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Genomic organization of profilin-III and evidence for a transcript expressed exclusively in testis.

Braun, Attila LU ; Aszodi, Attila LU ; Hellebrand, Heide ; Berna, Alejandro ; Fässler, Reinhard LU and Brandau, Oliver LU (2002) In Gene 283(1-2). p.219-225
Abstract
Profilins are small, widely expressed actin binding proteins, thought to be key regulators of actin dynamics in living cells. So far, three profilin-genes have been described: profilin-I (PFN1), profilin-II (PFN2) with two splice variants and the recently identified profilin-III (PFN3). Here we describe the genomic organization of the genes encoding human and mouse profilin-III. Both are single exon genes and lie in close vicinity to the renal sodium-phosphate transport gene 2 (SLC34A1, NPT2) which is highly expressed in kidney. Northern hybridization to rat tissues has previously demonstrated expression of an approximately 4.5 kb long profilin-III mRNA transcript in kidney and a mRNA transcript of approximately 1 kb in length in testis.... (More)
Profilins are small, widely expressed actin binding proteins, thought to be key regulators of actin dynamics in living cells. So far, three profilin-genes have been described: profilin-I (PFN1), profilin-II (PFN2) with two splice variants and the recently identified profilin-III (PFN3). Here we describe the genomic organization of the genes encoding human and mouse profilin-III. Both are single exon genes and lie in close vicinity to the renal sodium-phosphate transport gene 2 (SLC34A1, NPT2) which is highly expressed in kidney. Northern hybridization to rat tissues has previously demonstrated expression of an approximately 4.5 kb long profilin-III mRNA transcript in kidney and a mRNA transcript of approximately 1 kb in length in testis. Here we show that mouse profilin-III expression is restricted to testis and that the 4.2 kb profilin-III mRNA in kidney is the result of a slc34a1 transcript which includes the antisense profilin-III open reading frame in its 3prime prime or minute-untranslated region. Finally, we demonstrate by in situ hybridization that profilin-III mRNA is localized to cells in the late stage of spermatogenesis. (Less)
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keywords
In Situ Hybridization, Human, Gene Expression, Kidney : metabolism, Mice, Male, Mice Inbred Strains, Microfilament Proteins : genetics, Molecular Sequence Data, Protein Isoforms : genetics, RNA Messenger : genetics : metabolism, Rats, Reverse Transcriptase Polymerase Chain Reaction, Sequence Alignment, Sequence Homology Amino Acid, Sequence Analysis DNA, Transcription Genetic, Testis : metabolism, DNA : chemistry : genetics, DNA Complementary : chemistry : genetics, Blotting Northern, Base Sequence, Animal, Amino Acid Sequence
in
Gene
volume
283
issue
1-2
pages
219 - 225
publisher
Elsevier
external identifiers
  • wos:000174393400022
  • pmid:11867228
  • scopus:0037160530
ISSN
1879-0038
DOI
10.1016/S0378-1119(01)00855-1
language
English
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yes
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The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Pathology, (Lund) (013030000)
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d0f07697-45a5-419e-b477-a5952aab214f (old id 106719)
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http://www.ncbi.nlm.nih.gov:80/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=11867228&dopt=Abstract
date added to LUP
2016-04-01 15:31:27
date last changed
2020-12-29 04:02:47
@article{d0f07697-45a5-419e-b477-a5952aab214f,
  abstract     = {Profilins are small, widely expressed actin binding proteins, thought to be key regulators of actin dynamics in living cells. So far, three profilin-genes have been described: profilin-I (PFN1), profilin-II (PFN2) with two splice variants and the recently identified profilin-III (PFN3). Here we describe the genomic organization of the genes encoding human and mouse profilin-III. Both are single exon genes and lie in close vicinity to the renal sodium-phosphate transport gene 2 (SLC34A1, NPT2) which is highly expressed in kidney. Northern hybridization to rat tissues has previously demonstrated expression of an approximately 4.5 kb long profilin-III mRNA transcript in kidney and a mRNA transcript of approximately 1 kb in length in testis. Here we show that mouse profilin-III expression is restricted to testis and that the 4.2 kb profilin-III mRNA in kidney is the result of a slc34a1 transcript which includes the antisense profilin-III open reading frame in its 3prime prime or minute-untranslated region. Finally, we demonstrate by in situ hybridization that profilin-III mRNA is localized to cells in the late stage of spermatogenesis.},
  author       = {Braun, Attila and Aszodi, Attila and Hellebrand, Heide and Berna, Alejandro and Fässler, Reinhard and Brandau, Oliver},
  issn         = {1879-0038},
  language     = {eng},
  number       = {1-2},
  pages        = {219--225},
  publisher    = {Elsevier},
  series       = {Gene},
  title        = {Genomic organization of profilin-III and evidence for a transcript expressed exclusively in testis.},
  url          = {http://dx.doi.org/10.1016/S0378-1119(01)00855-1},
  doi          = {10.1016/S0378-1119(01)00855-1},
  volume       = {283},
  year         = {2002},
}