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Association of tapasin and COPI provides a mechanism for the retrograde transport of major histocompatibility complex (MHC) class I molecules from the Golgi complex to the endoplasmic reticulum

Paulsson, Kajsa M LU ; Kleijmeer, Monique J; Griffith, Janice; Jevon, Marc; Chen, Shangwu; Anderson, Per O LU ; Sjögren, Hans Olov LU ; Li, Su-Ling LU and Wang, Ping (2002) In Journal of Biological Chemistry 277(21). p.18266-18271
Abstract

Tapasin is a subunit of the transporter associated with antigen processing (TAP). It associates with the major histocompatibility complex (MHC) class I. We show that tapasin interacts with beta- and gamma-subunits of COPI coatomer. COPI retrieves membrane proteins from the Golgi network back to the endoplasmic reticulum (ER). The COPI subunit-associated tapasin also interacts with MHC class I molecules suggesting that tapasin acts as the cargo receptor for packing MHC class I molecules as cargo proteins into COPI-coated vesicles. In tapasin mutant cells, neither TAP nor MHC class I are detected in association with the COPI coatomer. Interestingly, tapasin-associated MHC class I molecules are antigenic peptide-receptive and detected in... (More)

Tapasin is a subunit of the transporter associated with antigen processing (TAP). It associates with the major histocompatibility complex (MHC) class I. We show that tapasin interacts with beta- and gamma-subunits of COPI coatomer. COPI retrieves membrane proteins from the Golgi network back to the endoplasmic reticulum (ER). The COPI subunit-associated tapasin also interacts with MHC class I molecules suggesting that tapasin acts as the cargo receptor for packing MHC class I molecules as cargo proteins into COPI-coated vesicles. In tapasin mutant cells, neither TAP nor MHC class I are detected in association with the COPI coatomer. Interestingly, tapasin-associated MHC class I molecules are antigenic peptide-receptive and detected in both the ER and the Golgi. Our data suggest that tapasin is required for the COPI vesicle-mediated retrograde transport of immature MHC class I molecules from the Golgi network to the ER.

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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Non-U.S. Gov't, Subcellular Fractions/metabolism, Support, Histocompatibility Antigens Class I/*metabolism, Immunoglobulins/*metabolism, Golgi Apparatus/*metabolism, Endoplasmic Reticulum/*metabolism, Coat Protein Complex I/*metabolism, Cell Line, Antiporters/*metabolism, Biological Transport
in
Journal of Biological Chemistry
volume
277
issue
21
pages
6 pages
publisher
ASBMB
external identifiers
  • wos:000175975800005
  • scopus:0037166298
ISSN
1083-351X
DOI
10.1074/jbc.M201388200http://dx.doi.org/10.1074/jbc.M201388200
language
English
LU publication?
yes
id
85102695-807b-4022-b51b-54eec818806c (old id 106133)
alternative location
http://www.ncbi.nlm.nih.gov:80/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=11884415&dopt=Abstract
date added to LUP
2007-07-18 15:46:04
date last changed
2017-11-14 09:56:34
@article{85102695-807b-4022-b51b-54eec818806c,
  abstract     = {<p>Tapasin is a subunit of the transporter associated with antigen processing (TAP). It associates with the major histocompatibility complex (MHC) class I. We show that tapasin interacts with beta- and gamma-subunits of COPI coatomer. COPI retrieves membrane proteins from the Golgi network back to the endoplasmic reticulum (ER). The COPI subunit-associated tapasin also interacts with MHC class I molecules suggesting that tapasin acts as the cargo receptor for packing MHC class I molecules as cargo proteins into COPI-coated vesicles. In tapasin mutant cells, neither TAP nor MHC class I are detected in association with the COPI coatomer. Interestingly, tapasin-associated MHC class I molecules are antigenic peptide-receptive and detected in both the ER and the Golgi. Our data suggest that tapasin is required for the COPI vesicle-mediated retrograde transport of immature MHC class I molecules from the Golgi network to the ER.</p>},
  author       = {Paulsson, Kajsa M and Kleijmeer, Monique J and Griffith, Janice and Jevon, Marc and Chen, Shangwu and Anderson, Per O and Sjögren, Hans Olov and Li, Su-Ling and Wang, Ping},
  issn         = {1083-351X},
  keyword      = {Non-U.S. Gov't,Subcellular Fractions/metabolism,Support,Histocompatibility Antigens Class I/*metabolism,Immunoglobulins/*metabolism,Golgi Apparatus/*metabolism,Endoplasmic Reticulum/*metabolism,Coat Protein Complex I/*metabolism,Cell Line,Antiporters/*metabolism,Biological Transport},
  language     = {eng},
  month        = {05},
  number       = {21},
  pages        = {18266--18271},
  publisher    = {ASBMB},
  series       = {Journal of Biological Chemistry},
  title        = {Association of tapasin and COPI provides a mechanism for the retrograde transport of major histocompatibility complex (MHC) class I molecules from the Golgi complex to the endoplasmic reticulum},
  url          = {http://dx.doi.org/10.1074/jbc.M201388200http://dx.doi.org/10.1074/jbc.M201388200},
  volume       = {277},
  year         = {2002},
}