Integrated enzymatic reactions and analysis
(1992) In Biotechnology Techniques 6(2). p.165-168- Abstract
Enzymatic reactions were performed in a modified auto-injector unit of a Shimadzu HPLC system. The reactions were analyzed by automated injections directly into the HPLC separation system. Two reactions were studied, and the enzymes mandelonitrile lyase and α-chymotrypsin were immobilized by adsorption onto a solid support, e.g., Celite and Chromosorb. The reactions were performed in various organic solvents e.g., diisopropyl ether, heptane/ethyl acetate mixtures and acetonitrile.
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1055c566-d4f5-480f-9c77-0f98f2633e09
- author
- Wehtje, Ernst LU ; Adlercreutz, Patrick LU and Mattiasson, Bo LU
- organization
- publishing date
- 1992-03-01
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Biotechnology Techniques
- volume
- 6
- issue
- 2
- pages
- 4 pages
- publisher
- Springer
- external identifiers
-
- scopus:51649132613
- ISSN
- 0951-208X
- DOI
- 10.1007/BF02438825
- language
- English
- LU publication?
- yes
- id
- 1055c566-d4f5-480f-9c77-0f98f2633e09
- date added to LUP
- 2019-06-22 09:24:13
- date last changed
- 2021-01-03 05:20:06
@article{1055c566-d4f5-480f-9c77-0f98f2633e09, abstract = {{<p>Enzymatic reactions were performed in a modified auto-injector unit of a Shimadzu HPLC system. The reactions were analyzed by automated injections directly into the HPLC separation system. Two reactions were studied, and the enzymes mandelonitrile lyase and α-chymotrypsin were immobilized by adsorption onto a solid support, e.g., Celite and Chromosorb. The reactions were performed in various organic solvents e.g., diisopropyl ether, heptane/ethyl acetate mixtures and acetonitrile.</p>}}, author = {{Wehtje, Ernst and Adlercreutz, Patrick and Mattiasson, Bo}}, issn = {{0951-208X}}, language = {{eng}}, month = {{03}}, number = {{2}}, pages = {{165--168}}, publisher = {{Springer}}, series = {{Biotechnology Techniques}}, title = {{Integrated enzymatic reactions and analysis}}, url = {{http://dx.doi.org/10.1007/BF02438825}}, doi = {{10.1007/BF02438825}}, volume = {{6}}, year = {{1992}}, }