Independent measures of insulin secretion and insulin sensitivity during the same test: the glucagon-insulin tolerance test.
Dorkhan, Mozhgan LU ; Tripathy, Devjit LU ; Malm, G ; Asgharian, H ; Tuomi, Tiinamaija LU
and Groop, Leif
LU
(2008)
In Journal of Internal Medicine
264.
p.62-71
- Abstract
- Background. To validate a test for independent assessment of insulin secretion and insulin sensitivity during the same occasion for metabolic studies in clinical practice, i.e. combined glucagon-stimulated C-peptide test and insulin tolerance test (GITT). Subjects and methods. We measured C-peptide response to 0.5 mg of intravenous glucagon followed 30 min later by administration of 0.05 U kg(-1) insulin (insulin tolerance test, ITT). Ten subjects with normal glucose tolerance participated on different days in an ITT, glucagon-C-peptide test, ITT followed by glucagon-C-peptide test and glucagon-C-peptide test followed by ITT to establish whether and how the tests could be combined. The test was then repeated in nine patients with type 2... (More)
- Background. To validate a test for independent assessment of insulin secretion and insulin sensitivity during the same occasion for metabolic studies in clinical practice, i.e. combined glucagon-stimulated C-peptide test and insulin tolerance test (GITT). Subjects and methods. We measured C-peptide response to 0.5 mg of intravenous glucagon followed 30 min later by administration of 0.05 U kg(-1) insulin (insulin tolerance test, ITT). Ten subjects with normal glucose tolerance participated on different days in an ITT, glucagon-C-peptide test, ITT followed by glucagon-C-peptide test and glucagon-C-peptide test followed by ITT to establish whether and how the tests could be combined. The test was then repeated in nine patients with type 2 diabetes to investigate its reproducibility. In 20 subjects with varying degrees of glucose tolerance, the test was compared with the Botnia clamp (an intravenous glucose tolerance test combined with a euglycaemic hyperinsulinemic clamp). Results. When ITT preceded the glucagon test, C-peptide response was blunted. Therefore, we first administered glucagon and then insulin (GITT). The K(ITT) from the GITT was reproducible (CV = 13 %) and correlated strongly with the glucose disposal rate from the Botnia clamp (r = 0.87, r(2) = 0.75, P < 0.001). The C-peptide response to glucagon was reproducible (CV = 13 %). The disposition index, providing a measure of beta-cell function adjusted for insulin sensitivity, calculated from the GITT showed good discrimination between individuals with varying degrees of glucose tolerance. Conclusions. The GITT provides simple, reproducible and independent estimates of insulin sensitivity and secretion on the same occasion for metabolic studies in individuals with normal and abnormal glucose tolerance. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1041552
- author
- Dorkhan, Mozhgan
LU
; Tripathy, Devjit
LU
; Malm, G
; Asgharian, H
; Tuomi, Tiinamaija
LU
and Groop, Leif
LU
- organization
- publishing date
- 2008
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Journal of Internal Medicine
- volume
- 264
- pages
- 62 - 71
- publisher
- Wiley-Blackwell
- external identifiers
-
- pmid:18298489
- wos:000256619200007
- scopus:44949174021
- pmid:18298489
- ISSN
- 1365-2796
- DOI
- 10.1111/j.1365-2796.2008.01921.x
- language
- English
- LU publication?
- yes
- additional info
- The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Diabetes and Endocrinology (013241530), Pediatrics/Urology/Gynecology/Endocrinology (013240400)
- id
- 22d20f8e-a608-4a48-9fff-fe30b47e3ffd (old id 1041552)
- alternative location
- http://www.ncbi.nlm.nih.gov/pubmed/18298489?dopt=Abstract
- date added to LUP
- 2016-04-04 09:29:50
- date last changed
- 2024-01-12 14:18:16
@article{22d20f8e-a608-4a48-9fff-fe30b47e3ffd,
abstract = {{Background. To validate a test for independent assessment of insulin secretion and insulin sensitivity during the same occasion for metabolic studies in clinical practice, i.e. combined glucagon-stimulated C-peptide test and insulin tolerance test (GITT). Subjects and methods. We measured C-peptide response to 0.5 mg of intravenous glucagon followed 30 min later by administration of 0.05 U kg(-1) insulin (insulin tolerance test, ITT). Ten subjects with normal glucose tolerance participated on different days in an ITT, glucagon-C-peptide test, ITT followed by glucagon-C-peptide test and glucagon-C-peptide test followed by ITT to establish whether and how the tests could be combined. The test was then repeated in nine patients with type 2 diabetes to investigate its reproducibility. In 20 subjects with varying degrees of glucose tolerance, the test was compared with the Botnia clamp (an intravenous glucose tolerance test combined with a euglycaemic hyperinsulinemic clamp). Results. When ITT preceded the glucagon test, C-peptide response was blunted. Therefore, we first administered glucagon and then insulin (GITT). The K(ITT) from the GITT was reproducible (CV = 13 %) and correlated strongly with the glucose disposal rate from the Botnia clamp (r = 0.87, r(2) = 0.75, P < 0.001). The C-peptide response to glucagon was reproducible (CV = 13 %). The disposition index, providing a measure of beta-cell function adjusted for insulin sensitivity, calculated from the GITT showed good discrimination between individuals with varying degrees of glucose tolerance. Conclusions. The GITT provides simple, reproducible and independent estimates of insulin sensitivity and secretion on the same occasion for metabolic studies in individuals with normal and abnormal glucose tolerance.}},
author = {{Dorkhan, Mozhgan and Tripathy, Devjit and Malm, G and Asgharian, H and Tuomi, Tiinamaija and Groop, Leif}},
issn = {{1365-2796}},
language = {{eng}},
pages = {{62--71}},
publisher = {{Wiley-Blackwell}},
series = {{Journal of Internal Medicine}},
title = {{Independent measures of insulin secretion and insulin sensitivity during the same test: the glucagon-insulin tolerance test.}},
url = {{http://dx.doi.org/10.1111/j.1365-2796.2008.01921.x}},
doi = {{10.1111/j.1365-2796.2008.01921.x}},
volume = {{264}},
year = {{2008}},
}