of other common gut bacteria, was associated with human hepatic carcinogenesis. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Digestive and Liver Disease
volume
40
issue
2
pages
126 - 131
publisher
Elsevier
external identifiers
  • pmid:18083084
  • wos:000253402800008
  • scopus:38049062584
ISSN
1590-8658
DOI
10.1016/j.dld.2007.09.011
language
English
LU publication?
yes
id
c3c38bb4-6d01-459e-ba0a-a7d307ea379c (old id 1035195)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/18083084?dopt=Abstract
date added to LUP
2008-02-21 14:54:13
date last changed
2017-11-05 04:43:56
@article{c3c38bb4-6d01-459e-ba0a-a7d307ea379c,
  abstract     = {BACKGROUND AND AIM: Gastric and enteric Helicobacter species have been associated with the pathogenesis of some extragastric diseases. METHODS: We retrospectively investigated the presence of DNA of Helicobacter species in samples of the cancer and the surrounding tumour-free liver tissues of patients with hepatocellular carcinoma (HCC, n=12) and cholangiocarcinoma (CC, n=13). The patients were from an area with low liver cancer incidence and with low hepatitis B and C prevalence. Patients with a benign liver disease (n=24) were included as controls. Paraffin-embedded liver samples were examined by a Helicobacter genus-specific PCR assay as well as group-specific PCR assays for Enterobacteriaceae, Bacteroides, Lactobacillus and Enterococcus. PCR products of positive samples were characterised by denaturing gradient gel electrophoresis (DGGE) and DNA sequencing. RESULTS: PCR assay detected Helicobacter DNA in seven of 12 (58%) and eight of 13 (62%) normal liver tissue specimens from HCC and CC patients, respectively. Two cancer samples from HCC patients were Helicobacter-positive but none of the CC cancers. In the control group, three of 24 (12.5%) patients with a benign liver condition were positive for Helicobacter species (p&lt;0.01 compared to results of tumour-free liver tissue from the cancer patients). DGGE and DNA sequence analysis showed that 90% of the detected PCR products were "H. pylori-like". DNA of some other enteric bacteria was detected in the liver of one cancer patient and one control (4% of all patients). CONCLUSION: The presence of DNA of Helicobacter species in liver specimens, but not of other common gut bacteria, was associated with human hepatic carcinogenesis.},
  author       = {Abu Al-Soud, Waleed and Stenram, Unne and Ljungh, Åsa and Tranberg, Karl-Göran and Nilsson, Hans-Olof and Wadström, Torkel},
  issn         = {1590-8658},
  language     = {eng},
  number       = {2},
  pages        = {126--131},
  publisher    = {Elsevier},
  series       = {Digestive and Liver Disease},
  title        = {DNA of Helicobacter spp. and common gut bacteria in primary liver carcinoma.},
  url          = {http://dx.doi.org/10.1016/j.dld.2007.09.011},
  volume       = {40},
  year         = {2008},
}