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Alpha10 integrin expression is up-regulated on fibroblast growth factor-2-treated mesenchymal stem cells with improved chondrogenic differentiation potential.

Varas, Laura; Ohlsson, Lars Bryngelson; Honeth, Gabriella LU ; Olsson, Andreas; Bengtsson, Therese; Wiberg, Charlotte; Bockermann, Robert; Järnum, Sofia; Richter, Johan LU and Pennington, Douglas, et al. (2007) In Stem Cells and Development 16(6). p.965-978
Abstract
Mesenchymal stem cells (MSCs) are multipotent cells that have the capacity to differentiate into various different cell lineages and can generate bone, cartilage and adipose tissue. MSCs are presently characterized using a broad range of different cell-surface markers that are not exclusive to MSCs and not sensitive to culture conditions or differentiation capacity. We show that the integrin subunits alpha10 and alpha11 of the collagen binding integrins alpha10beta1 and alpha11beta1 are expressed by human MSCs in monolayer cultures. We also demonstrate that the expression of alpha10 increases, while alpha1 and alpha11 decrease, during aggregate culture of MSCs in chondrogenic medium. Alpha10beta1 is expressed by chondrocytes in cartilage,... (More)
Mesenchymal stem cells (MSCs) are multipotent cells that have the capacity to differentiate into various different cell lineages and can generate bone, cartilage and adipose tissue. MSCs are presently characterized using a broad range of different cell-surface markers that are not exclusive to MSCs and not sensitive to culture conditions or differentiation capacity. We show that the integrin subunits alpha10 and alpha11 of the collagen binding integrins alpha10beta1 and alpha11beta1 are expressed by human MSCs in monolayer cultures. We also demonstrate that the expression of alpha10 increases, while alpha1 and alpha11 decrease, during aggregate culture of MSCs in chondrogenic medium. Alpha10beta1 is expressed by chondrocytes in cartilage, whereas alpha11beta1 integrins are predominantly expressed by subsets of the fibroblastic lineage. In extensive monolayer cultures of MSCs, alpha10 expression is down-regulated. We show that this down-regulation is reversed by fibroblast growth factor-2 (FGF-2) treatment. Addition of FGF-2 to MSCs not only results in increased alpha10 expression, but also in decreased alpha11 expression. FGF-2 treatment of MSCs has been shown to keep the cells more multipotent and also induces cell proliferation and Sox-9 up-regulation. We demonstrate improved chondrogenecity as well as increased collagen-dependant migratory potential of FGF-2-treated MSCs having a high alpha10 expression. We also demonstrate expression of alpha10 and alpha11 integrin subunits in the endosteum and periosteum of mice, but very low or not detectable expression levels in freshly aspired human or mouse BM. We show that MSCs with high chondrogenic differentiation potential are highly alpha10 positive and propose alpha10 as a potential marker to predict the differentiation state of MSCs. (Less)
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Contribution to journal
publication status
published
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Stem Cells and Development
volume
16
issue
6
pages
965 - 978
publisher
Mary Ann Liebert, Inc.
external identifiers
  • pmid:18047418
  • wos:000252031000009
  • scopus:37549019623
ISSN
1557-8534
DOI
10.1089/scd.2007.0049
language
English
LU publication?
yes
id
402a3022-d8a0-4dad-9d8c-d1ce5bc6aed4 (old id 1035802)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/18047418?dopt=Abstract
date added to LUP
2009-07-08 15:40:36
date last changed
2017-11-05 03:46:04
@article{402a3022-d8a0-4dad-9d8c-d1ce5bc6aed4,
  abstract     = {Mesenchymal stem cells (MSCs) are multipotent cells that have the capacity to differentiate into various different cell lineages and can generate bone, cartilage and adipose tissue. MSCs are presently characterized using a broad range of different cell-surface markers that are not exclusive to MSCs and not sensitive to culture conditions or differentiation capacity. We show that the integrin subunits alpha10 and alpha11 of the collagen binding integrins alpha10beta1 and alpha11beta1 are expressed by human MSCs in monolayer cultures. We also demonstrate that the expression of alpha10 increases, while alpha1 and alpha11 decrease, during aggregate culture of MSCs in chondrogenic medium. Alpha10beta1 is expressed by chondrocytes in cartilage, whereas alpha11beta1 integrins are predominantly expressed by subsets of the fibroblastic lineage. In extensive monolayer cultures of MSCs, alpha10 expression is down-regulated. We show that this down-regulation is reversed by fibroblast growth factor-2 (FGF-2) treatment. Addition of FGF-2 to MSCs not only results in increased alpha10 expression, but also in decreased alpha11 expression. FGF-2 treatment of MSCs has been shown to keep the cells more multipotent and also induces cell proliferation and Sox-9 up-regulation. We demonstrate improved chondrogenecity as well as increased collagen-dependant migratory potential of FGF-2-treated MSCs having a high alpha10 expression. We also demonstrate expression of alpha10 and alpha11 integrin subunits in the endosteum and periosteum of mice, but very low or not detectable expression levels in freshly aspired human or mouse BM. We show that MSCs with high chondrogenic differentiation potential are highly alpha10 positive and propose alpha10 as a potential marker to predict the differentiation state of MSCs.},
  author       = {Varas, Laura and Ohlsson, Lars Bryngelson and Honeth, Gabriella and Olsson, Andreas and Bengtsson, Therese and Wiberg, Charlotte and Bockermann, Robert and Järnum, Sofia and Richter, Johan and Pennington, Douglas and Johnstone, Brian and Lundgren-Akerlund, Evy and Kjellman, Christian},
  issn         = {1557-8534},
  language     = {eng},
  number       = {6},
  pages        = {965--978},
  publisher    = {Mary Ann Liebert, Inc.},
  series       = {Stem Cells and Development},
  title        = {Alpha10 integrin expression is up-regulated on fibroblast growth factor-2-treated mesenchymal stem cells with improved chondrogenic differentiation potential.},
  url          = {http://dx.doi.org/10.1089/scd.2007.0049},
  volume       = {16},
  year         = {2007},
}