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Promoted evolution of a shortened variant of heme A synthase in the membrane of Bacillus subtilis.

Lewin, Anna LU and Hederstedt, Lars LU (2008) In FEBS Letters 582. p.1330-1334
Abstract
Bacillus subtilis heme A synthase is a membrane protein with 8 transmembrane segments. By using a two-step mutagenesis approach we have generated and selected a fully functional enzyme protein variant with a seven residue internal deletion. The biochemical properties of the shortened variant are similar to those of the normal enzyme. This could indicate that residue H209 in the mutant protein substitutes for the missing H216 as an axial ligand to the heme iron. Our results provide insight in routes of membrane protein evolution and the structure of heme A synthases.
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
CtaA, Heme A synthesis, Membrane protein evolution, Cytochrome a
in
FEBS Letters
volume
582
pages
1330 - 1334
publisher
Wiley-Blackwell
external identifiers
  • pmid:18358840
  • wos:000258004500008
  • scopus:41449097377
ISSN
1873-3468
DOI
10.1016/j.febslet.2008.03.015
language
English
LU publication?
yes
id
53b633e0-3f84-4240-b5e9-acb6645df537 (old id 1052249)
date added to LUP
2008-04-28 14:38:35
date last changed
2017-08-09 16:03:08
@article{53b633e0-3f84-4240-b5e9-acb6645df537,
  abstract     = {Bacillus subtilis heme A synthase is a membrane protein with 8 transmembrane segments. By using a two-step mutagenesis approach we have generated and selected a fully functional enzyme protein variant with a seven residue internal deletion. The biochemical properties of the shortened variant are similar to those of the normal enzyme. This could indicate that residue H209 in the mutant protein substitutes for the missing H216 as an axial ligand to the heme iron. Our results provide insight in routes of membrane protein evolution and the structure of heme A synthases.},
  author       = {Lewin, Anna and Hederstedt, Lars},
  issn         = {1873-3468},
  keyword      = {CtaA,Heme A synthesis,Membrane protein evolution,Cytochrome a},
  language     = {eng},
  pages        = {1330--1334},
  publisher    = {Wiley-Blackwell},
  series       = {FEBS Letters},
  title        = {Promoted evolution of a shortened variant of heme A synthase in the membrane of <em>Bacillus subtilis</em>.},
  url          = {http://dx.doi.org/10.1016/j.febslet.2008.03.015},
  volume       = {582},
  year         = {2008},
}