Promoted evolution of a shortened variant of heme A synthase in the membrane of Bacillus subtilis.
(2008) In FEBS Letters 582. p.1330-1334- Abstract
- Bacillus subtilis heme A synthase is a membrane protein with 8 transmembrane segments. By using a two-step mutagenesis approach we have generated and selected a fully functional enzyme protein variant with a seven residue internal deletion. The biochemical properties of the shortened variant are similar to those of the normal enzyme. This could indicate that residue H209 in the mutant protein substitutes for the missing H216 as an axial ligand to the heme iron. Our results provide insight in routes of membrane protein evolution and the structure of heme A synthases.
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1052249
- author
- Lewin, Anna LU and Hederstedt, Lars LU
- organization
- publishing date
- 2008
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- CtaA, Heme A synthesis, Membrane protein evolution, Cytochrome a
- in
- FEBS Letters
- volume
- 582
- pages
- 1330 - 1334
- publisher
- Wiley-Blackwell
- external identifiers
-
- pmid:18358840
- wos:000258004500008
- scopus:41449097377
- pmid:18358840
- ISSN
- 1873-3468
- DOI
- 10.1016/j.febslet.2008.03.015
- language
- English
- LU publication?
- yes
- id
- 53b633e0-3f84-4240-b5e9-acb6645df537 (old id 1052249)
- date added to LUP
- 2016-04-01 14:18:33
- date last changed
- 2022-01-27 23:56:29
@article{53b633e0-3f84-4240-b5e9-acb6645df537, abstract = {{Bacillus subtilis heme A synthase is a membrane protein with 8 transmembrane segments. By using a two-step mutagenesis approach we have generated and selected a fully functional enzyme protein variant with a seven residue internal deletion. The biochemical properties of the shortened variant are similar to those of the normal enzyme. This could indicate that residue H209 in the mutant protein substitutes for the missing H216 as an axial ligand to the heme iron. Our results provide insight in routes of membrane protein evolution and the structure of heme A synthases.}}, author = {{Lewin, Anna and Hederstedt, Lars}}, issn = {{1873-3468}}, keywords = {{CtaA; Heme A synthesis; Membrane protein evolution; Cytochrome a}}, language = {{eng}}, pages = {{1330--1334}}, publisher = {{Wiley-Blackwell}}, series = {{FEBS Letters}}, title = {{Promoted evolution of a shortened variant of heme A synthase in the membrane of <em>Bacillus subtilis</em>.}}, url = {{http://dx.doi.org/10.1016/j.febslet.2008.03.015}}, doi = {{10.1016/j.febslet.2008.03.015}}, volume = {{582}}, year = {{2008}}, }