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Integrated enzymatic reactions and analysis

Wehtje, Ernst LU ; Adlercreutz, Patrick LU orcid and Mattiasson, Bo LU (1992) In Biotechnology Techniques 6(2). p.165-168
Abstract

Enzymatic reactions were performed in a modified auto-injector unit of a Shimadzu HPLC system. The reactions were analyzed by automated injections directly into the HPLC separation system. Two reactions were studied, and the enzymes mandelonitrile lyase and α-chymotrypsin were immobilized by adsorption onto a solid support, e.g., Celite and Chromosorb. The reactions were performed in various organic solvents e.g., diisopropyl ether, heptane/ethyl acetate mixtures and acetonitrile.

Please use this url to cite or link to this publication:
author
; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Biotechnology Techniques
volume
6
issue
2
pages
4 pages
publisher
Springer Science and Business Media B.V.
external identifiers
  • scopus:51649132613
ISSN
0951-208X
DOI
10.1007/BF02438825
language
English
LU publication?
yes
id
1055c566-d4f5-480f-9c77-0f98f2633e09
date added to LUP
2019-06-22 09:24:13
date last changed
2025-10-14 11:20:31
@article{1055c566-d4f5-480f-9c77-0f98f2633e09,
  abstract     = {{<p>Enzymatic reactions were performed in a modified auto-injector unit of a Shimadzu HPLC system. The reactions were analyzed by automated injections directly into the HPLC separation system. Two reactions were studied, and the enzymes mandelonitrile lyase and α-chymotrypsin were immobilized by adsorption onto a solid support, e.g., Celite and Chromosorb. The reactions were performed in various organic solvents e.g., diisopropyl ether, heptane/ethyl acetate mixtures and acetonitrile.</p>}},
  author       = {{Wehtje, Ernst and Adlercreutz, Patrick and Mattiasson, Bo}},
  issn         = {{0951-208X}},
  language     = {{eng}},
  month        = {{03}},
  number       = {{2}},
  pages        = {{165--168}},
  publisher    = {{Springer Science and Business Media B.V.}},
  series       = {{Biotechnology Techniques}},
  title        = {{Integrated enzymatic reactions and analysis}},
  url          = {{http://dx.doi.org/10.1007/BF02438825}},
  doi          = {{10.1007/BF02438825}},
  volume       = {{6}},
  year         = {{1992}},
}