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Polyamine synthesis inhibition induces S phase cell cycle arrest in vascular smooth muscle cells.

Odenlund, Malin LU ; Holmqvist, Bo LU ; Baldetorp, Bo LU ; Hellstrand, Per LU and Nilsson, Bengt-Olof LU (2009) In Amino Acids 36. p.273-282
Abstract
Polyamines are important for cell growth and proliferation and they are formed from arginine and ornithine via arginase and ornithine decarboxylase (ODC). Arginine may alternatively be metabolised to NO via NO synthase. Here we study if vascular smooth muscle cell proliferation can be reversed by polyamine synthesis inhibitors and investigate their mechanism of action. Cell proliferation was assessed in cultured vascular smooth muscle A7r5 cells and in endothelium-denuded rat arterial rings by measuring [(3)H]-thymidine incorporation and by cell counting. Cell cycle phase distribution was determined by flow cytometry and polyamines by HPLC. Protein expression was determined by Western blotting. The ODC inhibitor DFMO (1-10 mM) reduced... (More)
Polyamines are important for cell growth and proliferation and they are formed from arginine and ornithine via arginase and ornithine decarboxylase (ODC). Arginine may alternatively be metabolised to NO via NO synthase. Here we study if vascular smooth muscle cell proliferation can be reversed by polyamine synthesis inhibitors and investigate their mechanism of action. Cell proliferation was assessed in cultured vascular smooth muscle A7r5 cells and in endothelium-denuded rat arterial rings by measuring [(3)H]-thymidine incorporation and by cell counting. Cell cycle phase distribution was determined by flow cytometry and polyamines by HPLC. Protein expression was determined by Western blotting. The ODC inhibitor DFMO (1-10 mM) reduced polyamine concentration and attenuated proliferation in A7r5 cells and rat tail artery. DFMO accumulated cells in S phase of the cell cycle and reduced cyclin A expression. DFMO had no effect on cell viability and apoptosis as assessed by fluorescence microscopy. Polyamine concentration and cellular proliferation were not affected by the arginase inhibitor NOHA (100-200 muM) and the NO synthase inhibitor L: -NAME (100 muM). Lack of effect of NOHA was reflected by absence of arginase expression. Polyamine synthesis inhibition attenuates vascular smooth muscle cell proliferation by reducing DNA synthesis and accumulation of cells in S phase, and may be a useful approach to prevent vascular smooth muscle cell proliferation in cardiovascular diseases. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Amino Acids
volume
36
pages
273 - 282
publisher
Springer
external identifiers
  • pmid:18368465
  • wos:000263058400013
  • scopus:59449110483
ISSN
0939-4451
DOI
10.1007/s00726-008-0060-7
language
English
LU publication?
yes
id
41745c68-bda8-48d7-876d-b8614300b89c (old id 1052128)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/18368465?dopt=Abstract
date added to LUP
2008-04-02 16:29:02
date last changed
2017-10-01 04:59:07
@article{41745c68-bda8-48d7-876d-b8614300b89c,
  abstract     = {Polyamines are important for cell growth and proliferation and they are formed from arginine and ornithine via arginase and ornithine decarboxylase (ODC). Arginine may alternatively be metabolised to NO via NO synthase. Here we study if vascular smooth muscle cell proliferation can be reversed by polyamine synthesis inhibitors and investigate their mechanism of action. Cell proliferation was assessed in cultured vascular smooth muscle A7r5 cells and in endothelium-denuded rat arterial rings by measuring [(3)H]-thymidine incorporation and by cell counting. Cell cycle phase distribution was determined by flow cytometry and polyamines by HPLC. Protein expression was determined by Western blotting. The ODC inhibitor DFMO (1-10 mM) reduced polyamine concentration and attenuated proliferation in A7r5 cells and rat tail artery. DFMO accumulated cells in S phase of the cell cycle and reduced cyclin A expression. DFMO had no effect on cell viability and apoptosis as assessed by fluorescence microscopy. Polyamine concentration and cellular proliferation were not affected by the arginase inhibitor NOHA (100-200 muM) and the NO synthase inhibitor L: -NAME (100 muM). Lack of effect of NOHA was reflected by absence of arginase expression. Polyamine synthesis inhibition attenuates vascular smooth muscle cell proliferation by reducing DNA synthesis and accumulation of cells in S phase, and may be a useful approach to prevent vascular smooth muscle cell proliferation in cardiovascular diseases.},
  author       = {Odenlund, Malin and Holmqvist, Bo and Baldetorp, Bo and Hellstrand, Per and Nilsson, Bengt-Olof},
  issn         = {0939-4451},
  language     = {eng},
  pages        = {273--282},
  publisher    = {Springer},
  series       = {Amino Acids},
  title        = {Polyamine synthesis inhibition induces S phase cell cycle arrest in vascular smooth muscle cells.},
  url          = {http://dx.doi.org/10.1007/s00726-008-0060-7},
  volume       = {36},
  year         = {2009},
}