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Genomic organization of profilin-III and evidence for a transcript expressed exclusively in testis.

Braun, Attila LU ; Aszodi, Attila LU ; Hellebrand, Heide; Berna, Alejandro; Fässler, Reinhard LU and Brandau, Oliver LU (2002) In Gene 283(1-2). p.219-225
Abstract
Profilins are small, widely expressed actin binding proteins, thought to be key regulators of actin dynamics in living cells. So far, three profilin-genes have been described: profilin-I (PFN1), profilin-II (PFN2) with two splice variants and the recently identified profilin-III (PFN3). Here we describe the genomic organization of the genes encoding human and mouse profilin-III. Both are single exon genes and lie in close vicinity to the renal sodium-phosphate transport gene 2 (SLC34A1, NPT2) which is highly expressed in kidney. Northern hybridization to rat tissues has previously demonstrated expression of an approximately 4.5 kb long profilin-III mRNA transcript in kidney and a mRNA transcript of approximately 1 kb in length in testis.... (More)
Profilins are small, widely expressed actin binding proteins, thought to be key regulators of actin dynamics in living cells. So far, three profilin-genes have been described: profilin-I (PFN1), profilin-II (PFN2) with two splice variants and the recently identified profilin-III (PFN3). Here we describe the genomic organization of the genes encoding human and mouse profilin-III. Both are single exon genes and lie in close vicinity to the renal sodium-phosphate transport gene 2 (SLC34A1, NPT2) which is highly expressed in kidney. Northern hybridization to rat tissues has previously demonstrated expression of an approximately 4.5 kb long profilin-III mRNA transcript in kidney and a mRNA transcript of approximately 1 kb in length in testis. Here we show that mouse profilin-III expression is restricted to testis and that the 4.2 kb profilin-III mRNA in kidney is the result of a slc34a1 transcript which includes the antisense profilin-III open reading frame in its 3prime prime or minute-untranslated region. Finally, we demonstrate by in situ hybridization that profilin-III mRNA is localized to cells in the late stage of spermatogenesis. (Less)
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@article{d0f07697-45a5-419e-b477-a5952aab214f,
  abstract     = {Profilins are small, widely expressed actin binding proteins, thought to be key regulators of actin dynamics in living cells. So far, three profilin-genes have been described: profilin-I (PFN1), profilin-II (PFN2) with two splice variants and the recently identified profilin-III (PFN3). Here we describe the genomic organization of the genes encoding human and mouse profilin-III. Both are single exon genes and lie in close vicinity to the renal sodium-phosphate transport gene 2 (SLC34A1, NPT2) which is highly expressed in kidney. Northern hybridization to rat tissues has previously demonstrated expression of an approximately 4.5 kb long profilin-III mRNA transcript in kidney and a mRNA transcript of approximately 1 kb in length in testis. Here we show that mouse profilin-III expression is restricted to testis and that the 4.2 kb profilin-III mRNA in kidney is the result of a slc34a1 transcript which includes the antisense profilin-III open reading frame in its 3prime prime or minute-untranslated region. Finally, we demonstrate by in situ hybridization that profilin-III mRNA is localized to cells in the late stage of spermatogenesis.},
  author       = {Braun, Attila and Aszodi, Attila and Hellebrand, Heide and Berna, Alejandro and Fässler, Reinhard and Brandau, Oliver},
  issn         = {1879-0038},
  keyword      = {In Situ Hybridization,Human,Gene Expression,Kidney : metabolism,Mice,Male,Mice Inbred Strains,Microfilament Proteins : genetics,Molecular Sequence Data,Protein Isoforms : genetics,RNA Messenger : genetics : metabolism,Rats,Reverse Transcriptase Polymerase Chain Reaction,Sequence Alignment,Sequence Homology Amino Acid,Sequence Analysis DNA,Transcription Genetic,Testis : metabolism,DNA : chemistry : genetics,DNA Complementary : chemistry : genetics,Blotting Northern,Base Sequence,Animal,Amino Acid Sequence},
  language     = {eng},
  number       = {1-2},
  pages        = {219--225},
  publisher    = {Elsevier},
  series       = {Gene},
  title        = {Genomic organization of profilin-III and evidence for a transcript expressed exclusively in testis.},
  url          = {http://dx.doi.org/10.1016/S0378-1119(01)00855-1},
  volume       = {283},
  year         = {2002},
}