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Effects of Carbon Dioxide on Neurotoxin Gene Expression in Non-proteolytic Clostridium botulinum type E.

Artin, Ingrid LU ; Carter, Andrew T; Holst, Elisabet LU ; Lövenklev, Maria LU ; Mason, David R; Peck, Michael W and Rådström, Peter LU (2008) In Applied and Environmental Microbiology 74(8). p.2391-2397
Abstract
Carbon dioxide is an antimicrobial gas commonly used in modified atmosphere packaging. In the present study, the effects of carbon dioxide on growth of, and neurotoxin production by, non-proteolytic Clostridium botulinum type E were studied during the growth cycle. Quantitative reverse transcription-PCR and an enzyme-linked immunosorbent assay were used to quantify expression of the type E botulinum neurotoxin gene (cntE) and formation of type E neurotoxin. Expression of cntE was similar in two strains, with relative expression peaking in the transition between exponential and stationary phase. In stationary phase, cntE mRNA expression declined rapidly. The cntE mRNA half-life was calculated to be approximately nine minutes. Neurotoxin... (More)
Carbon dioxide is an antimicrobial gas commonly used in modified atmosphere packaging. In the present study, the effects of carbon dioxide on growth of, and neurotoxin production by, non-proteolytic Clostridium botulinum type E were studied during the growth cycle. Quantitative reverse transcription-PCR and an enzyme-linked immunosorbent assay were used to quantify expression of the type E botulinum neurotoxin gene (cntE) and formation of type E neurotoxin. Expression of cntE was similar in two strains, with relative expression peaking in the transition between exponential and stationary phase. In stationary phase, cntE mRNA expression declined rapidly. The cntE mRNA half-life was calculated to be approximately nine minutes. Neurotoxin formation occurred in late exponential and stationary phase. High carbon dioxide concentrations delayed growth by increasing the lag time and decreasing the maximum growth rate. The effects of carbon dioxide concentration on relative neurotoxin gene expression and neurotoxin formation were significant. Expression of cntE mRNA and formation of extracellular neurotoxin were two-fold higher with a headspace carbon dioxide concentration of 70% (v/v) compared to 10% (v/v). This finding sheds a new, cautionary light on the potential risks of botulism associated with the use of modified atmosphere packaging. (Less)
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organization
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type
Contribution to journal
publication status
published
subject
in
Applied and Environmental Microbiology
volume
74
issue
8
pages
2391 - 2397
publisher
American Society for Microbiology
external identifiers
  • pmid:18310434
  • wos:000255018500016
  • scopus:42349095735
ISSN
0099-2240
DOI
10.1128/AEM.02587-07
language
English
LU publication?
yes
id
a4bfa984-fed9-491f-b96f-70ddceb453c0 (old id 1052920)
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http://www.ncbi.nlm.nih.gov/pubmed/18310434?dopt=Abstract
date added to LUP
2008-04-01 08:49:42
date last changed
2017-11-05 03:37:17
@article{a4bfa984-fed9-491f-b96f-70ddceb453c0,
  abstract     = {Carbon dioxide is an antimicrobial gas commonly used in modified atmosphere packaging. In the present study, the effects of carbon dioxide on growth of, and neurotoxin production by, non-proteolytic Clostridium botulinum type E were studied during the growth cycle. Quantitative reverse transcription-PCR and an enzyme-linked immunosorbent assay were used to quantify expression of the type E botulinum neurotoxin gene (cntE) and formation of type E neurotoxin. Expression of cntE was similar in two strains, with relative expression peaking in the transition between exponential and stationary phase. In stationary phase, cntE mRNA expression declined rapidly. The cntE mRNA half-life was calculated to be approximately nine minutes. Neurotoxin formation occurred in late exponential and stationary phase. High carbon dioxide concentrations delayed growth by increasing the lag time and decreasing the maximum growth rate. The effects of carbon dioxide concentration on relative neurotoxin gene expression and neurotoxin formation were significant. Expression of cntE mRNA and formation of extracellular neurotoxin were two-fold higher with a headspace carbon dioxide concentration of 70% (v/v) compared to 10% (v/v). This finding sheds a new, cautionary light on the potential risks of botulism associated with the use of modified atmosphere packaging.},
  author       = {Artin, Ingrid and Carter, Andrew T and Holst, Elisabet and Lövenklev, Maria and Mason, David R and Peck, Michael W and Rådström, Peter},
  issn         = {0099-2240},
  language     = {eng},
  number       = {8},
  pages        = {2391--2397},
  publisher    = {American Society for Microbiology},
  series       = {Applied and Environmental Microbiology},
  title        = {Effects of Carbon Dioxide on Neurotoxin Gene Expression in Non-proteolytic Clostridium botulinum type E.},
  url          = {http://dx.doi.org/10.1128/AEM.02587-07},
  volume       = {74},
  year         = {2008},
}