The augmenting effect on insulin secretion by oral versus intravenous glucose is exaggerated by high-fat diet in mice.
(2008) In Journal of Endocrinology 197(1). p.181-187- Abstract
- To study whether the incretin effect is involved in adaptively increased insulin secretion in insulin resistance, glucose was infused at a variable rate to match glucose levels after oral glucose (25 mg) in normal anesthetized C57BL/6J female mice or in mice rendered insulin resistant by 8 weeks of high-fat feeding. Insulin response was markedly higher after oral than i.v. glucose in both groups, and this augmentation was even higher in high-fat fed than normal mice. In normal mice, the area under the curve (AUC(insulin)) was augmented from 4.0+/-0.8 to 8.0+/-1.8 nmol/lx60 min by the oral glucose, i.e. by a factor of 2 (P=0.023), whereas in the high-fat fed mice, AUC(insulin) was augmented from 0.70+/-0.4 to 12.4+/-2.5 nmol/lx60 min, i.e.... (More)
- To study whether the incretin effect is involved in adaptively increased insulin secretion in insulin resistance, glucose was infused at a variable rate to match glucose levels after oral glucose (25 mg) in normal anesthetized C57BL/6J female mice or in mice rendered insulin resistant by 8 weeks of high-fat feeding. Insulin response was markedly higher after oral than i.v. glucose in both groups, and this augmentation was even higher in high-fat fed than normal mice. In normal mice, the area under the curve (AUC(insulin)) was augmented from 4.0+/-0.8 to 8.0+/-1.8 nmol/lx60 min by the oral glucose, i.e. by a factor of 2 (P=0.023), whereas in the high-fat fed mice, AUC(insulin) was augmented from 0.70+/-0.4 to 12.4+/-2.5 nmol/lx60 min, i.e. by a factor of 17 (P<0.001). To examine whether the incretin hormone glucagon-like peptide-1 (GLP-1) is responsible for this difference, the effect of i.v. GLP-1 was compared in normal and high-fat fed mice. The sensitivity to i.v. GLP-1 in stimulating insulin secretion was increased in the high-fat diet fed mice: the lowest effective dose of GLP-1 was 650 pmol/kg in normal mice and 13 pmol/kg in the high-fat diet fed mice. We conclude that 1) the incretin effect contributes by approximately 50% to insulin secretion by the oral glucose in normal mice, 2) this effect is markedly exaggerated in insulin-resistant mice fed a high-fat diet, and 3) this augmented incretin contribution in the high-fat fed mice may partially be explained by GLP-1. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1052037
- author
- Ahrén, Bo LU ; Sörhede Winzell, Maria LU and Pacini, Giovanni
- organization
- publishing date
- 2008
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Journal of Endocrinology
- volume
- 197
- issue
- 1
- pages
- 181 - 187
- publisher
- Society for Endocrinology
- external identifiers
-
- pmid:18372244
- wos:000255187900018
- scopus:42449098132
- ISSN
- 1479-6805
- DOI
- 10.1677/JOE-07-0460
- language
- English
- LU publication?
- yes
- id
- 66024b41-4c7a-4f17-ba71-dd38717f526b (old id 1052037)
- alternative location
- http://www.ncbi.nlm.nih.gov/pubmed/18372244?dopt=Abstract
- date added to LUP
- 2016-04-04 07:58:39
- date last changed
- 2024-01-12 03:20:45
@article{66024b41-4c7a-4f17-ba71-dd38717f526b,
abstract = {{To study whether the incretin effect is involved in adaptively increased insulin secretion in insulin resistance, glucose was infused at a variable rate to match glucose levels after oral glucose (25 mg) in normal anesthetized C57BL/6J female mice or in mice rendered insulin resistant by 8 weeks of high-fat feeding. Insulin response was markedly higher after oral than i.v. glucose in both groups, and this augmentation was even higher in high-fat fed than normal mice. In normal mice, the area under the curve (AUC(insulin)) was augmented from 4.0+/-0.8 to 8.0+/-1.8 nmol/lx60 min by the oral glucose, i.e. by a factor of 2 (P=0.023), whereas in the high-fat fed mice, AUC(insulin) was augmented from 0.70+/-0.4 to 12.4+/-2.5 nmol/lx60 min, i.e. by a factor of 17 (P<0.001). To examine whether the incretin hormone glucagon-like peptide-1 (GLP-1) is responsible for this difference, the effect of i.v. GLP-1 was compared in normal and high-fat fed mice. The sensitivity to i.v. GLP-1 in stimulating insulin secretion was increased in the high-fat diet fed mice: the lowest effective dose of GLP-1 was 650 pmol/kg in normal mice and 13 pmol/kg in the high-fat diet fed mice. We conclude that 1) the incretin effect contributes by approximately 50% to insulin secretion by the oral glucose in normal mice, 2) this effect is markedly exaggerated in insulin-resistant mice fed a high-fat diet, and 3) this augmented incretin contribution in the high-fat fed mice may partially be explained by GLP-1.}},
author = {{Ahrén, Bo and Sörhede Winzell, Maria and Pacini, Giovanni}},
issn = {{1479-6805}},
language = {{eng}},
number = {{1}},
pages = {{181--187}},
publisher = {{Society for Endocrinology}},
series = {{Journal of Endocrinology}},
title = {{The augmenting effect on insulin secretion by oral versus intravenous glucose is exaggerated by high-fat diet in mice.}},
url = {{http://dx.doi.org/10.1677/JOE-07-0460}},
doi = {{10.1677/JOE-07-0460}},
volume = {{197}},
year = {{2008}},
}