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Furfural, 5-hydroxymethyl furfural, and acetoin act as external electron acceptors during anaerobic fermentation of xylose in recombinant Saccharomyces cerevisiae.

Wahlbom, Fredrik LU and Hahn-Hägerdal, Bärbel LU (2002) In Biotechnology and Bioengineering 78(2). p.172-178
Abstract
The electron acceptors acetoin, acetaldehyde, furfural, and 5-hydroxymethylfurfural (HMF) were added to anaerobic batch fermentation of xylose by recombinant, xylose utilising Saccharomyces cerevisiae TMB 3001. The intracellular fluxes during xylose fermentation before and after acetoin addition were calculated with metabolic flux analysis. Acetoin halted xylitol excretion and decreased the flux through the oxidative pentose phosphate pathway. The yield of ethanol increased from 0.62 mol ethanol/mol xylose to 1.35 mol ethanol/mol xylose, and the cell more than doubled its specific ATP production after acetoin addition compared to fermentation of xylose only. This did, however, not result in biomass growth. The xylitol excretion was also... (More)
The electron acceptors acetoin, acetaldehyde, furfural, and 5-hydroxymethylfurfural (HMF) were added to anaerobic batch fermentation of xylose by recombinant, xylose utilising Saccharomyces cerevisiae TMB 3001. The intracellular fluxes during xylose fermentation before and after acetoin addition were calculated with metabolic flux analysis. Acetoin halted xylitol excretion and decreased the flux through the oxidative pentose phosphate pathway. The yield of ethanol increased from 0.62 mol ethanol/mol xylose to 1.35 mol ethanol/mol xylose, and the cell more than doubled its specific ATP production after acetoin addition compared to fermentation of xylose only. This did, however, not result in biomass growth. The xylitol excretion was also decreased by furfural and acetaldehyde but was unchanged by HMF. Thus, furfural present in lignocellulosic hydrolysate can be beneficial for ethanolic fermentation of xylose. Enzymatic analyses showed that the reduction of acetoin and furfural required NADH, whereas the reduction of HMF required NADPH. The enzymatic activity responsible for furfural reduction was considerably higher than for HMF reduction and also in situ furfural conversion was higher than HMF conversion. (Less)
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keywords
Comparative Study, Chromatography Liquid, Bioreactors, Biomass, Anaerobiosis : physiology, Acetoin : pharmacology, Models Chemical, NAD : metabolism, Saccharomyces cerevisiae : drug effects : genetics : metabolism, Sensitivity and Specificity, Support Non-U.S. Gov't, Xylose : metabolism, Xylitol : metabolism, Ethanol : analysis : metabolism, Fermentation : physiology, Furaldehyde : analogs & derivatives : pharmacology
in
Biotechnology and Bioengineering
volume
78
issue
2
pages
172 - 178
publisher
John Wiley & Sons
external identifiers
  • wos:000174529800007
  • pmid:11870608
  • scopus:0037140422
ISSN
1097-0290
DOI
10.1002/bit.10188
language
English
LU publication?
yes
id
15045da8-528e-4857-bf0e-8d3a29ab806a (old id 106698)
date added to LUP
2007-06-28 14:33:31
date last changed
2017-12-10 03:47:41
@article{15045da8-528e-4857-bf0e-8d3a29ab806a,
  abstract     = {The electron acceptors acetoin, acetaldehyde, furfural, and 5-hydroxymethylfurfural (HMF) were added to anaerobic batch fermentation of xylose by recombinant, xylose utilising Saccharomyces cerevisiae TMB 3001. The intracellular fluxes during xylose fermentation before and after acetoin addition were calculated with metabolic flux analysis. Acetoin halted xylitol excretion and decreased the flux through the oxidative pentose phosphate pathway. The yield of ethanol increased from 0.62 mol ethanol/mol xylose to 1.35 mol ethanol/mol xylose, and the cell more than doubled its specific ATP production after acetoin addition compared to fermentation of xylose only. This did, however, not result in biomass growth. The xylitol excretion was also decreased by furfural and acetaldehyde but was unchanged by HMF. Thus, furfural present in lignocellulosic hydrolysate can be beneficial for ethanolic fermentation of xylose. Enzymatic analyses showed that the reduction of acetoin and furfural required NADH, whereas the reduction of HMF required NADPH. The enzymatic activity responsible for furfural reduction was considerably higher than for HMF reduction and also in situ furfural conversion was higher than HMF conversion.},
  author       = {Wahlbom, Fredrik and Hahn-Hägerdal, Bärbel},
  issn         = {1097-0290},
  keyword      = {Comparative Study,Chromatography Liquid,Bioreactors,Biomass,Anaerobiosis : physiology,Acetoin : pharmacology,Models Chemical,NAD : metabolism,Saccharomyces cerevisiae : drug effects : genetics : metabolism,Sensitivity and Specificity,Support Non-U.S. Gov't,Xylose : metabolism,Xylitol : metabolism,Ethanol : analysis : metabolism,Fermentation : physiology,Furaldehyde : analogs & derivatives : pharmacology},
  language     = {eng},
  number       = {2},
  pages        = {172--178},
  publisher    = {John Wiley & Sons},
  series       = {Biotechnology and Bioengineering},
  title        = {Furfural, 5-hydroxymethyl furfural, and acetoin act as external electron acceptors during anaerobic fermentation of xylose in recombinant Saccharomyces cerevisiae.},
  url          = {http://dx.doi.org/10.1002/bit.10188},
  volume       = {78},
  year         = {2002},
}