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Fully automated microchip system for the detection of quantal exocytosis from single and small ensembles of cells

Spegel, Christer LU ; Heiskanen, Arto LU ; Pedersen, Simon; Emnéus, Jenny LU ; Ruzgas, Tautgirdas LU and Taboryski, Rafael (2008) In Lab on A Chip 2(8). p.323-329
Abstract
A lab-on-a-chip device that enables positioning of single or small ensembles of cells on an aperture in close proximity to a mercaptopropionic acid (MPA) modified sensing electrode has been developed and characterized. The microchip was used for the detection of Ca(2+)-dependent quantal catecholamine exocytosis from single as well as small assemblies of rat pheochromocytoma (PC12) cells. The frequency of events increased considerably upon depolarization of the PC12 cell membrane using a high extracelluar concentration of potassium. The number of recorded events could be correlated with the number of cells immobilized on the electrode. Quantal characteristics, such as the number of released molecules per recorded event, are equivalent to... (More)
A lab-on-a-chip device that enables positioning of single or small ensembles of cells on an aperture in close proximity to a mercaptopropionic acid (MPA) modified sensing electrode has been developed and characterized. The microchip was used for the detection of Ca(2+)-dependent quantal catecholamine exocytosis from single as well as small assemblies of rat pheochromocytoma (PC12) cells. The frequency of events increased considerably upon depolarization of the PC12 cell membrane using a high extracelluar concentration of potassium. The number of recorded events could be correlated with the number of cells immobilized on the electrode. Quantal characteristics, such as the number of released molecules per recorded event, are equivalent to data obtained using conventional carbon fiber microelectrodes. The detection sensitivity of the device allows for the detection of less than 10 000 dopamine molecules in a quantal release. The distribution of peak rise-time and full width at half maximum was constant during measurement periods of several minutes demonstrating the stability of the MPA modified surface. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Lab on A Chip
volume
2
issue
8
pages
323 - 329
publisher
Royal Society of Chemistry
external identifiers
  • wos:000252758100017
  • scopus:38849090885
ISSN
1473-0189
DOI
10.1039/b715107a
language
English
LU publication?
yes
id
cb665ba9-3d40-4ef9-a1f7-336a2a985dac (old id 1042528)
date added to LUP
2008-03-25 16:34:01
date last changed
2017-08-20 03:41:23
@article{cb665ba9-3d40-4ef9-a1f7-336a2a985dac,
  abstract     = {A lab-on-a-chip device that enables positioning of single or small ensembles of cells on an aperture in close proximity to a mercaptopropionic acid (MPA) modified sensing electrode has been developed and characterized. The microchip was used for the detection of Ca(2+)-dependent quantal catecholamine exocytosis from single as well as small assemblies of rat pheochromocytoma (PC12) cells. The frequency of events increased considerably upon depolarization of the PC12 cell membrane using a high extracelluar concentration of potassium. The number of recorded events could be correlated with the number of cells immobilized on the electrode. Quantal characteristics, such as the number of released molecules per recorded event, are equivalent to data obtained using conventional carbon fiber microelectrodes. The detection sensitivity of the device allows for the detection of less than 10 000 dopamine molecules in a quantal release. The distribution of peak rise-time and full width at half maximum was constant during measurement periods of several minutes demonstrating the stability of the MPA modified surface.},
  author       = {Spegel, Christer and Heiskanen, Arto and Pedersen, Simon and Emnéus, Jenny and Ruzgas, Tautgirdas and Taboryski, Rafael},
  issn         = {1473-0189},
  language     = {eng},
  number       = {8},
  pages        = {323--329},
  publisher    = {Royal Society of Chemistry},
  series       = {Lab on A Chip},
  title        = {Fully automated microchip system for the detection of quantal exocytosis from single and small ensembles of cells},
  url          = {http://dx.doi.org/10.1039/b715107a},
  volume       = {2},
  year         = {2008},
}