Effects of Carbon Dioxide on Neurotoxin Gene Expression in Non-proteolytic Clostridium botulinum type E.
(2008) In Applied and Environmental Microbiology 74(8). p.2391-2397- Abstract
- Carbon dioxide is an antimicrobial gas commonly used in modified atmosphere packaging. In the present study, the effects of carbon dioxide on growth of, and neurotoxin production by, non-proteolytic Clostridium botulinum type E were studied during the growth cycle. Quantitative reverse transcription-PCR and an enzyme-linked immunosorbent assay were used to quantify expression of the type E botulinum neurotoxin gene (cntE) and formation of type E neurotoxin. Expression of cntE was similar in two strains, with relative expression peaking in the transition between exponential and stationary phase. In stationary phase, cntE mRNA expression declined rapidly. The cntE mRNA half-life was calculated to be approximately nine minutes. Neurotoxin... (More)
- Carbon dioxide is an antimicrobial gas commonly used in modified atmosphere packaging. In the present study, the effects of carbon dioxide on growth of, and neurotoxin production by, non-proteolytic Clostridium botulinum type E were studied during the growth cycle. Quantitative reverse transcription-PCR and an enzyme-linked immunosorbent assay were used to quantify expression of the type E botulinum neurotoxin gene (cntE) and formation of type E neurotoxin. Expression of cntE was similar in two strains, with relative expression peaking in the transition between exponential and stationary phase. In stationary phase, cntE mRNA expression declined rapidly. The cntE mRNA half-life was calculated to be approximately nine minutes. Neurotoxin formation occurred in late exponential and stationary phase. High carbon dioxide concentrations delayed growth by increasing the lag time and decreasing the maximum growth rate. The effects of carbon dioxide concentration on relative neurotoxin gene expression and neurotoxin formation were significant. Expression of cntE mRNA and formation of extracellular neurotoxin were two-fold higher with a headspace carbon dioxide concentration of 70% (v/v) compared to 10% (v/v). This finding sheds a new, cautionary light on the potential risks of botulism associated with the use of modified atmosphere packaging. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1052920
- author
- Artin, Ingrid LU ; Carter, Andrew T ; Holst, Elisabet LU ; Lövenklev, Maria LU ; Mason, David R ; Peck, Michael W and Rådström, Peter LU
- organization
- publishing date
- 2008
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Applied and Environmental Microbiology
- volume
- 74
- issue
- 8
- pages
- 2391 - 2397
- publisher
- American Society for Microbiology
- external identifiers
-
- pmid:18310434
- wos:000255018500016
- scopus:42349095735
- ISSN
- 0099-2240
- DOI
- 10.1128/AEM.02587-07
- language
- English
- LU publication?
- yes
- id
- a4bfa984-fed9-491f-b96f-70ddceb453c0 (old id 1052920)
- alternative location
- http://www.ncbi.nlm.nih.gov/pubmed/18310434?dopt=Abstract
- date added to LUP
- 2016-04-01 12:01:37
- date last changed
- 2022-04-28 23:34:07
@article{a4bfa984-fed9-491f-b96f-70ddceb453c0, abstract = {{Carbon dioxide is an antimicrobial gas commonly used in modified atmosphere packaging. In the present study, the effects of carbon dioxide on growth of, and neurotoxin production by, non-proteolytic Clostridium botulinum type E were studied during the growth cycle. Quantitative reverse transcription-PCR and an enzyme-linked immunosorbent assay were used to quantify expression of the type E botulinum neurotoxin gene (cntE) and formation of type E neurotoxin. Expression of cntE was similar in two strains, with relative expression peaking in the transition between exponential and stationary phase. In stationary phase, cntE mRNA expression declined rapidly. The cntE mRNA half-life was calculated to be approximately nine minutes. Neurotoxin formation occurred in late exponential and stationary phase. High carbon dioxide concentrations delayed growth by increasing the lag time and decreasing the maximum growth rate. The effects of carbon dioxide concentration on relative neurotoxin gene expression and neurotoxin formation were significant. Expression of cntE mRNA and formation of extracellular neurotoxin were two-fold higher with a headspace carbon dioxide concentration of 70% (v/v) compared to 10% (v/v). This finding sheds a new, cautionary light on the potential risks of botulism associated with the use of modified atmosphere packaging.}}, author = {{Artin, Ingrid and Carter, Andrew T and Holst, Elisabet and Lövenklev, Maria and Mason, David R and Peck, Michael W and Rådström, Peter}}, issn = {{0099-2240}}, language = {{eng}}, number = {{8}}, pages = {{2391--2397}}, publisher = {{American Society for Microbiology}}, series = {{Applied and Environmental Microbiology}}, title = {{Effects of Carbon Dioxide on Neurotoxin Gene Expression in Non-proteolytic Clostridium botulinum type E.}}, url = {{http://dx.doi.org/10.1128/AEM.02587-07}}, doi = {{10.1128/AEM.02587-07}}, volume = {{74}}, year = {{2008}}, }